HIF-1 alpha Antibody (NB100-105)
HIF-1 alpha Antibody (H1alpha67)
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anti-Hypoxia inducible factor 1 alpha subunit antibody, anti-Hypoxia inducible factor 1 alpha isoform I.3 antibody, anti-Hypoxia-inducible factor 1 alpha antibody, Anti-Basic helix loop helix PAS protein MOP1 antibody, Anti-PASD8 antibody, anti-PASD 8 antibody, Anti-MOP1 antibody, Anti-MOP 1 antibody, Anti-Member of the PAS Superfamily 1 antibody, Anti-Member of PAS superfamily 1 antibody, Anti-Member of PAS protein 1 antibody, Anti-Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody, anti-Hypoxia inducible factor 1 alpha subunit basic helix antibody, Anti-Hypoxia inducible factor 1 alpha antibody, anti-HIF-1alpha antibody, Anti-HIF1A antibody, anti-HIF1 Alpha antibody, Anti-HIF 1A antibody, anti-bHLHe78 antibody, Anti-ARNT interacting protein antibody, |
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HIF-1 alpha Antibody (H1alpha67) Summary: | Species: | Bv, Ft, Hu, Mk, Mu, Po, Rb, Rt, Sh |
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| Applications: | ChIP, ICC, IF, IHC, IHC-Fr, IHC-P, IP, WB |
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| Clonality: | Monoclonal |
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| | Gene: | HIF1A |
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| Purity: | Protein G purified |
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| Host: | Mouse |
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| | Specificity: | This is specific for HIF-1 alpha. |
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| Publications: | Antibody has been mentioned in at least 192 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional HIF-1 alpha Products HIF-1 alpha Antibody (H1alpha67) Details: | Clone: | H1alpha67 |
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| Isotype: | IgG2b |
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| Immunogen: | Fusion protein containing amino acids 432-528 of human HIF-1alpha. |
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| Localization: | HIF-1 is a nuclear protein. |
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NB 100-105 recognizes human, monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret HIF-1alpha. |
| Uses: | By Western blot, this antibody recognizes bands at 120 kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1alpha. This antibody has been used to immunoprecipitate human HIF-1 alpha. For ChIP, see Kong, et al. For WB, testing on nuclear extracts is recommended. |
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| Dilutions: | Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen 1:25 - 1:100, Immunohistochemistry-Paraffin 1:25 - 1:100, immunoprecipitation, Western Blot 1:500 |
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| TMW: | 97 kDa |
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| Unit Size: | 0.25 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 4.0 mg/ml |
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| Positive Controls: | 3 Positive Controls |
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| Conjugated Antibodies: | 5 Conjugated Antibodies |
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Notes: There are reports that this antibody does not detect mouse in IHC-paraffin embedded tissue. It does work to detect mouse protein in Western blot. Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | PBS |
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| Preservative: | 0.05% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
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Images (2) Reviews (8) Related Products (109) Exploring HIF-1 alpha Working with HIF-1 alpha
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with HIF-1 alpha
HIF-1 alpha Antibody (H1alpha67) Images (2)-Immunohistochemistry-NB100-105-img0001.jpg "IHC: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - IHC staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.") IHC: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - IHC staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining. |  Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Western Blot analysis of 50ug cobalt chloride (CoCl2) induced COS-7 nuclear extracts (NB800-PC26). |
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Reviewed by:
Huang Li-Ju 12/14/2011
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Application:
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Western Blot
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Sample Tested:
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Rat liver
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Species:
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Rat
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Sample Pretreated:
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Sepsis induction
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Blocking Buffer:
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5% nonfat milk in TBST
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Dilution Ratio:
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1:500
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Incubation Time:
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Over night
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Incubation Temperature:
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4 Celsius
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Description:
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anti-mouse
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Dilution Ratio:
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1:10000
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Reviewed by:
Hung-Kong Chen 12/9/2011
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Application:
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Western Blot
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Sample Tested:
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Mouse auditory cultured cell
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Sample Loaded:
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30ug
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Species:
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Mouse
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Sample Pretreated:
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Cell lysis in RIPA
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Results:
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HIF-1 alpha degradation was quick, and our result was still detected by your antibody.
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Dilution Ratio:
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1:1000
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Incubation Time:
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1 hour
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Description:
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anti-mouse HRP
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Reviewed by:
Shu-Wen Hsuan 11/21/2011
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Application:
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Western Blot
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Sample Tested:
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Human
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Species:
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Human
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Blocking Buffer:
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5% non-fat milk in TBST
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Dilution Ratio:
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1:500
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Incubation Time:
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overnight
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Method:
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Wesern Lighting plus ECL
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Reviewed by:
Qing Yang 9/30/2011
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Application:
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Western Blot
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Sample Tested:
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MCF-7
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Sample Loaded:
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100ug
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Species:
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Human
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Sample Pretreated:
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normoxia or hypoxia
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Results:
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HIF-1 alpha has a half-life of approximately 1-5 min in normoxic conditions, so sometimes the failure to detect its protein is due to the postpone during the sample collection. I strongly suggest all the procedures completed entirely on ice and quickest lysis, processing and storage. Even though, this good antibody is still highly recommended. Cells from MCF-7 grown under normoxia or hypoxia were lysed for HIF-1 alpha blotting. For the best signal, we suggest higher amount of protein (80-120ug) and traditional tank (wet) electroblotting protocol. 10v overnight transferring is the longest but most efficient way to transfer big proteins as Hif-1a. 1:500 dilution of this Ab and 1:2000 secondary Ab work well.
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Pub Med ID:
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21193031
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Blocking Buffer:
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5% Milk in TBST
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Concentration:
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5%
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Blocking Time:
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2 hours
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Blocking Temperature:
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20 Celsius
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Dilution Ratio:
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1:500
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Incubation Time:
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16 hours
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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5% milk TBST
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Description:
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HRP labeled anti-mouse ab
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Manufacturer Catalog #:
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7076 from cell signaling
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Dilution Ratio:
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1:2000
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Method:
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HRP
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Exposure Time:
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1 MIN
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Did you observe bands?:
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Yes
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Specific Bands:
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90 kDa
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Reviewed by:
Gina Capley 9/1/2010
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Application:
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Western Blot
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Sample Tested:
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endothelial cell nuclear extract
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Sample Loaded:
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40ug
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Species:
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Rat
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Sample Pretreated:
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hypoxia
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Results:
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Good results using this Ab for Western blots and ChIP analysis.
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Blocking Buffer:
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PBS + 5% NFDM + 0.05% Tween 20
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Blocking Time:
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1 hour
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Blocking Temperature:
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RT
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Dilution Ratio:
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1:250
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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PBS + 5% NFDM + 0.05% Tween 20
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Description:
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GaM=HRP
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Dilution Ratio:
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1:7500
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Method:
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chemiluminescence
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Exposure Time:
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15 min
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Positive Control:
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nuclear extract from same cells
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Did you observe bands?:
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Yes
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Specific Bands:
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60, 98 kDa
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Reviewed by:
Anonymous 5/17/2010
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Application:
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Western Blot
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Sample Tested:
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mouse cortical neurons
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Sample Loaded:
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40ug
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Species:
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Mouse
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Sample Pretreated:
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DFO
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Lot:
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AA1
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Results:
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I tested this Hif1a antibody on mouse primary cortical neuronal culture treated with 100uM DFO for over 4 hours. I collected the total lysate using RIPA buffer with 0.1% SDS, sonicated the lysates, and heated up to 90ºC for 5 minutes in SDS-containing sample buffer before loading on an SDS-PAGE gel. I used 1:1000 dilution for the Hif1a antibody (overnight), and 1:20000 anti-mouse secondary antibody for 1 hour. With a few background bands, this antibody works well to detect the upregulation of Hif1a under hypoxic conditions.
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Blocking Buffer:
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5% milk in TBST
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Blocking Time:
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2hrs
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Blocking Temperature:
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room temp
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Dilution Ratio:
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1:1000
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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blocking buffer
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Description:
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anti-mouse IgG-HRP
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Manufacturer Catalog #:
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Promega W402B
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Dilution Ratio:
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1:20000
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Method:
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ECL
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Exposure Time:
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2 minutes
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Positive Control:
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none
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Negative Control:
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untreated neurons
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Did you observe bands?:
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Yes
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Specific Bands:
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120 kDa
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Non-specific Bands:
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67 kDa
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Reviewed by:
Anonymous 2/10/2010
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Application:
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ICC
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Sample Tested:
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HUVEC & Candida albicans
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Species:
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Other
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Species Other:
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Yeast and Human
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Lot:
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Z5
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Results:
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Works fine with HUVEC but not when Candida albicans is present. The Candida hyphae bind the Ab w/ much greater affinity than the EC and act as a sink leaving the EC completely unstained.
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Blocking Buffer:
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PBST +1% BSA
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Blocking Time:
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30 min
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Blocking Temperature:
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25 Celsius
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Dilution Ratio:
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1/100
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Incubation Time:
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18 hrs
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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PBST + 1% BSA
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Description:
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anti-mouse IgG
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Manufacturer Catalog #:
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Invitrogen A11004
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Dilution Ratio:
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1/1000
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Reviewed by:
Anonymous 11/15/2011
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Application:
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IF
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Sample Tested:
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Human tissues
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Species:
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Human
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Sample Pretreatment:
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FFPE
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Results:
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It's specific, but not very intense under current expected conditions.
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Dilution Ratio:
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1:2000
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Incubation Time:
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overnight
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Description:
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Goat anti-mouse IgG [Alexa Flour 594]
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Visualization Method:
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Fluorescence microscopy
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Publications (192)HIF-1 alpha Antibody (H1alpha67) Product Specific:- Bohonowych JE, Peng S, Gopal U et al. Comparative analysis of novel and conventional Hsp90 inhibitors on HIF activity and angiogenic potential in clear cell renal cell carcinoma: implications for clinical evaluation. BMC Cancer. 2011 Dec 15;11(1):520 . [PMID: 22172030]
- Schrobback K, Malda J, Crawford R, et al. Effects of Oxygen on Zonal Marker Expression in Human Articular Chondrocytes. Tissue Eng Part A. 2011 Nov 19. [PMID 22097912]
more... - Pascal LE, Ai J, Rigatti LH, et al. EAF2 loss enhances angiogenic effects of Von Hippel-Lindau heterozygosity on the murine liver and prostate.Angiogenesis. 2011 Sep;14(3):331-43.[PMID 21638067]
- Kummar S, Raffeld M, Juwara L, et al. Multihistology, Target-Driven Pilot Trial of Oral Topotecan as an Inhibitor of Hypoxia-Inducible Factor-1{alpha} (HIF-1{alpha}) in Advanced Solid Tumors.Clin Cancer Res. 2011 Aug 1;17(15):5123-31.[PMID 21673063]
- Morine Y, Shimada M, Iwahashi S,et al.Role of histone deacetylase expression in intrahepatic cholangiocarcinoma. Surgery. 2011 Oct 8.[PMID: 21982637]
- Kaie K, Hideaki I, Chisato K, et al. Does Hypoxic Preconditioning Induce Angiogenesis and Protect against Focal Cerebral Ischemic Damage in Rats? The Kitakanto medical journal. 2011;61(1):1-8.
- Chaudhuri S, McCullough SS, Hennings L, et al. Acetaminophen hepatotoxicity and HIF-1alpha induction in mice occurs without hypoxia. Toxicol Appl Pharmacol. 2011 Feb 10.[PMID: 21316383]
- Kwon T-G, Zhao X, Yang Q, et al. Physical and functional interactions between Runx2 and HIF-1[alpha] induce vascular endothelial growth factor gene expression. J Cell Biochem. 2011 Jul 25.[PMID 21793044]
- Leh S, Hultström M, Rosenberger C, Iversen BM. Afferent arteriolopathy and glomerular collapse but not segmental sclerosis induce tubular atrophy in old spontaneously hypertensive rats. Virchows Arch. 2011 Jun 10.[PMID 21660521]
- Jian J, Yang Q, Dai J, et al. Effects of iron deficiency and iron overload on angiogenesis and oxidative stress-a potential dual role for iron in breast cancer. Free Radic Biol Med. 2011 Apr 1;50(7):841-7. [PMID: 21193031]
- Chen H, Kluz T, Zhang R, Costa M. Hypoxia and Nickel inhibit histone demethylase JMJD1A and repress Spry2 expression in human bronchial epithelial BEAS-2B cells. Carcinogenesis. 2010 Sep 29. [PMID: 20881000]
- Wen W, Ding J, Sun W et al. Suppression of cyclin D1 by hypoxia-inducible factor-1 via direct mechanism inhibits the proliferation and 5-fluorouracil-induced apoptosis of A549 cells. Cancer Res. 2010 Mar 1;70(5):2010-9.[PMID: 20179204]
- Evens AM, Sehn LH, Farinha P et al. Hypoxia-inducible factor-1 {alpha} expression predicts superior survival in patients with diffuse large B-cell lymphoma treated with R-CHOP. J Clin Oncol. 2010 Feb 20;28(6):1017-24. [PMID: 20048181]
- Regazzetti C, Bost F, Le Marchand-Brustel Y, et al. Insulin induces REDD1 expression through hypoxia-inducible factor 1 activation in adipocytes. J Biol Chem. 2010 Feb 19;285(8):5157-64. [PMID: 1999631] (Mouse, WB)
- Neusser MA, Lindenmeyer MT, Moll AG, et al. Human nephrosclerosis triggers a hypoxia-related glomerulopathy. Am J Pathol. 2010 Feb;176(2):594-607. [PMID: 20019191]
- Moser C, et al. ENMD-1198, a novel tubulin-binding agent reduces HIF-1alpha and STAT3 activity in human hepatocellular carcinoma(HCC) cells, and inhibits growth and vascularization in vivo.BMC Cancer. 2008 Jul 23;8:206.
- Hsieh CH, Kuo JW, Lee YJ, et al. Construction of mutant TKGFP for real-time imaging of temporal dynamics of HIF-1 signal transduction activity mediated by hypoxia and reoxygenation in tumors in living mice. J Nucl Med. 2009 Dec;50(12):2049-57. [PMID: 19910419]
- Rey S, Lee K, Wang CJ, et al. Synergistic effect of HIF-1alpha gene therapy and HIF-1-activated bone marrow-derived angiogenic cells in a mouse model of limb ischemia. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20399-404. [PMID: 19948968]
- Tanaka T, Wakamatsu T, Daijo H, et al. Persisting mild hypothermia suppresses hypoxia-inducible factor 1{alpha} protein synthesis and HIF-1-mediated gene expression. Am J Physiol Regul Integr Comp Physiol. 2009 Dec 30. [PMID: 20042684]
- Mahimainathan L, Ghosh-Choudhury N, Venkatesan B, et al. TSC2 Deficiency Increases PTEN via HIF1{alpha}. J Biol Chem 2009;284(41):27790-27798. [PMID: 19648120]
- Doroudgar S, Thuerauf DJ, Marcinko MC, et al. Ischemia Activates the ATF6 Branch of the Endoplasmic Reticulum Stress Response. J Biol Chem 2009;284(43):29735-29745. [PMID: 19622751]
- Lee K, Zhang H, Qian DZ, et al. Acriflavine inhibits HIF-1 dimerization, tumor growth, and vascularization. PNAS 2009;106(42):17910-17915.
- Rezvani HR, Mahfouf W, Ali N, Chemin C, et al. Hypoxia-inducible factor-1{alpha} regulates the expression of nucleotide excision repair proteins in keratinocytes. Nucleic Acids Res. 2009 Nov 24. [PMID: 19934262]
- Nomura M, Ishii H, Kawakami A, et al. Inhibition of Hepatic Neiman-Pick C1-Like 1 Improves Hepatic Insulin Resistance.Am J Physiol Endocrinol Metab. 2009 Aug 4. [PMID: 19654286]
- Hammerschmidt E, Loeffler I, Wolf G. Morg1 heterozygous mice are protected from acute renal ischemia-reperfusion injury. Am J Physiol Renal Physiol. 2009 Nov;297(5):F1273-87. [PMID: 19726548]
- Sims JR, Lee SW, Topalkara K, et al. Sonic hedgehog regulates ischemia/hypoxia-induced neural progenitor proliferation. Stroke. 2009 Nov;40(11):3618-26. [PMID: 19762700]
- Luo, W., Zhong, J., Chang, R., Hu, H., Pandey, A., Semenza, G.L. HSP70 and CHIP selectively mediate Ubiquitination and degradation of hypoxia-inducible factor (HIF)-1a but not HIF-2a J. Biol. Chem. jbc.M109.068577First Published on November 23, 2009, doi:10.1074/jbc.M109.068577
- Palladino MA, Powell JD, Korah N, et al. Expresion Localization of Hypoxia-Inducible Factor-1 Subunits in the Adult Rat Epididymis. Biol Reprod. 2004 Apr;70(4):1121-30. [pubmed: 1466820]
- Zhang H, Bosch-Marce M, Shimoda LA, et al. Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia. J Biol Chem. 2008 Apr 18;283(16):10892-903. [PMID: 18281291]
- Tsuruda T, Hatakeyama K, Masuyama H, et al. Pharmacological Stimulation of Soluble Guanylate Cyclase Modulates Hypoxia Inducible Factor-1{alpha} in Rat Heart. Am J Physiol Heart Circ Physiol 2009:00503.02009. [pubmed: 19684186]
- Thangarajah H, Yao D, Chang EI, et al. The molecular basis for impaired hypoxia-induced VEGF expression in diabetic tissues. PNAS 2009;106(32):13505-13510. [pubmed: 19666581]
- Hu Y, Kirito K, Yoshida K, et al. Inhibition of hypoxia-inducible factor-1 function enhances the sensitivity of multiple myeloma cells to melphalan. Mol Cancer Ther 2009;8(8):2329-2338. [pubmed: 19671732]
- Halberg N, Khan T, Trujillo ME, et al. Hypoxia-Inducible Factor 1{alpha} Induces Fibrosis Insulin Resistance in White Adipose Tissue. Mol Cell Biol 2009;29(16):4467-4483. [pubmed: 19546236]
- Chen A, Cuevas I, Kenny PA, et al. Endothelial Cell Migration Vascular Endothelial Growth Factor Expression Are the Result of Loss of Breast Tissue Polarity. Cancer Res 2009;69(16):6721-6729. [pubmed: 19654314]
- Bhaskar PT, Nogueira V, Patra KC, et al. mTORC1 Hyperactivity Inhibits Serum Deprivation-Induced Apoptosis via Increased Hexokinase II GLUT1 Expression, Sustained Mcl-1 Expression, Glycogen Synthase Kinase 3{beta} Inhibition. Mol Cell Biol 2009;29(18):5136-5147. [pubmed: 19620286]
- Benita Y, Kikuchi H, Smith AD, et al. An integrative genomics approach identifies Hypoxia Inducible Factor-1 (HIF-1)-target genes that form the core response to hypoxia. Nucleic Acids Res 2009;37(14):4587-4602. [pubmed: 19491311]
- Hsu K, Chi N, Gucek M, et al. Miltenberger blood group antigen type III (Mi.III) enhances the expression of b 3. Blood 2009;114(9):1919-1928. [pubmed: 19564639]
- Torii S, Kobayashi K, Takahashi M, et al. Magnesium Deficiency Causes Loss of Response to Intermittent Hypoxia in Paraganglion Cells. J Biol Chem 2009;284(28):19077-19089. [pubmed: 19433582]
- Shackelford DB, Vasquez DS, Corbeil J, et al. mTOR HIF-1{alpha}-mediated tumor metabolism in an LKB1 mouse model of Peutz-Jeghers syndrome. PNAS 2009;106(27):11137-11142. [pubmed: 19541609]
- Rapisarda A, Hollingshead M, Uranchimeg B, et al. Increased antitumor activity of bevacizumab in combination with hypoxia inducible factor-1 inhibition. Mol Cancer Ther 2009;8(7):1867-1877. [pubmed: 19584228]
- Kawanami D, Mahabeleshwar GH, Lin Z, et al. Kruppel-like Factor 2 Inhibits Hypoxia-inducible Factor 1{alpha} Expression Function in the Endothelium. J Biol Chem 2009;284(31):20522-20530. [pubmed: 19491109]
- Fang H-Y, Hughes R, Murdoch C, et al. Hypoxia-inducible factors 1 2 are important transcriptional effectors in primary macrophages experiencing hypoxia. Blood 2009;114(4):844-859. [pubmed: 19454749]
- Bhaskar PT, Nogueira V, Patra KC, et al. mTORC1 Hyperactivity Inhibits Serum Deprivation Apoptosis via Increased HKII GLUT1 Expression, Sustained Mcl-1 Expression, GSK3{beta} Inhibition. Mol Cell Biol 2009:MCB.01946-01908. [pubmed: 19620286]
- Aubert S, Fauquette V, Hemon B, et al. MUC1, a New Hypoxia Inducible Factor Target Gene, Is an Actor in Clear Renal Cell Carcinoma Tumor Progression. Cancer Res 2009;69(14):5707-5715. [pubmed: 19549898]
- Loinard C, Ginouves A, Vilar J, et al. Inhibition of Prolyl Hydroxylase Domain Proteins Promotes Therapeutic Revascularization. Circulation 2009;120(1):50-59. [pubmed: 19546390]
- Rhoads RP, Johnson RM, Rathbone CR, et al. Satellite cell-mediated angiogenesis in vitro coincides with a functional hypoxia-inducible factor pathway. Am J Physiol Cell Physiol 2009;296(6):C1321-1328. [pubmed: 19386789]
- Aubert S, Fauquette V, Hemon B, et al. MUC1, a New Hypoxia Inducible Factor Target Gene, Is an Actor in Clear Renal Cell Carcinoma Tumor Progression. Cancer Res 2009:0008-5472.CAN-0008-4905. [pubmed: 19549898]
- Benita Y, Kikuchi H, Smith AD, et al. An integrative genomics approach identifies Hypoxia Inducible Factor-1 (HIF-1)-target genes that form the core response to hypoxia. Nucleic Acids Res 2009:gkp425. [pubmed: 19491311]
- Halberg N, Khan T, Trujillo ME, et al. HIF 1 alpha Induces Fibrosis Insulin Resistance in White Adipose Tissue. Mol Cell Biol 2009:MCB.00192-00109. [pubmed: 19546236]
- Kimbrel EA, Kung AL. The F-box Protein {beta}-TrCp1/Fbw1a Interacts with p300 to Enhance {beta}-Catenin Transcriptional Activity. J Biol Chem 2009;284(19):13033-13044. [pubmed: 19297328 ]
- Guma M, Rius J, Duong-Polk KX, et al. Genetic pharmacological inhibition of JNK ameliorates hypoxia-induced retinopathy through interference with VEGF expression. PNAS 2009;106(21):8760-8765. [pubmed: 19433784]
- Ghosh AK, Shanafelt TD, Cimmino A, et al. Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells. Blood 2009;113(22):5568-5574. [pubmed: 19336759]
- Lomb DJ, Desouza LA, Franklin JL, et al. Prolyl Hydroxylase Inhibitors Depend on Extracellular Glucose Hypoxia-Inducible Factor (HIF)-2{alpha} to Inhibit Cell Death Caused by Nerve Growth Factor (NGF) Deprivation: Evidence that HIF-2{alpha} Has a Role in NGF-Promoted Survival of Sympathetic Neurons. Mol Pharmacol 2009;75(5):1198-1209. [pubmed: 19204094]
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Jump to: Conjugated Antibodies, Positive Controls, Antibody Packs, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| HIF-1 alpha Antibody (H1alpha67) [DyLight 488] | NB100-105G | Bv, Ft, Hu, Mk, Mu, Po, Rb, Rt, Sh | ChIP, IF, IHC, IHC-Fr, IP, WB | | (154) | | | | HIF-1 alpha Antibody (H1alpha67) [DyLight 650] | NB100-105C | Bv, Ft, Hu, Mk, Mu, Po, Rb, Rt, Sh | ChIP, IF, IHC, IHC-Fr, IP, WB | | (154) | | | | HIF-1 alpha Antibody (H1alpha67) [HRP] | NB100-105H | Bv, Ft, Hu, Mk, Mu, Po, Rb, Rt, Sh | ChIP, IF, IHC, IHC-Fr, IP, WB | (2) | (150) | | | | HIF-1 alpha Antibody (H1alpha67) [Biotin] | NB100-105B | Bv, Ft, Hu, Mk, Mu, Po, Rb, Rt, Sh | ChIP, IF, IHC, IHC-Fr, IP, WB | (2) | (150) | | | | HIF-1 alpha Antibody (H1alpha67) [DyLight 550] | NB100-105R | Bv, Ft, Hu, Mk, Mu, Po, Rb, Rt, Sh | ChIP, IF, IHC, IHC-Fr, IP, WB | | (150) | | |
Positive ControlsAntibody Packs (3)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| HIF-1 alpha Antibody Pack 5 | NB100-900WB | Hu, Mk, Mu, Rt | ChIP, FACS, ICC, IF, IHC, IHC-Fr, IHC-P, IP, WB | (11) | (36) | | | | HIF-1 alpha Antibody Pack 5Vials | NB100-905 | Hu, Mu | ChIP, FACS, ICC, IHC, IHC-Fr, IHC-P, IP, WB | (16) | (4) | | | | HIF-1 alpha Antibody Pack 4 vials | NB100-901IHC | Hu, Mu, Rt | FACS, ICC, IF, IHC, IHC-Fr, IHC-P, IP, WB | (15) | (5) | | |
Antibody Pairs (1)Lysates (1)Peptides and ProteinsPrimary Antibodies (92)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| HIF-1 alpha Antibody | NB100-479 | Hu, Mk, Mu, Rt | ICC, IF, IHC-Fr, IHC-P, WB | (4) | (35) | | | | HIF-1 alpha Antibody (H1alpha67) | NB100-123 | Bv, Ft, Hu, Mk, Mu, Po, Rt, Sh | IF, IHC, IHC-P, IP, WB | (4) | (31) | | | | HIF-1 alpha Antibody | NB100-134 | Hu, Mk, Mu, Rt | ChIP, IHC-P, IP, WB | (3) | (31) | | | | HIF-1 alpha Antibody (ESEE122) | NB100-131 | Bv, Ca, Hu, Mu, Rt | ICC, IF, IHC-Fr, IHC-P, IP, WB | (6) | (23) | | | | HIF-1 alpha Antibody | NB100-449 | Fi, Hu, Mk, Mu, Rt | FACS, IHC-P, IP, WB | (4) | (29) | | | | ... and 87 more. See All » |
RNAi (2)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Mouse IgG Antibody [Biotin] | NB720-B | Mu | ELISA, IHC-P, WB | | | | | | Mouse IgG Antibody [HRP] | NB720-H | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [Alkaline Phosphatase] | NB720-AP | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [FITC] | NB720-F | Mu | ICC, IHC-P | | (2) | | | | Mouse IgG Antibody | NB120-7056 | Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-) | ELISA, IHC-P, IP, WB | | | | | | See All » |
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