HIF-1 alpha Antibody (H1alpha67) 0.1 ml

HIF-1 alpha NB100-105

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  • HIF-1 alpha Primary Antibodies
  • All HIF-1 alpha Products
  • 21 reviews  
  • 442 Publications
  • Alternate Names
     

Ordering Information: NB100-105

Catalog NumberNB100-105
  • FormUnconjugated
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HIF-1 alpha Antibody (H1alpha67) Summary

 
Species Human, Mouse, Rat, Bovine, Ferret, Porcine, Primate, Rabbit, Sheep, Xenopus, Yeast
Tested Applications WB, ChIP, ELISA, FLOW, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality Monoclonal
Host Mouse
Gene HIF1A
Purity Protein G purified
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HIF-1 alpha Antibody (H1alpha67) Details

 
Clone H1alpha67
Isotype IgG2b
Immunogen Fusion protein containing amino acids 432-528 of human HIF-1 alpha. [UniProt# Q16665]
Localization HIF-1 is a nuclear protein.

Species Reactivity

 
Human, monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret. Reactivity with Candida albicans reported by a customer review. Reactivity with Xenopus reported in the scientific literature (PMID: 18303027).
Reviewed Species Read 21 rated 4.3  using NB100-105 in the following species:
 
Publications Read Publications using NB100-105 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA
  • Flow Cytometry
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:20-1:50
  • Immunohistochemistry-Frozen 1:20-1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Immunoprecipitation 1:10-1:500
Application Notes

This HIF-1 alpha (H1alpha67) antibody is useful for Chromatin Immunoprecipitation (PMID: 21871655), ELISA (PMID: 20042684), Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band can be seen at 120 kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended. This antibody has been used to immunoprecipitate human HIF-1 alpha. For ChIP, refer to research papers with PubMed ID 16204079 and 21871655. This product has been cited for Gel Super Shift Assays in PubMed ID 22411794.

Molecular Weight

120 kDa

Positive Controls

2 Positive Controls

Other Available
Formats
Alexa Fluor (R) 405 Labeled NB100-105AF405
Alexa Fluor (R) 488 Labeled NB100-105AF488
Alexa Fluor (R) 647 Labeled NB100-105AF647
Alexa Fluor (R) 700 Labeled NB100-105AF700
Biotin Labeled NB100-105B
DyLight 405 Labeled NB100-105V
DyLight 488 Labeled NB100-105G
DyLight 550 Labeled NB100-105R
DyLight 650 Labeled NB100-105C
FITC Labeled NB100-105F
HRP Labeled NB100-105H
Learn about Custom Antibody Labeling
Reviewed Applications
Read 17 reviews rated 4.4  using
NB100-105 in the following applications:
Publications
Read publications using
NB100-105 in the following applications:

Product PDFs

Reviews

Average Rating: 4.3 
Recent Reviews

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Publications

HIF-1 alpha Antibody (NB100-105) has been mentioned in at least 442 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all HIF-1 alpha Research Tools.

Notes

 
There are reports that this antibody does not detect mouse in IHC-paraffin embedded tissue. It does work to detect mouse protein in Western blot.

Packaging, Storage & Formulations

 
Storage Store at 4C. Do not freeze.
Buffer PBS
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 1.0 mg/ml
Preservative 0.02% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol HIF1A
Entrez
OMIM
Uniprot

Alternate Names for HIF-1 alpha

 
  • ARNT-interacting protein
  • HIF1-alpha
  • HIF-1-alpha
  • BHLHE78
  • Member of PAS protein 1
  • member of PAS superfamily 1
  • Class E basic helix-loop-helix protein 78
  • hypoxia-inducible factor 1-alpha
  • Basic-helix-loop-helix-PAS protein MOP1
  • PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
  • MOP1HIF1-ALPHA
  • PAS domain-containing protein 8
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor)

Related Products by Gene

Background

 
HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.