HNF-4 alpha/NR2A1 Antibody 0.1 ml

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Western Blot: HNF4 alpha Antibody [NBP1-89679] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Negative control (vector only transfected HEK293T lysate)<br/>Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400161)
Immunohistochemistry-Paraffin: HNF4 alpha Antibody [NBP1-89679] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
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Ordering Information: NBP1-89679

  • Catalog Number
    NBP1-89679
  • Displayed Format
    Unconjugated
  • Size(s)
    0.1 ml
  • Availability
  • Price
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HNF-4 alpha/NR2A1 Antibody Summary

Species Human
Predicted Species
Mouse (91%), Rat (92%). Backed by our 100% Guarantee
Tested Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
HNF4A
Purity
Immunogen affinity purified
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
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HNF-4 alpha/NR2A1 Antibody Details

Isotype
IgG
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:LLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEV

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin HIER pH6 retrieval is recommended.
Positive Controls
Publications
Read a Publication using
NBP1-89679 in the following applications:

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    Publications

    HNF-4 alpha/NR2A1 Antibody (NBP1-89679) has been mentioned in at least 1 publication.
    Earn rewards if you have published using HNF-4 alpha/NR2A1 Antibody (NBP1-89679).

    Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS, pH 7.2, containing 40% glycerol
    Unit Size
    0.1 ml
    Concentration
    Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
    Preservative
    0.02% Sodium Azide
    Limitations
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

    Bioinformatics

    Gene Symbol HNF4A
    Entrez
    Uniprot

    Alternate Names for HNF-4 alpha/NR2A1 Antibody

    • HNF4A
    • MODY1
    • NR2A1
    • TCF14
    • FLJ39654
    • hepatic nuclear factor 4 alpha
    • hepatocyte nuclear factor 4, alpha
    • hepatocyte nuclear factor 4-alpha
    • HNF4a7
    • HNF-4-alpha
    • HNF4alpha10/11/12
    • HNF4HNF4a8
    • MODY
    • MODY1
    • NR2A1HNF4a9
    • NR2A21
    • Nuclear receptor subfamily 2 group A member 1
    • TCF
    • TCF-14
    • TCF14HNF4alpha
    • Transcription factor 14
    • Transcription factor HNF-4
    • transcription factor-14
    Show More

    Related Products by Gene


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    Publications for HNF-4 alpha/NR2A1 Antibody (NBP1-89679) (1)

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol

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    FAQs for HNF-4 alpha/NR2A1 (2)

    1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
      • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
    2. I was wondering what does Format 7C mean as far as anitbodies go?
      • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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