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HIF-2 alpha Antibody (ep190b) (NB100-132) (0.1 ml)

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	Catalog Number	NB100-132 (Sample Size Available)
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(see alternate names)

anti-BHLHE73 antibody, anti-Member of PAS protein 2 antibody, anti-hypoxia-inducible factor 2 alpha antibody, anti-Hypoxia-inducible factor 2-alpha antibody, anti-HIF-1alpha-like factor antibody, anti-Class E basic helix-loop-helix protein 73 antibody, anti-endothelial PAS domain-containing protein 1 antibody, anti-Basic-helix-loop-helix-PAS protein MOP2 antibody, anti-bHLHe73ECYT4 antibody, anti-PASD2PAS domain-containing protein 2 antibody, anti-HLFHIF-1-alpha-like factor antibody, anti-MOP2HIF2-alpha antibody, anti-HIF2AHIF-2-alpha antibody, anti-EPAS-1 antibody, anti-endothelial PAS domain protein 1 antibody

HIF-2 alpha Antibody (ep190b) Summary:

Species:	Hu, Mu, Rt	Gene:	EPAS1
Applications:	WB, ELISA, FACS, IHC-P	Purity:	Protein G purified
Clonality:	Monoclonal	Host:	Mouse
Specificity:	This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Publications:	NB100-132 has been mentioned in at least 50 Publications

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HIF-2 alpha Antibody (ep190b) Details:

Clone:	ep190b
Isotype:	IgG1
Immunogen:	Human HIF-2 alpha amino acids 535-631.

Species Reactivity:

NB100-132 reacts with human and rat HIF-2 alpha. Ability to use in mouse is mixed with some positive and some negative results. Other species have not been tested.

Applications:

Uses:	Western Blot (recognizes a band at ~118 kDa representing HIF-2 alpha) Immunohistochemistry: Has been used on paraffin-embedded sections an approximate 1:150-1:300 range
Dilutions:	ELISA, Flow Cytometry 1.0 ug, Immunohistochemistry-Paraffin 1:150-1:300, Western Blot 1:500
Unit Size:	0.1 ml (also available: 0.025 ml (sample size))
Concentration:	1.0 mg/ml
Positive Controls:	2 Positive Controls
Conjugated Antibodies:	5 Conjugated Antibodies

Packaging:

Storage:	Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Buffer:	PBS
Preservative:	0.1% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: EPAS1

Entrez:	2034 (Human)
	13819 (Mouse)
	29452 (Rat)
Uniprot:	Q99814 (Human)

Related Genes:

Background:

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1 alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.

Images (2)


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Reviews (1)

Wei-Chien Yuan on 12/14/2011

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HIF-2 alpha Antibody (ep190b) Images (2)

	Western Blot: HIF-2 alpha antibody [NB100-132] - HIF-2 alpha detected in hypoxic human lysate using NB 100-132. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.

	Immunohistochemistry: HIF-2 alpha antibody [NB100-132] - Staining of heart, cardiac myocytes using NB100-132.

Reviews (1) Have you used this product? Review this product to earn Novus Rewards.

Reviewed by:
Wei-Chien Yuan 12/14/2011

Application:	Western Blot
Sample Tested:	Human
Species:	Human
Sample Pretreated:	Hypoxic

Blocking Buffer:

1% Milk

Dilution Ratio:	1:500
Incubation Time:	O/N
Incubation Temperature:	4 Celsius

Dilution Ratio:

1:2000

Method:

ECL

Publications (50)

HIF-2 alpha Antibody (ep190b) Product Specific:

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Conley SJ, Gheordunescu E, Kakarala P, Newman B, Korkaya H, Heath AN, Clouthier SG, Wicha MS. Antiangiogenic agents increase breast cancer stem cells via the generation of tumor hypoxia. Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):2784-9. [PMID: 22308314]
Cartee TV, White KJ, Newton-West M, Swerlick RA. Hypoxia and hypoxia mimetics inhibit TNF-dependent VCAM1 induction in the 5A32 endothelial cell line via a hypoxia inducible factor dependent mechanism. J Dermatol Sci. 2012 Feb;65(2):86-94. [PMID: 22093255]
Bohonowych JES, Peng S, Gopal U, et al. Comparative analysis of novel and conventional Hsp90 inhibitors on HIF activity and angiogenic potential in clear cell renal cell carcinoma: implications for clinical evaluation. BMC Cancer. 2011 Dec 15.11(1):520. [PMID: 22172030]
Husted RF, Lu H, Sigmund RD, Stokes JB. Oxygen regulation of the epithelial Na channel (ENaC) in the collecting duct. Am J Physiol Renal Physiol. 2010 Dec 1. [PMID: 21123494]
Bangiyeva V, Rosenbloom A, Alexander AE, et al. Differences in regulation of tight junctions and cell morphology between VHL mutations from disease subtypes. BMC Cancer. 2009 Jul 14;9:229. [PMID: 19602254]
Harvey, A. J., et al. Regulation of gene expression in bovine blastocysts in response to oxygen and the iron chelator desferrioxamine. Biol Reprod. 2007 Jul;77(1):93-101. [PMID: 17329595] (Human, ICC/IF)
JA Walker. EXPRESSION OF HUMAN PAPILLOMAVIRUS TYPE 16 E7 IS SUFFICIENT TO SIGNIFICANTLY INCREASE EXPRESSSION OF ANGIOGENIC FACTORS BUT IS NOT SUFFICIENT TO INDUCE ENDOTHELIAL CELL MIGRATION. Indiana University, in partial fulfillment of the requirements for the degree of Doctor of Philosophy.August 2010. link: https://scholarworks.iupui.edu/bitstream/handle/1805/2291/Joanna%20W.%20Thesis.pdf?sequence=1
S. K. Martin, P. Diamond, S. A. Williams, et al. Hypoxia-inducible factor-2 is a novel regulator of aberrant CXCL12 expression in multiple myeloma plasma cells. Haematologica. 2010; 95 (5): 776-784. [PMID: 20015878]
Beasley NJ, Leek R, Alam M, et al. Hypoxia-inducible factors HIF-1alpha and HIF-2alpha in head and neck cancer: relationship to tumor biology and treatment outcome in surgically resected patients. Cancer Res. 2002 May 1;62(9):2493-7. [PMID: 11980639] (IHC-P, Human)
Kim Y, Lin Q, Zelterman D, et al. Hypoxia-regulated delta-like 1 homologue enhances cancer cell stemness and tumorigenicity. Cancer Res. 2009 Dec 15;69(24):9271-80. [PMID: 19934310]
Noguera R, Fredlund E, Piqueras M, et al. HIF-1alpha and HIF-2alpha are differentially regulated in vivo in neuroblastoma: high HIF-1alpha correlates negatively to advanced clinical stage and tumor vascularization. Clin Cancer Res. 2009 Dec 1;15(23):7130-6. [PMID: 19903792]
Lee DW, Rajagopalan S, Siddiq A, et al. Inhibition of Prolyl Hydroxylase Protects against 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Neurotoxicity: MODEL FOR THE POTENTIAL INVOLVEMENT OF THE HYPOXIA-INDUCIBLE FACTOR PATHWAY IN PARKINSON DISEASE. J Biol Chem 2009;284(42):29065-29076. [PMID: 19679656]
Abbruzzetti S, Faggiano S, Bruno S, et al. Ligand migration through the internal hydrophobic cavities in human neuroglobin. PNAS 2009:0905433106. [PMID: 19850865]
Bastian Eul, Frank Rose,Stefanie Krick. Impact of HIF-1#945; HIF-2#945; on proliferation migration of human pulmonary artery fibroblasts in hypoxia. The FASEB Journal express article 10.1096/fj.05-4104fje.
Ndubuizu OI, Chavez JC, LaManna JC. Increased prolyl 4-hydroxylase expression differential regulation of hypoxia-inducible factors in the aged rat brain. Am J Physiol Regulatory Integrative Comp Physiol 2009;297(1):R158-165. [pubmed: 19420289]
Wiesener MS, Turley H, Allen WE, Willam C, Eckardt KU, Talks KL, Wood SM, Gatter KC, Harris AL, Pugh CW, Ratcliffe PJ, Maxwell PH. Induction of endothelial PAS domain protein-1 by hypoxia: characterization and comparison with hypoxia-inducible factor-1alpha. Blood. 1998 Oct 1;92(7):2260-8.
Tuller ER, Beavers CT, Lou JR, et al. Docosahexaenoic acid inhibits superoxide dismutase 1 gene transcription in human cancer cells: the involvement of PPAR{alpha} HIF-2{alpha} signaling. Mol Pharmacol 2009:mol.109.057430. [pubmed: 19528198]
Walter A, Etienne-Selloum N, Brasse D, et al. Red Wine Polyphenols Prevent Acceleration of Neovascularization by Angiotensin II in the Ischemic Rat Hindlimb. J Pharmacol Exp Ther 2009;329(2):699-707. [pubmed: 19193929]
Burkitt K, Chun SY, Dang DT, et al. Targeting both HIF-1 HIF-2 in human colon cancer cells improves tumor response to sunitinib treatment. Mol Cancer Ther 2009;8(5):1148-1156. [pubmed: 19435875]
Wacker I, Sachs M, Knaup K, Wiesener M, Weiske J, Huber O, Akçetin Z, Behrens J. Key role for activin B in cellular transformation after loss of the von Hippel-Lindau tumor suppressor. Mol Cell Biol. 2009 Apr;29(7):1707-18. Epub 2009 Jan 21.
Chintalapudi MR, Markiewicz M, Kose N, Dammai V, Champion KJ, Hoda RS, Trojanowska M, Hsu T. Cyr61/CCN1 and CTGF/CCN2 mediate the proangiogenic activity of VHL-mutant renal carcinoma cells. Carcinogenesis. 2008 Apr;29(4):696-703. Epub 2008 Jan 22.
Kubo T, Sugita T, Shimose S, Matsuo T, Arihiro K, Ochi M. Expression of hypoxia-inducible factor-1{alpha} its relationship to tumour angiogenesis cell proliferation in cartilage tumours. J Bone Joint Surg Br. March 1, 2008;90-B(3):364-70. (WB)
Martín-Puig S, Temes E, Olmos G, Jones DR, Aragonés J, Landázuri MO. Role of iron (II)-2-oxoglutarate-dependent dioxygenases in the generation of hypoxia-induced phosphatidic acid through HIF-1/2 and von Hippel-Lindau-independent mechanisms. J Biol Chem. 2004 Mar 5;279(10):9504-11. Epub 2003 Dec 17.
Giatromanolaki A, Koukourakis MI, Sivridis E, et al. Expression of hypoxia-inducible carbonic anhydrase-9 relates to angiogenic pathways and independently to poor outcome in non-small cell lung cancer. Cancer Res. 2001 Nov 1;61(21):7992-8. [PMID: 11691824] (IHC-P, Human)
Talks, K.L. Turley, H. Amer. J. of Pathology. 157: 411-421, 2000. (WB/IHC)
Blancher, C., et al. Cancer Research. 60: 7106-7113, 2000. (WB/IHC)
Koukourakis MI, Giatromanolaki A, Skarlatos J, et al. Hypoxia inducible factor (HIF-1a and HIF-2a) expression in early esophageal cancer and response to photodynamic therapy and radiotherapy. Cancer Res. 2001 Mar 1;61(5):1830-2. [PMID: 11280732] (IHC-P, Human)
Sutphin, P.D., et al. Tageting the Loss of the von Hippel-Lindau Tumor Suppressor Gene in Renal Cell Carcinoma Cells. Cancer Res. 2007 67: 5896-5905.
Carroll, P. A., et al. Latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells activates hypoxia-induced factors. J Virol. 2006 Nov;80(21):10802-12. [PMID: 16956952] (Human/IHC-P)
Koizume, Shiro., et al. Activation of Cancer cell Migration Invasion by Ectopic Synthesis of Coagulation Factor VII. Cancer Res. 2006 Oct 1;66(19):9453-60. [pubmed: 17018600] (ChIP)
Zimmer, M., et al. Inhibition of Hypoxia-Inducible Factor Is Sufficient for Growth Supression of VHL-/- Tumors. Molecular Cancer Research. 2: 89-95, 2004. [pubmed: 14985465] (WB)
Zheng X, Ruas JL, Cao R, Salomons FA, Cao Y, Poellinger L, Pereira T. Cell-type-specific regulation of degradation of hypoxia-inducible factor 1 alpha: role of subcellular compartmentalization. Mol Cell Biol. 2006 Jun;26(12):4628-41.
Yoshimura H, Dhar DK, Kohno H, Kubota H, Fujii T, Ueda S, Kinugasa S, Tachibana M, Nagasue N. Prognostic impact of hypoxia-inducible factors 1alpha and 2alpha in colorectal cancer patients: correlation with tumor angiogenesis and cyclooxygenase-2 expression. Clin Cancer Res. 2004 Dec 15;10(24):8554-60.
Yan Q, Bartz S, Mao M, Li L, Kaelin WG Jr. The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Mol Cell Biol. 2007 Mar;27(6):2092-102. Epub 2007 Jan 12.
Xia G, et al. (2002) Positive expression of HIF-2alpha/ EPAS1 in invasive bladder cancer. Urology. 2002 May;59(5):774-8. [pubmed: 11992927]
Tissot van Patot MC, Bendrick-Peart J, Beckey VE, Serkova N, Zwerdlinger L. Greater vascularity, lowered HIF-1/DNA binding, and elevated GSH as markers of adaptation to in vivo chronic hypoxia. Am J Physiol Lung Cell Mol Physiol. 2004 Sep;287(3):L525-32. Epub 2004 May 7.
Shinojima, Toshiaki, et al. Cancer Biology: Renal cancer cells lacking hypoxia inducible factor (HIF)-1a expression maintain vascular endothelial growth factor expression through HIF-2a. Carcinogenesis. 2007 Mar;28(3):529-36. [pubmed: 16920734] (WB)
Rathmell WK, Hickey MM, Bezman NA, Chmielecki CA, Carraway NC, Simon MC. In vitro and in vivo models analyzing von Hippel-Lindau disease-specific mutations. Cancer Res. 2004 Dec 1;64(23):8595-603.
Pan Y, Oprysko PR, Asham AM, Koch CJ, Simon MC. p53 cannot be induced by hypoxia alone but responds to the hypoxic microenvironment. Oncogene. 2004 Jun 24;23(29):4975-83.
Nakamura E, Abreu-e-Lima P, Awakura Y, Inoue T, Kamoto T, Ogawa O, Kotani H, Manabe T, Zhang GJ, Kondo K, Nosé V, Kaelin WG Jr. Clusterin is a secreted marker for a hypoxia-inducible factor-independent function of the von Hippel-Lindau tumor suppressor protein. Am J Pathol. 2006 Feb;168(2):574-84.
Mohan, S., et al. von Hippel-Linau protein complex is regulated by cell density. Oncogene. 22: 5270-5280, 2003. [pubmed: 12917628] (WB)
Mekhail, K., et al. HIF activation by pH-dependent nucleolar sequestration of VHL. Nature Cell Biology. 6: 642-647, 2004. [pubmed: 15181450]
Li, L., et al. Evaluating Hypoxia-Inducible Factors-1alpha as a Cancer Therapeutic Target via Inducible RNA Interference In vivo. American Association for Cancer Research 65, 7249-7258, 2005. [pubmed: 16103076] (WB/IHC, Human)
Koizume S, Jin MS, Miyagi E, Hirahara F, Nakamura Y, Piao JH, Asai A, Yoshida A, Tsuchiya E, Ruf W, Miyagi Y. Activation of cancer cell migration and invasion by ectopic synthesis of coagulation factor VII. Cancer Res. 2006 Oct 1;66(19):9453-60.
Koike, T., et al. Hypoxia induces adhesion molecules on cancer cells: A missing link between Warburg effect induction of selectin-lig carbohydrates. PNAS. 101:8132-8137, 2004. [pubmed: 15141079]
Harvey AJ, Kind KL, Pantaleon M, Armstrong DT, Thompson JG. Oxygen-regulated gene expression in bovine blastocysts. Biol Reprod. 2004 Oct;71(4):1108-19. Epub 2004 May 26.
Grabmaier, K., et al. Strict regulation of CAIXG250/MN by HIF-1 alpha in clear cell renal cell carcinoma. Oncogene. 2004 Jul 22;23(33):5624-31. [PMID: 15184875] (Human/EMSA)
Bernhardt WM, Wiesener MS, Weidemann A, et al. Involvement of hypoxia-inducible transcription factors in polycystic kidney disease. Am J Pathol. 2007 Mar;170(3):830-42. [PMID: 17322369] (RAT/IHC-P)

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Jump to: Conjugated Antibodies, Positive Controls, Antibody Packs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Conjugated Antibodies

Name	Catalog#	Reactivity	Applications	Images	Publications
HIF-2 alpha Antibody (ep190b) [DyLight 550]	NB100-132R	Hu, Mu, Rt	FACS, IHC-P, WB		(33)
HIF-2 alpha Antibody (ep190b) [DyLight 650]	NB100-132C	Hu, Mu, Rt	FACS, IHC-P, WB		(32)
HIF-2 alpha Antibody (ep190b) [HRP]	NB100-132H	Hu, Mu, Rt	IHC-P, WB	(2)	(31)
HIF-2 alpha Antibody (ep190b) [DyLight 488]	NB100-132G	Hu, Mu, Rt	FACS, IHC-P, WB		(32)
HIF-2 alpha Antibody (ep190b) [Biotin]	NB100-132B	Hu, Mu, Rt	IHC-P, WB	(2)	(29)

Positive Controls

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
RCC4 Lysate	NBP1-30412		WB	(2)
HIF-2 alpha Lysate	NBL1-10286		WB	(1)	(1)

Antibody Packs (1)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
HIF-2 alpha Antibody Pack 2	NB100-902HIF2	Hu, Mu, Rt	ELISA, FACS, ICC, IHC, IHC-Fr, IHC-P, WB	(4)	(13)

Lysates (3)

Name	Catalog#	Applications	Images	Publications
HIF-2 alpha Lysate	NBL1-10286	WB	(1)	(1)
HIF-2 alpha Lysate	H00002034-T01	WB
HIF-2 alpha Lysate	H00002034-T02	WB

Peptides and Proteins

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
HIF-2 alpha Recombinant Protein	H00002034-P03	Hu	ELISA, WB

Primary Antibodies (16)

Name	Catalog#	Reactivity	Applications	Images	Publications
HIF-2 alpha Antibody	NB100-122	Fi, Hu, Mu, Rt	ChIP, ELISA, ICC, IF, IHC-P, IP, WB	(4)	(175)
HIF-2 alpha Antibody	NBP1-33277	Hu	IHC-P, WB	(3)	(1)
HIF-2 alpha Antibody	H00002034-D01P	Hu	ELISA, WB	(1)
HIF-2 alpha Antibody	H00002034-B02P	Hu	Func, WB
HIF-2 alpha Antibody (1G2)	H00002034-M08	Hu	ELISA, WB
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RNAi (1)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
HIF-2 alpha RNAi	H00002034-R01	Hu		(1)	(4)

Secondary Antibodies

Name	Catalog#	Reactivity	Applications	Publications
Mouse IgG Antibody [Biotin]	NB720-B	Mu	ELISA, IHC-P, WB
Mouse IgG Antibody [HRP]	NB720-H	Mu	ELISA, ICC, IHC-P, WB
Mouse IgG Antibody [Alkaline Phosphatase]	NB720-AP	Mu	ELISA, ICC, IHC-P, WB	(1)
Mouse IgG Antibody [FITC]	NB720-F	Mu	ICC, IHC-P	(2)
Mouse IgG Antibody	NB120-7056	Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-)	ELISA, IHC-P, IP, WB
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HIF-2 alpha in Context

Research Areas related to HIF-2 alpha (6):