HSD3B1 Antibody (FDO66Q) Summary
||Human, Rat, Baboon, Bovine, Primate
||WB, ICC/IF, IHC, IHC-Fr, IHC-P
Protein G purified
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HSD3B1 Antibody (FDO66Q) Details
JEG Choriocarcinoma cells
Human, baboon, bovine, rat and marmoset.
||Read Publications using NB110-78644 in the following species:
- Western Blot
- Immunocytochemistry/Immunofluorescence 10-20 ug/ml
- Immunohistochemistry 10-20 ug/ml
- Immunohistochemistry-Frozen 10-20 ug/ml
- Immunohistochemistry-Paraffin 10-20 ug/ml
This HSD3B1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections and frozen sections. There has been negative feedback with the use of this antibody in Western blot.
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HSD3B1 Antibody (NB110-78644) has been mentioned in at least 4 publications
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Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Alternate Names for HSD3B1 Antibody (FDO66Q)
- 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
- 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
- 3-beta-HSD I
- 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
Related Products by Gene
3A-hydroxysteroid dehydrogenase (HSD3B) is a bifunctional enzyme from 3-beta-HSD family that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid as well as ketosteroids, and is essential for the biosynthesis of all classes of hormonal steroids. HSD3B catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosteron. HSD3B is highly expressed in placenta, skin, mammary tissues etc and localize to ER/mitochondrion membranes as single-pass membrane protein wherein it plays critical role in lipid metabolism as well as steroid biosynthesis pathways. Among its different isoforms, HSD3B1 is abundantly expressed in human placenta and is implicated progesterone biosynthesis for the maintenance of pregnancy. HSD3B1 polymorphism has been linked to prostate cancer susceptibility, an increased risk of benign prostate hyperplasia, and essential hypertension.
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Publications for HSD3B1 Antibody (NB110-78644) (4)
Read what people are writing who have used HSD3B1 Antibody (NB110-78644).
We have publications tested in 2 confirmed species: Rat, Bovine.
We have publications tested in 3 applications: WB, ICC/IF, IHC-Fr.
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|Mueller, UW et al. Isolation of fetal trophoblast cells from peripheral blood of pregnant women. Lancet;336(8709):197-200. 1990 Jul 28. [PMID: 1973769] (ICC/IF)
|Irving-Rodgers HF, Bathgate RA, Ivell R, Domagalski R, Rodgers RJ. Dynamic changes in the expression of relaxin-like factor (INSL3), cholesterol side-chain cleavage cytochrome p450, and 3beta-hydroxysteroid dehydrogenase in bovine ovarian follicles during growth and atresia. Biol Reprod;66(4):934-43. 2002 Apr. [PMID: 11906911] (IHC-Fr, Bovine)
|Irving-Rodgers HF, Krupa M, Rodgers RJ. Cholesterol side-chain cleavage cytochrome P450 and 3beta-hydroxysteroid dehydrogenase expression and the concentrations of steroid hormones in the follicular fluids of different phenotypes of healthy and atretic bovine ovarian follicles. Biol Reprod;69(6):2022-8. 2003 Dec. [PMID: 12930727]
|Palaniappan M, Menon KM. Luteinizing Hormone/Human Chorionic Gonadotropin-Mediated Activation of mTORC1 Signaling Is Required for Androgen Synthesis by Theca-Interstitial Cells Mol Endocrinol 2012 Oct [PMID: 22827930] (WB, Rat)
Product General Protocols
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FAQs for HSD3B1 (2)
- In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
- For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
- I was wondering what does Format 7C mean as far as anitbodies go?
- 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Bioinformatics Tool for HSD3B1
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