Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
This product is useful for ELISA and SDS-Page. The purity of this protein is > 95% by SDS - PAGE. This recombinant protein was expressed in E.Coli bacteria. The endotoxin levels < 1.0 EU per 1 microgram of protein (determined by LAL method).
Dilutions:
ELISA, SDS-Page
TMW:
31.4 kDa
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Notes:
Endotoxin Level: < 1.0 EU per 1 ug of protein (determined by LAL method)
Packaging:
Storage:
Store at -80 °C. Avoid freeze-thaw cycles.
Buffer:
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 50mM NaCl, 0.1mM PMSF, 10% glycerol
Preservative:
No Preservative
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Heme oxygenase 1 belongs to the heme oxygenase family and is an essential enzyme in heme catabolism. It cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Also this protein is known to play an important role in the regulation of cardiovascular function and its adaptive response to a variety of stressors. Recombinant human Heme oxygenase 1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.