HSD3B1 Antibody (FDO66Q) 0.1 ml Trophoblast-associated Marker

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Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.
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Ordering Information: NB110-78644

  • Catalog Number
    NB110-78644
  • Displayed Format
    Unconjugated
  • Other Formats
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HSD3B1 Antibody (FDO66Q) Summary

Species Human, Rat, Baboon, Bovine, Primate
Tested Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Monoclonal
Host
Mouse
Gene
HSD3B1
Purity
Protein G purified
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HSD3B1 Antibody (FDO66Q) Details

Clone
FDO66Q
Isotype
IgG1
Marker
Trophoblast-associated Marker
Immunogen
JEG Choriocarcinoma cells
Localization
Membrane

Species Reactivity

Human, baboon, bovine, rat and marmoset.
Publications Read Publications using NB110-78644 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunohistochemistry 10-20 ug/ml
  • Immunohistochemistry-Frozen 10-20 ug/ml
  • Immunohistochemistry-Paraffin 10-20 ug/ml
Application Notes
This HSD3B1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections and frozen sections. There has been negative feedback with the use of this antibody in Western blot.
Positive Controls
Other Available
Formats
7C Labeled NB110-78644V2
Alexa Fluor (R) 405 Labeled NB110-78644AF405
Alexa Fluor (R) 488 Labeled NB110-78644AF488
Alexa Fluor (R) 647 Labeled NB110-78644AF647
Alexa Fluor (R) 700 Labeled NB110-78644AF700
Allophycocyanin Labeled NB110-78644APC
Biotin Labeled NB110-78644B
DyLight 350 Labeled NB110-78644UV
DyLight 405 Labeled NB110-78644V
DyLight 405LS Labeled NB110-78644V3
DyLight 488 Labeled NB110-78644G
DyLight 550 Labeled NB110-78644R
DyLight 650 Labeled NB110-78644C
DyLight 680 Labeled NB110-78644FR
DyLight 755 Labeled NB110-78644IR
FITC Labeled NB110-78644F
HRP Labeled NB110-78644H
PE Labeled NB110-78644PE
PerCP Labeled NB110-78644PCP
Publications
Read Publications using
NB110-78644 in the following applications:

Contact Information

Product PDFs

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Publications

HSD3B1 Antibody (NB110-78644) has been mentioned in at least 4 publications.
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-glycine, 150 mM NaCl
Unit Size
0.1 ml
Concentration
1 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol HSD3B1
Entrez

Alternate Names for HSD3B1 Antibody (FDO66Q)

  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
  • 3BETAHSD
  • 3-beta-HSD I
  • 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
  • 3BH
  • delta-5-3-ketosteroid isomerase
  • EC 1.1.1
  • HSD3B
  • HSDB3
  • HSDB3A
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1
  • I
  • progesterone reductase
  • SDR11E1
  • short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase
  • steroid Delta-isomerase
  • Trophoblast antigen FDO161G
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Related Products by Gene

Background

3A-hydroxysteroid dehydrogenase (HSD3B) is a bifunctional enzyme from 3-beta-HSD family that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid as well as ketosteroids, and is essential for the biosynthesis of all classes of hormonal steroids. HSD3B catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosteron. HSD3B is highly expressed in placenta, skin, mammary tissues etc and localize to ER/mitochondrion membranes as single-pass membrane protein wherein it plays critical role in lipid metabolism as well as steroid biosynthesis pathways. Among its different isoforms, HSD3B1 is abundantly expressed in human placenta and is implicated progesterone biosynthesis for the maintenance of pregnancy. HSD3B1 polymorphism has been linked to prostate cancer susceptibility, an increased risk of benign prostate hyperplasia, and essential hypertension.

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Publications for HSD3B1 Antibody (NB110-78644) (4)

Read what people are writing who have used HSD3B1 Antibody (NB110-78644).

We have publications tested in 2 confirmed species: Rat, Bovine.

We have publications tested in 3 applications: WB, ICC/IF, IHC-Fr.

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Product General Protocols

View specific protocols for HSD3B1 Antibody (NB110-78644): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for HSD3B1 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)