Hela Whole Cell Lysate (NB800-PC1)
Hela Whole Cell Lysate
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Distributor Data...
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Hela Whole Cell Lysate Summary: | | | Specificity: | NB800_PC1 contains HeLa whole cell extract lysate. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Hela Whole Cell Products Lysate Details: | Type: | Cell | Life Stage: | Adult | | Tissue: | Reproductive | Tissue Condition: | Normal | | Sub-cellular Fraction: | Whole |
| Uses: | Lysates are prepared as positive controls for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95 degrees C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. |
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| Dilutions: | Western Blot |
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| Unit Size: | 1.0 mg |
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| Concentration: | 2.5 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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Notes: Protein supplied in 1x Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 5% prior to use. Packaging: | Storage: | Store at -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 1X SDS-PAGE Sample Buffer [62.5 mM Tris HCl, 2% SDS, 10% Glycerol, 0.005% bromophenol blue, pH 6.8] |
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| Preservative: | No Preservative |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: NB800-PC1 whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
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Images (2) Reviews (0) Related Products (4)
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Working with Hela Whole Cell
Hela Whole Cell Lysate Images (2) Western Blot: Hela Whole Cell Lysate [NB800-PC1] - Western blot analysis of STIM-1 antibody (NB110-60547) using HeLa whole cell lysate [NB800-PC1]. |  Ponceau S: Hela Whole Cell Lysate [NB800-PC1] - Ponceau S staining of HeLa whole cell lysate using NB800-PC1. |
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Jump to: Positive Controls, Lysates
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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Other Positive Controls- HeLa is only applicable as a positive control for specific antibodies.
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Lysates (4)
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