HNF4 alpha Antibody (H00003172-M04)

HNF4 alpha Antibody (4E2)

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HNF4 alpha Antibody (4E2) Summary:
Species:Hu
Applications:ELISA, IF, RNAi, WB
Clonality:Monoclonal
Gene:HNF4A
Purity:IgG purified
Host:Mouse
Specificity:HNF4A (4E2)
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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HNF4 alpha Antibody (4E2) Details:
Clone:4E2
Isotype:IgG2a Kappa
Immunogen:HNF4A (NP_000448, 324 a.a. ~ 424 a.a) partial recombinant protein with GST tag.
Epitope:NDRQYDSRGRFGELLLLLPTLQSITWQMIE QIQFIKLFGMAKIDNLLQEMLLGGSPSDAP HAHHPLHPHLMQEHMGTNVIVANTMPTHLS NGQMCEWPRP*
Species Reactivity:

Human. Other species not tested.

Applications:
Uses:Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA.
Dilutions:ELISA, immunofluorescence, Western Blot 1:500, RNAi Validation
Unit Size:0.1 mg
Concentration:Please see the vial label for concentration.
Notes:
This product is produced by and distributed for Abnova, a company based in Taiwan.
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:In 1x PBS, pH 7.2
Preservative:No Preservative
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: HNF4A
Entrez3172 (Human)
OMIM125850, 125853,
Swiss ProtNP_000448 (Human)
 
Background:

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

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Working with HNF4 alpha

HNF4 alpha Antibody (4E2) Images (6)
Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi or non-transfected control. Blot probed with H00003172-M04. GAPDH (36.1 kDa) used as loading control.Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody (M04), clone 4E2.

Lane 1: HNF4A transfected lysate(51.6 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence of monoclonal antibody to HNF4A (H00003172-M04) on HeLa cell . [antibody concentration 10 ug/ml]HNF4A monoclonal antibody (M04), clone 4E2. Western Blot analysis of HNF4A expression in Jurkat ( Cat # L017V1 ).
HNF4A monoclonal antibody (M04), clone 4E2 Western Blot analysis of HNF4A expression in HepG2 ( Cat # L019V1 ).Detection limit for recombinant GST tagged HNF4A is approximately 1ng/ml as a capture antibody.
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Jump to: Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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