HIF-2 alpha Antibody Pack 2 (NB100-902HIF2)

HIF-2 alpha Antibody Pack 2 (NB100-902HIF2) (2 Vials)

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	Catalog Number	NB100-902HIF2
	Size(s)	2 Vials	Price:
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HIF-2 alpha Antibody Pack 2 Summary:

Species:	Hu, Mu, Rt	Gene:	EPAS1
Applications:	WB, ELISA, FACS, ICC, IHC, IHC-Fr, IHC-P	Purity:
Clonality:		Host:	Mouse
Specificity:	These antibodies are specific for HIF-2alpha.
Publications:	NB100-902HIF2 has been mentioned in at least 13 Publications

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HIF-2 alpha Antibody Pack 2 Details:

Immunogen:	NB 100-122 = Proprietary peptide derived from the mouse/human HIF-2a. NB 100-132 = Human HIF-2a amino acids 535-631.
Kit Components

Species Reactivity:

NB 100-122 recognizes human, mouse, and rat HIF-2a. Other species have not been tested.NB 100-132 reacts with human and rat HIF-2 alpha. Results in mouse have been mixed (some + and some -). Other species have not been tested.

Applications:

Uses:	By Western blot, these antibodies recognize a band ~118 kDa, representing HIF-2a in induced tissues and cells. The monoclonal has been used for immunohistochemistry. The polyclonal has also been used, but success has varied. Nuclear extracts should be used for Western analysis, if possible.
Dilutions:	ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Unit Size:	2 Vials
Concentration:	Concentration of individual antibodies may be found on the vial label.
Positive Controls:	1 Positive Controls

Notes:

1 vial each of: NB100-122SS and NB100-132SS

Packaging:

Storage:	Storage is content dependent.
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: EPAS1

Related Genes:

Background:

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.

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