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This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 32kDa.
Theoretical MW
32 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternate Names for HO-1/HMOX1/HSP32 Antibody (EP1391Y)
bK286B10
EC 1.14.99.3
heat shock protein, 32-kD
heme oxygenase (decycling) 1
HMOX1
HO
HO1
HO-1
HO-1heme oxygenase 1
HSP32
Background
Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Product General Protocols
View specific protocols for HO-1/HMOX1/HSP32 Antibody (NB110-57028):
FAQs for HO-1/HMOX1/HSP32 Antibody (NB110-57028). (Showing 1 - 1 of 1 FAQs).
Our customer has worked in the clinical laboratory in hospital. She want to use NB110-57028 (Heme Oxygenase 1) and NBP1-19775 (IL-1 beta) for the first time. She want to know which tissue (ex, liver...) is usually used as a positive control for IHC-(p) for the good result. Because she has several tissues from human sample to test. And then, could you recommend items for IL-17 and TNF alpha antibody for IHC (p), human sample for her?
For NBP1-19775, the best positive control would be human lung carcinoma. IL1 beta is produced by activated macrophages, so any sample invaded by stimulated macrophages would be acceptable. A good positive control for NB110-57028 are spleen samples or live samples from animals that were treated with heavy metals or other compounds like endotoxin. You may use the following links to chose IL 7 and TNF alpha antibodies which will match customer's mentioned criteria: IL 7 Antibodies, TNF Antibodies.
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