HIF-2 alpha Antibody (NB100-122)
HIF-2 alpha Antibody
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Distributor Data...
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anti-Class E basic helix-loop-helix protein 73 antibody, anti-MOP 2 antibody, anti-Member of PAS protein 2 antibody, anti-Hypoxia inducible factor 2 alpha antibody, anti-HIF1 alpha like factor antibody, anti-HIF 1 alpha like factor antibody, anti-Endothelial PAS domain containing protein 1 antibody, anti-ECYT4 antibody, anti-bHLHe73 antibody, anti-Basic helix loop helix PAS protein MOP2 antibody, anti-EPAS-1 antibody, anti-Endothelial pas domain protein 1 antibody, anti-PASD2 antibody, anti-MOP2 antibody, anti-Member of pas superfamily 2 antibody, anti-Hypoxia inducible factor 2 alpha subunit antibody, anti-HLF antibody, anti-Hif-2a antibody, anti-Hif2a antibody, anti-Hif 2a antibody, anti-Hif 2 alpha antibody, anti-EPAS1 antibody |
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HIF-2 alpha Antibody Summary: | | Gene: | EPAS1 |
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| Purity: | Peptide affinity purified |
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| Host: | Rabbit |
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| | Specificity: | Specific for HIF-2 alpha/EPAS. Does not cross-react with HIF-1 alpha. |
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| Publications: | Antibody has been mentioned in at least 147 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional HIF-2 alpha Products HIF-2 alpha Antibody Details: | Immunogen: | A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein. |
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Mouse, rat, fish and human HIF-2 alpha. |
| Uses: | Used in Western blot, this antibody recognizes a band at 118kDa representing HIF-2 alpha. Also useful for immunoprecipitation and Chromatin immunoprecipitation. |
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| Dilutions: | Chromatin Immunoprecipitation, ELISA, Immunocytochemistry 1:100, Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100, Immunoprecipitation 5ug/1mg lysate, Western Blot 1:200-1:1000 |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 1.0 mg/ml |
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| Positive Controls: | 3 Positive Controls |
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| Conjugated Antibodies: | 5 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Tris-glycine, 150mM NaCl pH 7.5 |
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| Preservative: | 0.05% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.
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Images (4) Reviews (5) Related Products (22) Exploring HIF-2 alpha Working with HIF-2 alpha
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with HIF-2 alpha
HIF-2 alpha Antibody Images (4) Western Blot: HIF-2 alpha Antibody [NB100-122] - Western blot analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using NB100-122. |  Immunocytochemistry: HIF-2 alpha Antibody [NB100-122] - Detection of HIF-2 Alpha (Green) in RCC4 cells using NB100-122. Nuclei (Blue) are counterstained with Hoechst 33258. |  Western Blot: HIF-2 alpha Antibody [NB100-122] - Western Blot analysis on normoxic and hypoxic nuclear rat cell lysates using NB100-122, anti-HIF-2 alpha. |  Immunohistochemistry: HIF-2 alpha antibody [NB100-122] - Hif-2 alpha immunoreactivity in human cardiac myocytes stained with NB100-122. |
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Reviewed by:
Li-Wen Chen 12/8/2011
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Application:
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Western Blot
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Sample Tested:
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Mouse
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Species:
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Mouse
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Blocking Buffer:
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10% Milk
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Dilution Ratio:
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1:1000
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Incubation Time:
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O/N
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Incubation Temperature:
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4 Celsius
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Reviewed by:
Anonymous 6/15/2011
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Application:
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Western Blot
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Sample Tested:
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ACHN, SN12C, Caki1 whole cell lysates
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Sample Loaded:
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30ug
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Species:
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Human
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Sample Pretreated:
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Hypoxia
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Lot:
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BC3
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Results:
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Antibody at 1:500 dilution detects HIF2 alpha at the appropriate molecular weight (around 94kDa) in cells exposed to hypoxia (0.5% O2, 16h). No need to do nuclear extracts, it works for total cell protein extracts, also the amount of protein for lane should be 30-80ug. Usually 30 ug is enough.
Good luck!!!
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Pub Med ID:
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21233420
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Blocking Buffer:
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5% Milk in TBST
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Concentration:
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5%
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Blocking Time:
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1h
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Blocking Temperature:
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RT, 22 degrees Celsius
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Dilution Ratio:
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1:500
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Incubation Time:
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o/n
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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same as blocking
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Description:
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anti-Rabbit-HRP from Pierce
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Manufacturer Catalog #:
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31460
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Dilution Ratio:
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1:5000
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Method:
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ECL
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Exposure Time:
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3 min
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Positive Control:
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Hypoxia treatment
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Negative Control:
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Normoxia (21% O2) treatment
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Did you observe bands?:
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Yes
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Specific Bands:
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94 kDa
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Non-specific Bands:
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none kDa
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Reviewed by:
Anonymous 2/2/2009
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Application:
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IHC-P
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Sample Tested:
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human melanoma
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Species:
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Human
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Lot:
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AI
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Results:
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Staining is positive but relatively weak. Positive staining was cytoplasmic in some areas and nuclear in others. The Novus antibody NB100-480 that is supposed to work only for WB worked better than 100-122 when used with the same protocol on a serial section.
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Blocking Buffer:
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heat inactivated goat serum in PBS
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Blocking Time:
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30 min
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Blocking Temperature:
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room temperature
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Dilution Ratio:
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1/100
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Incubation Time:
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overnight
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Incubation Temperature:
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4 4
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Incubation Dilution Buffer:
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blocking buffer
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Description:
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anti rabbit santa cruz
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Manufacturer Catalog #:
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1/200
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Reviewed by:
Anonymous 2/9/2009
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Application:
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Western Blot
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Sample Tested:
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head and neck squamous cell carcinoma cells
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Sample Loaded:
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35 ug
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Blocking Buffer:
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nonfat dry milk in TBS-T
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Concentration:
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5%
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Blocking Time:
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1 hr
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Blocking Temperature:
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25 Celsius
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Dilution Ratio:
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1/500
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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5% nonfat dry milk in TBS-T
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Method:
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ECL
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Exposure Time:
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varies
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Positive Control:
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hypoxia-treated sample
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Negative Control:
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normoxia
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Did you observe bands?:
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True
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Specific Bands:
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~120 kDa
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Non-specific Bands:
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none kDa
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Reviewed by:
Anonymous 3/11/2010
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Application:
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Western Blot
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Sample Tested:
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Colon Cancer Cell llines
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Sample Loaded:
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50ug
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Species:
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Other
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Species Other:
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Other
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Lot:
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AT1
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Results:
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The image was of RKO cells. Using HCT116 cells we saw multiple bands, but the band at 118 was deinitely stronger. We image out westerns using a Tyhpoon and ECL Plus. We find we can use more dilute antibodies than when we were using regular ECL and film.
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Blocking Buffer:
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Blotto
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Concentration:
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5%
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Blocking Time:
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1hr
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Blocking Temperature:
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RT
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Dilution Ratio:
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1/2000
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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5% BSA in TNS with Tween
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Description:
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anti-Rabbit
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Dilution Ratio:
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1/10000
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Method:
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ECL Plus
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Specific Bands:
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118 kDa
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Publications (147)HIF-2 alpha Antibody Product Specific:- Peng YJ, Nanduri J, Khan SA, et al. Hypoxia-inducible factor 2{alpha} (HIF-2{alpha}) heterozygous-null mice exhibit exaggerated carotid body sensitivity to hypoxia, breathing instability, and hypertension. Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):3065-70.[PMID 21288809]
more... - Place TL, Fitzgerald MP, Venkataraman S, et al. Aberrant Promoter CpG Methylation Is a Mechanism for Impaired PHD3 Expression in a Diverse Set of Malignant Cells. PLoS One. 6(1).[PMID 21297970]
- Bohonowych JE, Peng S, Gopal U, et al. Comparative analysis of novel and conventional Hsp90 inhibitors on HIF activity and angiogenic potential in clear cell renal cell carcinoma: implications for clinical evaluation. BMC Cancer. 2011 Dec 15;11(1):520. [PMID: 22172030]
- Metallo CM, Gameiro PA, Bell EL, et al. Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia. Nature. 2011 Nov 20. doi: 10.1038/nature10602. [PMID 22101433]
- Wang H, Gillis A, Zhao C, et al. Crocidolite asbestos-induced signal pathway dysregulation in mesothelial cells. Mutat Res. 2011 Aug 16;723(2):171-6.[PMID 21570478]
- Shen GM, Zhao YZ, Chen MT, et al. Hypoxia inducible factor 1 (HIF1) promotes LDL and VLDL uptake through inducing VLDLR under hypoxia.Biochem J. 2011 Oct 4. [PMID: 21970364]
- Xie L, Collins JF. Transcriptional Regulation of the Menkes Copper ATPase (Atp7a) Gene by Hypoxia Inducible Factor (Hif2α) in Intestinal Epithelial Cells. Am J Physiol Cell Physiol. 2011 Feb 23. [PMID: 21346155]
- Lange C, Heynen SR, Tanimoto N, et al. Normoxic activation of hypoxia inducible factors in photoreceptors provides transient protection against light induced retinal degeneration. Invest Ophthalmol Vis Sci. 2011 Mar 29.[PMID 21447692]
- Nagao K, Oka K. HIF-2 directly activates CD82 gene expression in endothelial cells. Biochem Biophys Res Commun. 2011 Mar 4.[PMID 21382346]
- Copple BL, Bai S, Burgoon LD, Moon J-O. Hypoxia-inducible factor-1[alpha] regulates the expression of genes in hypoxic hepatic stellate cells important for collagen deposition and angiogenesis. Liver Int. 2011 Feb.[PMID 20880076]
- Higashimura Y, Terai T, Yamaji R, et al. Kelch-like 20 up-regulates the expression of hypoxia-inducible factor-2a through hypoxia- and von Hippel-Lindau tumor suppressor protein-independent regulatory mechanisms. Biochem Biophys Res Commun. 2011 Aug 24.[PMID: 21888897]
- Luo W, Hu H, Chang R, et al. Pyruvate Kinase M2 is a PHD3-Stimulated Coactivator for Hypoxia-Inducible Factor 1.Cell. 2011 May 27;145(5):732-44. [PMID: 21620138]
- Matak P, Deschemin JC, Peyssonnaux C, Vaulont S.Lack of iron-related phenotype in Sp6 intestinal knockout mice. Blood Cells Mol Dis. 2011 Apr 20.[PMID:21514188]
- Koh MY, Lemos R Jr, Liu XP, Powis G. The hypoxia associated factor (HAF) switches cells from HIF-1{alpha} to HIF-2{alpha} dependent signaling promoting stem cell characteristics, aggressive tumor growth and invasion. Cancer Res. 2011 Apr 21. [PMID: 21512133]
- Xie L, Collins JF. Transcriptional Regulation of the Menkes Copper ATPase (Atp7a) Gene by Hypoxia Inducible Factor (Hif2α) in Intestinal Epithelial Cells. Am J Physiol Cell Physiol. 2011 Feb 23 [PMID: 21346155]
- Nagao K, Oka K. HIF-2 directly activates CD82 gene expression in endothelial cells.Biochem Biophys Res Commun. 2011 Apr 1;407(1):260-5. [PMID: 21382346]
- Lange C, Heynen SR, Tanimoto N, et al. Normoxic activation of hypoxia inducible factors in photoreceptors provides transient protection against light induced retinal degeneration.Invest Ophthalmol Vis Sci. 2011 Mar 29. [PMID: 21447692]
- Chen C, Ma Q, Ma X et al. Association of Elevated HIF-2a Levels with Low Beclin 1 Expression and Poor Prognosis in Patients with Chondrosarcoma. Ann Surg Oncol. 2011 Feb 16. [PMID: 21327823]
- Hughes MD, Kapllani E, Alexander AE et al. HIF-2alpha downregulation in the absence of functional VHL is not sufficient for renal cell differentiation. Cancer Cell Int. 2007 Jun 28;7:13 [PMID: 17598890]
- Hu Z, Gulec S, Collins JF. Cross-species comparison of genomewide gene expression profiles reveals induction of hypoxia-inducible factor-responsive genes in iron-deprived intestinal epithelial cells.Am J Physiol Cell Physiol. 2010 Nov;299(5):C930-8. [PMID: 20702690]
- Neal CS, Michael MZ, Rawlings LH, et al. The VHL-dependent regulation of microRNAs in renal cancer.BMC Med. 2010 Oct 21;8(1):64. [PMID: 20964835]
- Lange C, Caprara C, Tanimoto N, et al. Retina-specific activation of a sustained hypoxia-like response leads to severe retinal degeneration and loss of vision. Neurobiol Dis. 2010 Sep 9. [PMID: 20817091]
- Minamishima YA, Moslehi J, Bardeesy N, et al. Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure. Blood. 2008 Mar 15;111(6):3236-44. [PMID: 18096761] (Mouse, WB)
- Conrad PW, Freeman TL, Beitner-Johnson D, et al. EPAS1 trans-activation during hypoxia requires p42/p44 MAPK. J Biol Chem. 1999 Nov 19;274(47):33709-13. [PMID: 10559262] (Mouse, WB)
- S. K. Martin, P. Diamond, S. A. Williams, et al. Hypoxia-inducible factor-2 is a novel regulator of aberrant CXCL12 expression in multiple myeloma plasma cells. Haematologica. 2010; 95 (5): 776-784. [PMID: 20015878]
- Henze AT, Riedel J, Diem T, et al. Prolyl hydroxylases 2 and 3 act in gliomas as protective negative feedback regulators of hypoxia-inducible factors. Cancer Res. 2010 Jan 1;70(1):357-66. [PMID: 20028863]
- Franovic A, Holterman CE, Payette J, et al. Human cancers converge at the HIF-2alpha oncogenic axis. Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21306-11. [PMID: 19955413]
- van Hagen M, Overmeer RM, Abolvardi SS, et al. RNF4 and VHL regulate the proteasomal degradation of SUMO-conjugated Hypoxia-Inducible Factor-2{alpha}. Nucleic Acids Res. 2009 Dec 21. [PMID: 20026589]
- Rey S, Lee K, Wang CJ, et al. Synergistic effect of HIF-1alpha gene therapy and HIF-1-activated bone marrow-derived angiogenic cells in a mouse model of limb ischemia. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20399-404. [PMID: 19948968]
- Lee K, Zhang H, Qian DZ, et al. Acriflavine inhibits HIF-1 dimerization, tumor growth, and vascularization. PNAS 2009;106(42):17910-17915. [PMID: 19805192] (Human, ChIP)
- Krieg AJ, Rankin EB, Chan D, et al. Regulation of the Histone Demethylase JMJD1A by HIF-1{alpha} Enhances Hypoxic Gene Expression and Tumor Growth. Mol Cell Biol 2009:MCB.00444-00409. [PMID: 19858293]
- Luo, W., Zhong, J., Chang, R., Hu, H., Pandey, A., Semenza, G.L. HSP70 and CHIP selectively mediate Ubiquitination and degradation of hypoxia-inducible factor (HIF)-1a but not HIF-2a J. Biol. Chem. jbc.M109.068577First Published on November 23, 2009, doi:10.1074/jbc.M109.068577
- Minamishima, Y.A., et al. A Feedback Loop Involving the Phd3 Prolyl Hydroxylase Tunes the Mammalian Hypoxic Response In Vivo. (2009) Mol. Cell. Biol. 29:5729-5741
- Rey, S., Lee, K., Wang, C.J., Gupta, K., Chen, S., McMillan, A., Bhise, N., Levchenko, A., Semenza, G. Synergistic effect of HIF-1a gene therapy and HIF-1-activated bone marrow-derived angiogenic cells in a mouse model of limb ischemia. PNAS 2009 106:20399-20404; published online before print December 1, 2009, doi:10.1073/pnas.0911921106
- Hansen SL, Trakooljul N, Liu HC, et al. Iron transporters are differentially regulated by dietary iron, and modifications are associated with changes in manganese metabolism in young pigs. J Nutr. 2009 Aug;139(8):1474-9. [PMID: 19535423]
- Tuller ER, Beavers CT, Lou JR, et al. Docosahexaenoic Acid Inhibits Superoxide Dismutase 1 Gene Transcription in Human Cancer Cells: The Involvement of Peroxisome Proliferator-Activated Receptor {alpha} Hypoxia-Inducible Factor-2{alpha} Signaling. Mol Pharmacol 2009;76(3):588-595. [pubmed: 19528198]
- Kim J, Bagchi IC, Bagchi MK. Signaling by Hypoxia-Inducible Factors Is Critical for Ovulation In Mice. Endocrinology 2009;150(7):3392-3400. [pubmed: 19325003]
- Schodel J, Klanke B, Weidemann A, et al. HIF-Prolyl Hydroxylases in the Rat Kidney: Physiologic Expression Patterns Regulation in Acute Kidney Injury. Am J Pathol 2009;174(5):1663-1674. [pubmed: 19349364] (Mouse, WB)
- Lomb DJ, Desouza LA, Franklin JL, et al. Prolyl Hydroxylase Inhibitors Depend on Extracellular Glucose Hypoxia-Inducible Factor (HIF)-2{alpha} to Inhibit Cell Death Caused by Nerve Growth Factor (NGF) Deprivation: Evidence that HIF-2{alpha} Has a Role in NGF-Promoted Survival of Sympathetic Neurons. Mol Pharmacol 2009;75(5):1198-1209. [pubmed: 19204094] (Mouse, WB)
- Luu V-D, Boysen G, Struckmann K, et al. Loss of VHL Hypoxia Provokes PAX2 Up-Regulation in Clear Cell Renal Cell Carcinoma. Clin Cancer Res 2009;15(10):3297-3304. [pubmed: 19401348]
- Fang H-Y, Hughes R, Murdoch C, et al. Hypoxia inducible factors 1 2 are important transcriptional effectors in primary macrophages experiencing hypoxia. Blood 2009:blood-2008-2012-195941. [pubmed: 19454749 ]
- Luu V-D, Boysen G, Struckmann K, et al. Loss of VHL Hypoxia Provokes PAX2 Up-Regulation in Clear Cell Renal Cell Carcinoma. Clin Cancer Res 2009:1078-0432.CCR-1008-2779 [pubmed: 19401348]
- Lee K, Qian DZ, Rey S, et al. Anthracycline chemotherapy inhibits HIF-1 transcriptional activity tumor-induced mobilization of circulating angiogenic cells. Proc Natl Acad Sci U S A. 2009;106(7):2353-2358. [pubmed: 19168635]
- Walter A, Etienne-Selloum N, Brasse D, et al. Red wine polyphenols prevent acceleration of neovascularisation by Ang II in the ischemic rat hindlimb. J Pharmacol Exp Ther. 2009 May;329(2):699-707. [pubmed: 19193929]
- Wacker I, Sachs M, Knaup K, et al. A Key Role for Activin B in Cellular Transformation after Loss of the von Hippel-Lindau (VHL) Tumor Suppressor. Mol Cell Biol 2009:MCB.01184-01107. [pubmed: 19158274]
- Regazzetti C, Peraldi P, Gremeaux T, et al. Hypoxia decreases insulin signaling pathways in adipocytes. Diabetes. 2009 Jan;58(1):95-103. Epub 2008 Nov 4.
- Sears JE, Hoppe G, Ebrahem Q, et al. Prolyl hydroxylase inhibition during hyperoxia prevents oxygen-induced retinopathy. Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19898-903. Epub 2008 Dec 4.
- Zhang H, Qian DZ, Tan YS, et al. Digoxin and other cardiac glycosides inhibit HIF-1alpha synthesis and block tumor growth. Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19579-86. Epub 2008 Nov 19.
- Trollmann R, Strasser K, Keller S, et al. Placental HIFs as markers of cerebral hypoxic distress in fetal mice. Am J Physiol Regul Integr Comp Physiol. 2008 Dec;295(6):R1973-81. Epub 2008 Oct 1.
- Koh MY, Darnay BG, Powis G. Hypoxia-associated factor, a novel E3-ubiquitin ligase, binds and ubiquitinates hypoxia-inducible factor 1alpha, leading to its oxygen-independent degradation. Mol Cell Biol. 2008 Dec;28(23):7081-95.
- Meyuhas R, Pikarsky E, Tavor E, et al. A Key role for cyclic AMP-responsive element binding protein in hypoxia-mediated activation of the angiogenesis factor CCN1 (CYR61) in Tumor cells. Mol Cancer Res. 2008 Sep;6(9):1397-409.
- Mahajan S, Dammai V, Hsu T, et al. Hypoxia-inducible factor-2alpha regulates the expression of TRAIL receptor DR5 in renal cancer cells. Carcinogenesis. 2008 Sep;29(9):1734-41. Epub 2008 Jun 9.
- Lu C-W, Lin S-C, Chen K-F, et al. Induction of pyruvate dehydrogenase kinase-3 by hypoxia-inducible factor-1 promotes metabolic switch and drug resistance. J Biol Chem. 2008 Oct 17;283(42):28106-14. Epub 2008 Aug 21.
- Puppo M, Battaglia F, Ottaviano C, et al. Topotecan inhibits vascular endothelial growth factor production and angiogenic activity induced by hypoxia in human neuroblastoma by targeting hypoxia-inducible factor-1alpha and -2alpha. Mol Cancer Ther. 2008 Jul;7(7):1974-84.
- Dutta D, Ray S, Vivian JL, et al. Activation of the VEGFR1 chromatin domain: an angiogenic signal-ETS1/HIF-2alpha regulatory axis. J Biol Chem. 2008 Sep 12;283(37):25404-13. Epub 2008 Jul 14.
- Lee K, Lynd JD, O';Reilly S, Kiupel M, McCormick JJ, LaPres JJ. The biphasic role of the hypoxia-inducible factor prolyl-4-hydroxylase, PHD2, in modulating tumor-forming potential. Mol Cancer Res. 2008 May;6(5):829-42.
- Arab A, Kuemmerer K, Wang J, Bode C, Hehrlein C. Oxygenated perfluorochemicals improve cell survival during reoxygenation by pacifying mitochondrial activity. J Pharmacol Exp Ther. 2008 May;325(2):417-24. Epub 2008 Feb 27.
- Yamashita T, Ohneda O, Sakiyama A, Iwata F, Ohneda K, Fujii-Kuriyama Y. The microenvironment for erythropoiesis is regulated by HIF-2alpha through VCAM-1 in endothelial cells. Blood. 2008 Aug 15;112(4):1482-92. Epub 2008 May 1.
- Korgaonkar SN, Feng X, Ross MD, et al. HIV-1 upregulates VEGF in podocytes. J Am Soc Nephrol. 2008 May;19(5):877-83.
- Takeda K, Aguila HL, Parikh NS, et al. Regulation of adult erythropoiesis by prolyl hydroxylase domain proteins. Blood. 2008 Mar 15;111(6):3229-35. Epub 2007 Dec 4.
- Ginouves A, Ilc K, Macias N, Pouyssegur J, Berra E. PHDs overactivation during chronic hypoxia "desensitizes" HIFalpha and protects cells from necrosis. Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4745-50. Epub 2008 Mar 17.
- Brown ST, Nurse CA. Induction of HIF-2alpha is dependent on mitochondrial O2 consumption in an O2-sensitive adrenomedullary chromaffin cell line. Am J Physiol Cell Physiol. 2008 Jun;294(6):C1305-12. Epub 2008 Mar 19.
- Zhou G, Dada LA, Chel NS, et al. Hypoxia-mediated Na-K-ATPase degradation requires von Hippel Lindau protein. FASEB J. 2008 May;22(5):1335-42. Epub 2007 Dec 11.
- Cheng G, Shao Z, Chaudhari B, Agrawal DK. Cheng G, Shao Z, Chaudhari B, Agrawal DK. Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2007 Nov;293(5):L1339-47. Epub 2007 Sep 14.
- Milkiewicz, M., J. L. Doyle, et al. (2007). HIF-1alpha and HIF-2alpha play a central role in stretch-induced but not shear-stress-induced angiogenesis in rat skeletal muscle. J Physiol. 2007 Sep 1;583(Pt 2):753-66. Epub 2007 Jul 12.
- Nagano, M., et al. Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007 Jul 1;110(1):151-60. Epub 2007 Mar 22.
- Zimmer, M., et al. Inhibition of hypoxia-inducible factor is sufficient for growth suppression of VHL-/- tumors. Mol Cancer Res. 2004 Feb;2(2):89-95.
- Zhang, H., et al. HIF-1 inhibits mitochondrial biogenesis and cellular respiration in VHL-deficient renal cell carcinoma by repression of C-MYC activity. Cancer Cell. 2007 May;11(5):407-20.
- Yuan, Y., et al. Cobalt inhibits the interaction between hypoxia-inducible factor-alpha and von Hippel-Lindau protein by direct binding to hypoxia-inducible factor-alpha. J Biol Chem. 2003 May 2;278(18):15911-6. Epub 2003 Feb 26.
- Xia, G., et al. Positive expression of HIF-2alpha/EPAS1 in invasive bladder cancer. Urology. 2002 May;59(5):774-8.
- Welsh, S., et al. Antitumor activity and pharmacodynamic properties of PX-478, an inhibitor of hypoxia-inducible factor-1alpha. Mol Cancer Ther. 2004 Mar;3(3):233-44.
- Uchida, T., et al. Prolonged hypoxia differentially regulates hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha expression in lung epithelial cells: implication of natural antisense HIF-1alpha. J Biol Chem. 2004 Apr 9;279(15):14871-8. Epub 2004 Jan 26.
- Toro, R., et al. Induction of T-type calcium channel gene expression by chronic hypoxia. J Biol Chem. 2003 Jun 20;278(25):22316-24. Epub 2003 Apr 4.
- Temes, E., et al. Activation of HIF-prolyl hydroxylases by R59949, an inhibitor of the diacylglycerol kinase. J Biol Chem. 2005 Jun 24;280(25):24238-44. Epub 2005 Apr 23.
- Tang, N.,et al. Loss of HIF-1alpha in endothelial cells disrupts a hypoxia-driven VEGF autocrine loop necessary for tumorigenesis. Cancer Cell. 2004 Nov;6 (5):485-95.
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Jump to: Conjugated Antibodies, Positive Controls, Antibody Packs, Lysates, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| HIF-2 alpha Antibody [Biotin] | NB100-122B | Fi, Hu, Mu, Rt | IHC-P, WB | (2) | (118) | | | | HIF-2 alpha Antibody [HRP] | NB100-122H | Fi, Hu, Mu, Rt | IHC-P, WB | (2) | (118) | | | | HIF-2 alpha Antibody [DyLight 488] | NB100-122G | Fi, Hu, Mu, Rt | IHC, IHC-P, WB | | (118) | | | | HIF-2 alpha Antibody [DyLight 550] | NB100-122R | Fi, Hu, Mu, Rt | IHC, IHC-P, WB | | (118) | | | | HIF-2 alpha Antibody [DyLight 650] | NB100-122C | Fi, Hu, Mu, Rt | IHC, IHC-P, WB | | (118) | | |
Positive ControlsAntibody Packs (1)Lysates (3)Primary Antibodies (15)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| HIF-2 alpha Antibody (ep190b) | NB100-132 | Hu, Mu, Rt | ELISA, FACS, IHC-P, WB | (2) | (45) | | | | HIF-2 alpha Antibody | NBP1-33277 | Hu | IHC-P, WB | (3) | | | | | HIF-2 alpha Antibody | H00002034-D01P | Hu | ELISA, WB | (1) | | | | | HIF-2 alpha Antibody | H00002034-B02P | Hu | Func, WB | | | | | | HIF-2 alpha Antibody [HRP] | NB100-122H | Fi, Hu, Mu, Rt | IHC-P, WB | (2) | (118) | | | | ... and 10 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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