HNF-4 alpha/NR2A1 Antibody 0.1 ml

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Western Blot: HNF4 alpha Antibody [NBP1-89679] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Negative control (vector only transfected HEK293T lysate)<br/>Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400161)
Immunohistochemistry-Paraffin: HNF4 alpha Antibody [NBP1-89679] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
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HNF-4 alpha/NR2A1 Antibody Summary

Species Human
Predicted Species
Mouse (91%), Rat (92%). Backed by our 100% Guarantee
Tested Applications WB, IHC, IHC-P
Immunogen affinity purified
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
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HNF-4 alpha/NR2A1 Antibody Details



  • Western Blot 1:100-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin HIER pH6 retrieval is recommended.
Positive Controls
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NBP1-89679 in the following applications:

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    Packaging, Storage & Formulations

    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    PBS, pH 7.2, containing 40% glycerol
    Unit Size
    0.1 ml
    Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
    0.02% Sodium Azide
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.


    Gene Symbol HNF4A

    Alternate Names for HNF-4 alpha/NR2A1 Antibody

    • FLJ39654
    • hepatic nuclear factor 4 alpha
    • hepatocyte nuclear factor 4, alpha
    • hepatocyte nuclear factor 4-alpha
    • HNF4a7
    • HNF-4-alpha
    • HNF4alpha10/11/12
    • HNF4HNF4a8
    • MODY
    • MODY1
    • NR2A1HNF4a9
    • NR2A21
    • Nuclear receptor subfamily 2 group A member 1
    • TCF
    • TCF-14
    • TCF14HNF4alpha
    • Transcription factor 14
    • Transcription factor HNF-4
    • transcription factor-14
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    Publications for HNF-4 alpha/NR2A1 Antibody (NBP1-89679) (1)

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    Product General Protocols

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    WB Video Protocol

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    FAQs for HNF-4 alpha/NR2A1 (2)

    1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
      • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
    2. I was wondering what does Format 7C mean as far as anitbodies go?
      • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)