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HIF-1 alpha Antibody (H1alpha67) 0.1ml

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  • HIF-1 alpha Primary Antibodies
  • All HIF-1 alpha Products
  • 13 Reviews  
  • 323 Publications
  • Alternate Names
     
  • Pathways & Diseases
  • Bioinformatics Tool

Ordering Information

Catalog Number  NB100-105
Sample Size Available
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Jump to Information on HIF-1 alpha Antibody (NB100-105)

HIF-1 alpha Antibody (H1alpha67) Summary
 
Species Human, Mouse, Rat, Bovine, Ferret, Porcine, Primate, Rabbit, Sheep
Tested Applications WB, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality Monoclonal
Host Mouse
Gene HIF1A
Purity Protein G purified
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HIF-1 alpha Antibody (H1alpha67) Details
 
Clone H1alpha67
Isotype IgG2b
Immunogen Fusion protein containing amino acids 432-528 of human HIF-1 alpha. [UniProt# Q16665]
Localization HIF-1 is a nuclear protein.
Species Reactivity
 
Human, monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret.
Reviewed Species Read 13 reviews rated 4.2  using NB100-105 in the following species:
 
Publications Read 143 publications using NB100-105 in the following species:
 
Applications/Dilutions
 
Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation 1:10-1:500
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:25-1:100
  • Immunohistochemistry-Frozen 1:25 - 1:100
  • Immunohistochemistry-Paraffin 1:25 - 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes

This HIF-1 alpha (H1alpha67) antibody is useful for Chromatin Immunoprecipitation (PMID 21871655). Gel Super Shift Assays, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band can be seen at 120 kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended. This antibody has been used to immunoprecipitate human HIF-1 alpha. For ChIP, refer to research papers with PubMed ID 16204079 and 21871655. This product has been cited for Gel Super Shift Assays in PubMed ID 22411794.
Molecular Weight

120 kDa
Positive Controls

2 Positive Controls
Directly Conjugated
Formats
Biotin Labeled NB100-105B
DyLight 550 Labeled NB100-105R
HRP Labeled NB100-105H
DyLight 488 Labeled NB100-105G
DyLight 650 Labeled NB100-105C
Learn about Custom Antibody Labeling
Reviewed Applications
Read 13 reviews rated 4.2  using
NB100-105 in the following applications:
Publications
Read 145 publications using
NB100-105 in the following applications:
Product PDFs
  Download PDF Datasheet
  Request CofA
  Download Protocol 1
  Download Protocol 2
  Download Protocol 3
  Request MSDS
Reviews
Average Rating: Very Good 
Recent Reviews

Test

  • {"id":1641,"rating":4,"ratetext":"4","title":"WB Tested In Mouse","date":"12/09/2011"}
  • {"id":1696,"rating":4,"ratetext":"4","title":"WB Tested In Rat","date":"12/14/2011"}
  • {"id":2476,"rating":4,"ratetext":"4","title":"WB Tested In Hu","date":"05/11/2012"}
  • {"id":2576,"rating":5,"ratetext":"5","title":"WB Tested In Mo","date":"05/15/2012"}
  • {"id":3436,"rating":5,"ratetext":"5","title":"WB Tested In Hu","date":"08/22/2012"}
  • {"id":3451,"rating":5,"ratetext":"5","title":"ICC/IF Tested In Ra","date":"08/22/2012"}
  • {"id":3486,"rating":5,"ratetext":"5","title":"WB Tested In Hu","date":"08/23/2012"}

Read all 13 Reviews
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Publications
HIF-1 alpha Antibody (NB100-105) has been mentioned in at least 323 publications.
Earn rewards if you have published using HIF-1 alpha Antibody (NB100-105).
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Protocols and FAQs
Controls & Support Products
View all related HIF-1 alpha Controls and Support Products.
Research Tools
View all HIF-1 alpha Research Tools.
Notes
 
There are reports that this antibody does not detect mouse in IHC-paraffin embedded tissue. It does work to detect mouse protein in Western blot.
Packaging, Storage & Formulations
 
Storage Store at 4C. Do not freeze.
Buffer PBS
Unit Size 0.1ml (also available in 0.025ml (sample size))
Concentration 4 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt.
Bioinformatics
 
Gene Symbol HIF1A
Entrez
OMIM
Uniprot
Alternate Names for HIF-1 alpha
 
  • ARNT-interacting protein
  • Basic-helix-loop-helix-PAS protein MOP1
  • BHLHE78
  • Class E basic helix-loop-helix protein 78
  • HIF-1-alpha
  • HIF1-alpha
  • hypoxia inducible factor 1
  • alpha subunit (basic h
  • hypoxia-inducible factor 1-alpha
  • Member of PAS protein 1
  • member of PAS superfamily 1
  • MOP1HIF1-ALPHA
  • PAS domain-containing protein 8
  • PASD8alpha subunit (basic helix-loop-helix transcr
Related Products by Gene
 
VEGFA, SETD2, ARNT, EPO, VHL, EPAS1, VON HIPPEL-LINDAU TUMOR SUPPRESSOR, AKT1, HIF-2ALPHA, SLC2A1, CA9, TP53, MAPK3, MAPK1, MTOR, HIF-1 ALPHA, NOS2, TIMP1, TNF, EGLN1
Background
 
HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.