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Ordering Information: NBP1-89679
HNF-4 alpha/NR2A1 Antibody Summary
Mouse (91%), Rat (92%). Backed by our 100% Guarantee
WB, IHC, IHC-P
Immunogen affinity purified
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Test in a species/application not listed above to receive a full credit towards a future purchase.
HNF-4 alpha/NR2A1 Antibody Details
This antibody was developed against Recombinant Protein corresponding to amino acids:LLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEV
Western Blot 1:100-1:500 Immunohistochemistry 1:10-1:500 Immunohistochemistry-Paraffin 1:50-1:200
For IHC-Paraffin HIER pH6 retrieval is recommended.
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Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, pH 7.2, containing 40% glycerol
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
0.02% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
for 1 year from date of receipt.
Alternate Names for HNF-4 alpha/NR2A1 Antibody
HNF4A MODY1 NR2A1 TCF14 FLJ39654 Show More Related Products by Gene
HEPATOCYTE NUCLEAR FACTOR 1
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Product General Protocols
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FAQs for HNF-4 alpha/NR2A1 (2)
In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit:
IHC-P Protocol and IHC-P Troubleshooting. I was wondering what does Format 7C mean as far as anitbodies go?
7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
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