HIF-1 alpha Antibody Pack 4 vials (NB100-901IHC)

HIF-1 alpha Antibody Pack 4 vials (NB100-901IHC) ( 4 vials)

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	Catalog Number	NB100-901IHC
	Size(s)	4 vials	Price:
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HIF-1 alpha Antibody Pack 4 vials Summary:

Species:	Hu, Mu, Rt	Gene:	HIF1A
Applications:	WB, FACS, ICC, IF, IHC, IHC-Fr, IHC-P, IP	Purity:
Clonality:		Host:
Specificity:	These antibodies are specific for HIF-1alpha.
Publications:	NB100-901IHC has been mentioned in at least 5 Publications

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HIF-1 alpha Antibody Pack 4 vials Details:

Immunogen:	NB 100-131: Human HIF-1 alpha amino acids 329-530. NB100-123: Fusion protein containing amino acids 432-528 of human HIF-1alpha. NB100-449: peptide between residues 775 and the C-terminus (residue 826) of human HIF-1 alpha. NB100-479: A fusion protein
Kit Components

Applications:

Uses:	Immunohistochemical staining of paraffin-embedded and frozen sections (only NB 100-131 has been used on frozen sections). Antigen retrieval is strongly recommended. With the exception of NB100-449 and NB100-479 (which are also recommended for Western Blot), these antibodies work for Western blot analysis but are not specifically recommended for this application. A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background.
Dilutions:	Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot
Unit Size:	4 vials
Concentration:	Concentration of individual antibodies may be found on the vial label.

Notes:

1 vial each of: NB100-479 (0.025mL), NB100-449 (0.025mL), NB100-123 (0.05mL) and NB100-131 (0.05mL).

Packaging:

Storage:	Store at 4°C. Do not freeze.
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: HIF1A

Related Genes:

Background:

Our HIF-1 alpha sample pack provides a convenient way to determine the optimal HIF-1 alpha antibody for your specific species and tissues. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein that activates gene transcription in response to reduced cellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, heme oxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are induced by hypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulate hypoxia-inducible factors may be related to tumor-related angiogenesis in certain cancers.

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Working with HIF-1 alpha

HIF-1 alpha Antibody Pack 4 vials Images (15)

	Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-901IHC] - Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using NB100-123.

$Western Blot: HIF-1 alpha Antibody [NB100-901IHC] - On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB 100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.$	Western Blot: HIF-1 alpha Antibody [NB100-901IHC] - On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB 100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.

	Western Blot: HIF-1 alpha Antibody [NB100-901IHC] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic) NB100-449.

	Western Blot: HIF-1 alpha antibody [NB100-901IHC] - Detection of mouse HIF1-alpha on hypoxia treated MEFs using NB 100-449.

	Immunohistochemistry: HIF-1 alpha antibody [NB100-901IHC] - Staining of HIF-1 alpha in human kidney using NB100-123.

	Immunohistochemistry: HIF-1 alpha Antibody [NB100-901IHC] - Staining of mouse brain, neurons using NB100-449.

	Immunohistochemistry: HIF-1 alpha Antibody [NB100-901IHC] - Staining of placenta, villi using.

	Immunohistochemistry: HIF-1 alpha Antibody [NB100-901IHC] - Staining in hypoxia-induced human placenta.

	Immunohistochemistry: HIF-1 alpha Antibody [NB100-901IHC] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.

	Immunofluorescence: HIF-1 alpha Antibody [NB100-901IHC] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. Blue dye is DAPI nuclear staining. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.

	Immunofluorescence: HIF-1 alpha Antibody [NB100-901IHC] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. 100X magnification. Blue dye is DAPI nuclear staining. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.

	Immunofluorescence: HIF-1 alpha Antibody [NB100-901IHC] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.

	Immunofluorescence: HIF-1 alpha Antibody [NB100-901IHC] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.

	Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-901IHC] -- Immunoprecipitation of HIF-1alpha using NB100-123. Heavy and light chains are also detected.

	Flow Cytometry: HIF-1 alpha Antibody [NB100-901IHC] - Flow Cytometric Analysis of HIF1-alpha in CoCl2 Treated vs. Untreated HeLa Cells. Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. One million cells were stained with 0.125ug of NB100-449 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Reduntreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.

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Publications (5)

HIF-1 alpha Antibody Pack 4 vials Product Specific:

Lee, S., Kim, S.J., Kim, D.J., et al. The Clinical Significanse of the Hypoxia Inducible Factor-1 C4d in Implantation Renal Biopsies Post-Transplant Biopsies. 22nd International Congress of the Transplantation Society. August 10, 2008. Sidney Australia.
Hill, P, et al. Inhibition of hypoxia inducible factor hydroxylases protects against renal ischemia-reperfusion injury. J Am Soc Nephrol. 2008 Jan;19(1):39-46.

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