HP1 alpha Antibody (MAC385) 2 ml Heterochromatin Marker

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Ordering Information: SM1543

  • Catalog Number
    SM1543
  • Displayed Format
    Unconjugated
  • Size(s)
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    HP1 alpha Antibody (MAC385) Summary

    Species Human, Mouse
    Tested Applications WB, ELISA, IHC, IHC-Fr
    Clonality
    Monoclonal
    Host
    Rat
    Gene
    CBX5
    Purity
    Tissue culture supernatant
    Specificity
    Please Note: Catalog Number SM1543 has been replaced with NBP2-34835. Please contact Novus Biologicals with questions.
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    HP1 alpha Antibody (MAC385) Details

    Clone
    MAC385
    Isotype
    IgM
    Marker
    Heterochromatin Marker
    Immunogen
    Full length M32 fusion protein
    Localization
    Nuclear

    Species Reactivity

    Human and mouse. Other species not tested.

    Applications/Dilutions

    Dilutions
    • Western Blot 1:100-1:2000
    • ELISA 1:100-1:2000
    • Immunohistochemistry 1:10-1:500
    • Immunohistochemistry-Frozen 1:10-1:500
    Application Notes
    Immunohistochemistry of frozen sections. ELISA. Western blotting. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.

    Contact Information

    Product PDFs

    Reviews

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    Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Unit Size
    2 ml
    Concentration
    This product is unpurified. The exact concentration of antibody is not quantifiable.
    Preservative
    0.09% Sodium Azide
    Limitations
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

    Bioinformatics

    Gene Symbol CBX5
    Entrez

    Alternate Names for HP1 alpha Antibody (MAC385)

    • Antigen p25
    • chromobox homolog 5
    • chromobox homolog 5 (Drosophila HP1 alpha)
    • chromobox homolog 5 (HP1 alpha homolog, Drosophila)
    • chromobox protein homolog 5
    • Heterochromatin protein 1 homolog alpha
    • heterochromatin protein 1-alpha
    • HP1
    • HP1 alpha
    • HP1 alpha homolog
    • HP1A
    • HP1-ALPHA
    • HP1Hs alpha
    • HP1Hs-alpha
    Show More

    Related Products by Gene

    Background

    Heterochromatin protein 1 (HP1) is localized at heterochromatin sites, where it mediates gene silencing. A component of heterochromatin, HP1 recognizes and binds histone H3, leading to epigenetic repression. HP1 may interact with lamin B receptor (LBR), and this interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. The Drosophila HP1 protein, product of the Su(var)2-5 locus, is a dose dependent regulator of gene silencing. HP1 interacts with compacted chromatin (heterochromatin) via its chromodomain (CD). The HP1 protein is also known to interact with a number of transcriptional regulators via its chromoshadow (CSD) domain, suggesting that HP1 is a bridging factor that couples particular chromatin structures with effects on gene expression.

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    Publications for HP1 alpha Antibody (SM1543) (0)

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol

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    FAQs for HP1 alpha (2)

    1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
      • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
    2. I was wondering what does Format 7C mean as far as anitbodies go?
      • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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