HIF-1 alpha Antibody (H1alpha67) 0.1 ml

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Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Expression of HIF-1 in intresititial pig cells under 12% of 02 condition. Image from verified customer review.
Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis of 50ug cobalt chloride (CoCl2) induced COS-7 nuclear extracts (NB800-PC26).
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Ordering Information: NB100-105

  • Catalog Number
    NB100-105
  • Displayed Format
    Unconjugated
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HIF-1 alpha Antibody (H1alpha67) Summary

Species Human, Mouse, Rat, Bovine, Ferret, Porcine, Primate, Rabbit, Sheep, Xenopus, Yeast
Tested Applications WB, ChIP, ELISA, FLOW, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Monoclonal
Host
Mouse
Gene
HIF1A
Purity
Protein G purified
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HIF-1 alpha Antibody (H1alpha67) Details

Clone
H1alpha67
Isotype
IgG2b
Immunogen
Fusion protein containing amino acids 432-528 of human HIF-1 alpha. [UniProt# Q16665]
Localization
HIF-1 is a nuclear protein.

Species Reactivity

Human, monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret. Reactivity with Candida albicans reported by a customer review. Reactivity with Xenopus reported in the scientific literature (PMID: 18303027).
Reviewed Species Read 21 rated 4.3
using NB100-105 in the following species:

Publications Read Publications using NB100-105 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA
  • Flow Cytometry
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:20-1:50
  • Immunohistochemistry-Frozen 1:20-1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Immunoprecipitation 1:10-1:500
Application Notes
This HIF-1 alpha (H1alpha67) antibody is useful for Chromatin Immunoprecipitation (PMID: 21871655), ELISA (PMID: 20042684), Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band can be seen at 120 kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended. This antibody has been used to immunoprecipitate human HIF-1 alpha. For ChIP, refer to research papers with PubMed ID 16204079 and 21871655. This product has been cited for Gel Super Shift Assays in PubMed ID 22411794.
Molecular Weight
120 kDa
Positive Controls
Other Available
Formats
Alexa Fluor (R) 405 Labeled NB100-105AF405
Alexa Fluor (R) 488 Labeled NB100-105AF488
Alexa Fluor (R) 647 Labeled NB100-105AF647
Alexa Fluor (R) 700 Labeled NB100-105AF700
Biotin Labeled NB100-105B
DyLight 405 Labeled NB100-105V
DyLight 488 Labeled NB100-105G
DyLight 550 Labeled NB100-105R
DyLight 650 Labeled NB100-105C
FITC Labeled NB100-105F
HRP Labeled NB100-105H
Reviewed Applications
Read 21 Review rated 4.3
using
NB100-105 in the following applications:

Publications
Read Publications using
NB100-105 in the following applications:

Contact Information

Product PDFs

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Reviews

Average Rating: 4.3
Recent Reviews
WB Tested In Human
Reviewed On: 07/18/2014
Rated: 4

HIF-1 alpha NB100-105
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Publications

HIF-1 alpha Antibody (NB100-105) has been mentioned in at least 447 publications.
Earn rewards if you have published using HIF-1 alpha Antibody (NB100-105).

Notes

There are reports that this antibody does not detect mouse in IHC-paraffin embedded tissue. It does work to detect mouse protein in Western blot.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Unit Size
0.1 ml
Concentration
1.2 mg/ml
Preservative
0.02% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol HIF1A
Entrez
OMIM
Uniprot

Alternate Names for HIF-1 alpha Antibody (H1alpha67)

  • ARNT-interacting protein
  • Basic-helix-loop-helix-PAS protein MOP1
  • BHLHE78
  • Class E basic helix-loop-helix protein 78
  • HIF-1-alpha
  • HIF1-alpha
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor)
  • hypoxia-inducible factor 1-alpha
  • Member of PAS protein 1
  • member of PAS superfamily 1
  • MOP1HIF1-ALPHA
  • PAS domain-containing protein 8
  • PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
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Related Products by Gene

Background

HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.

Reviews for HIF-1 alpha Antibody (NB100-105) (21) 4.321

Read what people are saying who have used HIF-1 alpha Antibody (NB100-105).

We have 21 reviews tested in 4 species: Human, Mouse, Rat, Other.
We have 21 reviews tested in 3: Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.
Average Rating: 4.3
(Based on 21 reviews)

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Sort 21 reviews for HIF-1 alpha Antibody (NB100-105) by:
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Excellent
(7)
Very Good
(13)
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(1)
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WB
(16)
IHC
(1)
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(4)
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Human
(9)
Mouse
(6)
Rat
(3)
Other
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Images Ratings Applications Species Reviewed By Date Details
Western Blot HIF-1 alpha NB100-105
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Very Good
WB Human Anonymous 07/18/2014
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Summary

ApplicationWestern Blot
Sample TestedCaki-1 cell lysate
Sample PretreatmentCoCl2 100uM, 300 uM
SpeciesHuman

Blocking

Blocking Details1xPBST with 5% non-fat milk, RT 1 hour

Primary Anitbody

Dilution Ratio1xpBST with 5% non-fat milk, overnight 4C, 1:1000 dilution

Secondary Antibody

Secondary Manufacturer Cat#sc-2005
Secondary Concentration1:2000 dilution

Details

Detection NotesECL, exposured for 5 min
  Excellent
WB Human Aparna Akolkar 07/01/2014
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Summary

ApplicationWestern Blot
Sample TestedHela Cell Lysate
SpeciesHuman
Western Blot HIF-1 alpha NB100-105
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Excellent
WB Human Anonymous 05/09/2014
Show

Summary

ApplicationWestern Blot
Sample TestedHeLa whole cell lysate
Sample Pretreatment50 micrograms of protein lysate.
SpeciesHuman

Blocking

Blocking DetailsTBS milk 5% for 1 hour room temperature

Primary Anitbody

Dilution RatioAntibody diluition 1:1000 in TBS milk 5%, incubation time 2 hours room temperature

Secondary Antibody

Secondary Manufacturer Cat#santa cruz

Details

Detection NotesECL plus Western Blotting Detection Kit 5 minutes incubation room temperature, Phosphorimager detection system,
Western Blot HIF-1 alpha NB100-105
Click to Enlarge
Excellent
WB Mouse Cidi Chen 04/28/2014
Show

Summary

ApplicationWestern Blot
Sample Testedmouse brain lysate
Sample Pretreatment25 ug
SpeciesMouse

Blocking

Blocking Details7% Milk 30 min RT

Primary Anitbody

Dilution Ratio1: 1000, 4 degree overnight in TBST

Secondary Antibody

Secondary Manufacturer Cat#NBP1-47596
Secondary Concentration1ug/mL
Western Blot HIF-1 alpha NB100-105
Click to Enlarge
Very Good
WB Mouse Anonymous 04/28/2014
Show

Summary

ApplicationWestern Blot
Sample Testedmouse
SpeciesMouse
Immunohistochemistry HIF-1 alpha NB100-105
Click to Enlarge
Very Good
IHC Other Anonymous 04/14/2014
Show

Summary

ApplicationImmunohistochemistry
SpeciesOther
Other Speciesporicine
FileView PDF
Immunocytochemistry/Immunofluorescence HIF-1 alpha NB100-105
Click to Enlarge
Very Good
ICC/IF Other Anonymous 04/14/2014
Show

Summary

ApplicationImmunocytochemistry/Immunofluorescence
SpeciesOther
Other Speciesporicine
FileView PDF
Western Blot HIF-1 alpha NB100-105
Click to Enlarge
Very Good
WB Mouse Anonymous 03/28/2014
Show

Summary

ApplicationWestern Blot
SpeciesMouse
Special ApplicationsPrimary 1:1000Secondary 1: 10000
FileView PDF
Western Blot HIF-1 alpha NB100-105
Click to Enlarge
Excellent
WB Human Anonymous 08/23/2012
Show

Summary

ApplicationWestern Blot
Sample TestedWHOLE CELL LYSATE
Sample Pretreatment100ug
SpeciesHuman
LotAB7

Blocking

Blocking Details5% milk in TBS-T buffer, room temperature for one hours

Primary Anitbody

Dilution Ratio1:500 in 5% milk -TBS-T solution, 4C for overnight

Secondary Antibody

Secondary Manufacturer Cat#Santa Cruz, Cat# sc-2005
Secondary Concentration1:4000

Details

Detection NotesECL solution for 5min, then exposure to film 3-5min
Immunocytochemistry/Immunofluorescence HIF-1 alpha NB100-105
Click to Enlarge
Excellent
ICC/IF Rat KAMESWARAN RAVICHANDRAN 08/22/2012
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedRAT KIDNEY
Sample PretreatmentRAT KIDNEY WAS FIXED IN OCT.5um sections were cut.
SpeciesRat
LotF2
CommentsPMID 21270095

Blocking

Blocking Detailsserum blocking for 30 minutes.

Primary Anitbody

Dilution Ratio1:100, overnight incubation,room temperature.

Details

Detection Notes1:1000 Alexa Flour 488, 3:1000 Hoechst 33342.
Fixation Details70% acetone 30% methanol.
Wash Description1xPBS, 5 minutes each,3 times.
Western Blot HIF-1 alpha NB100-105
Click to Enlarge
Excellent
WB Human Anonymous 08/22/2012
Show

Summary

ApplicationWestern Blot
Sample Testedhuman adrenal cancer cells (whole cell lysate)
Sample Pretreatmentcells were cultured under normoxic conditions. 40ug of protein extracts were used for analysis.
SpeciesHuman

Blocking

Blocking Details5% non fat dry milk in PBS 1x + 0,1% tween; 1 h at RT

Primary Anitbody

Dilution Ratio1:500; 5% non fat dry milk in PBS 1x + 0,1% tween; o.n. at +4°C

Secondary Antibody

Secondary Manufacturer Cat#Sigma-Aldrich
Secondary Concentration1:4000; 1 h at RT

Details

Detection Notesenhanced chemiluminescence (ECL) assay; exposure: 30 min
Western Blot HIF-1 alpha NB100-105
Click to Enlarge
Excellent
WB Mouse Anonymous 05/15/2012
Show

Summary

ApplicationWestern Blot
Sample Testedcell lysate
Sample PretreatmentA23187
SpeciesMouse

Blocking

Blocking Details10% milk

Primary Anitbody

Dilution Ratio1:500

Secondary Antibody

Secondary Concentration1:2000

Details

Detection NotesECL
Western Blot HIF-1 alpha NB100-105
Click to Enlarge
Very Good
WB Human Anonymous 05/11/2012
Show

Summary

ApplicationWestern Blot
Sample TestedHuman prostate cancer cells
Sample PretreatmentCells grown under normoxic and hypoxic conditions were scraped in cold PBS, centrifuged and resuspended in cold lysis buffer
SpeciesHuman
LotAB1
CommentsA good result of Novus' HIF-1 alpha Antibody (NB100-105) was obtained when we used a cell lysate with a plate (10 cm) of LNCaP. The cell line and the exposure time of plate was 20 minutes. We realized an immunoreactive band of about 120 KDa.

Blocking

Blocking Details5% dry milk inTBS 1X/tween (0.1%) buffer

Primary Anitbody

Dilution Ratio1:500, 4 Celcius, O/N

Secondary Antibody

Secondary Manufacturer Cat#Pierce Biotechnology
Secondary Concentration1:2000

Details

Detection NotesImmunoreactive bands were visualized by enhanced chemiluminescence (ECL) assay
Western Blot HIF-1 alpha NB100-105
Click to Enlarge
Very Good
WB Rat Huang Li-Ju 12/14/2011
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Summary

ApplicationWestern Blot
Sample TestedRat liver
Sample PretreatmentSepsis induction
SpeciesRat

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in TBST

Primary Anitbody

Dilution RatioDilution Ratio: 1:500, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Details

Detection NotesDetection Method: ECL
Western Blot HIF-1 alpha NB100-105
Click to Enlarge
Very Good
WB Mouse Hung-Kong Chen 12/09/2011
Show

Summary

ApplicationWestern Blot
Sample TestedMouse auditory cultured cell, Sample Amount: 30ug
Sample PretreatmentCell lysis in RIPA
SpeciesMouse
CommentsHIF-1 alpha degradation was quick, and our result was still detected by your antibody.

Blocking

Blocking DetailsBlocking Buffer: 1% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 1 hour

Details

Detection NotesDetection Method: Millipore ECL
  Very Good
WB Human Shu-Wen Hsuan 11/21/2011
Show

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in TBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: overnight

Details

Detection NotesDetection Method: Wesern Lighting plus ECL
  Average
ICC/IF Human Anonymous 11/15/2011
Show

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHuman tissues
Sample PretreatmentFFPE
SpeciesHuman
CommentsIt's specific, but not very intense under current expected conditions.

Blocking

Blocking DetailsBlocking Buffer: BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: overnight

Details

Detection NotesDetection Visualization Method: Fluorescence microscopy
  Very Good
WB Human Qing Yang 09/30/2011
Show

Summary

ApplicationWestern Blot
Sample TestedMCF-7, Sample Amount: 100ug
Sample Pretreatmentnormoxia or hypoxia
SpeciesHuman
CommentsHIF-1 alpha has a half-life of approximately 1-5 min in normoxic conditions, so sometimes the failure to detect its protein is due to the postpone during the sample collection. I strongly suggest all the procedures completed entirely on ice and quickest lysis, processing and storage. Even though, this good antibody is still highly recommended. Cells from MCF-7 grown under normoxia or hypoxia were lysed for HIF-1 alpha blotting. For the best signal, we suggest higher amount of protein (80-120ug) and traditional tank (wet) electroblotting protocol. 10v overnight transferring is the longest but most efficient way to transfer big proteins as Hif-1a. 1:500 dilution of this Ab and 1:2000 secondary Ab work well.
Pub Med ID21193031

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 2 hours, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:500, Incubation Dilution Buffer: 5% milk TBST, Incubation Time: 16 hours, Incubation Temp: 4 C

Secondary Antibody

Secondary Manufacturer Cat#7076 from cell signaling

Details

Detection NotesDetection Method: HRP, Exposure Time: 1 minute, Specific Bands: 90 kDa
  Very Good
WB Rat Gina Capley 09/01/2010
Show

Summary

ApplicationWestern Blot
Sample Testedendothelial cell nuclear extract, Sample Amount: 40ug
Sample Pretreatmenthypoxia
SpeciesRat
CommentsGood results using this Ab for Western blots and ChIP analysis.

Blocking

Blocking DetailsBlocking Buffer: PBS + 5% NFDM + 0.05% Tween 20, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:250, Incubation Dilution Buffer: PBS + 5% NFDM + 0.05% Tween 20, Incubation Time/Temp: overnight at 4C

Details

Detection NotesMethod: Chemiluminescence, Exposure Time: 15 min, Positive Control: nuclear extract from same cells, Specific Bands: 60, 98 kDa
  Very Good
WB Mouse Anonymous 05/17/2010
Show

Summary

ApplicationWestern Blot
Sample Testedmouse cortical neurons, Sample Amount: 40ug
Sample PretreatmentDFO
SpeciesMouse
LotAA1
CommentsI tested this Hif1a antibody on mouse primary cortical neuronal culture treated with 100uM DFO for over 4 hours. I collected the total lysate using RIPA buffer with 0.1% SDS, sonicated the lysates, and heated up to 90ºC for 5 minutes in SDS-containing sample buffer before loading on an SDS-PAGE gel. I used 1:1000 dilution for the Hif1a antibody (overnight), and 1:20000 anti-mouse secondary antibody for 1 hour. With a few background bands, this antibody works well to detect the upregulation of Hif1a under hypoxic conditions.

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: blocking buffer, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary Manufacturer Cat#Promega W402B

Details

Detection NotesDetection Method: ECL, Exposure Time: 2 min, Neg.Control: untreated neurons, Specific Bands: 120 kDa, Non-Specific Bands: 67 kDa

Comments

CommentsI tested this Hif1a antibody on mouse primary cortical neuronal culture treated with 100uM DFO for over 4 hours. I collected the total lysate using RIPA buffer with 0.1% SDS, sonicated the lysates, and heated up to 90ºC for 5 minutes in SDS-containing sample buffer before loading on an SDS-PAGE gel. I used 1:1000 dilution for the Hif1a antibody (overnight), and 1:20000 anti-mouse secondary antibody for 1 hour. With a few background bands, this antibody works well to detect the upregulation of Hif1a under hypoxic conditions.
  Very Good
ICC/IF Other x x 02/10/2010
Show

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHUVEC & Candida albicans
SpeciesOther
Other SpeciesYeast and Human
LotZ5
CommentsWorks fine with HUVEC but not when Candida albicans is present. The Candida hyphae bind the Ab w/ much greater affinity than the EC and act as a sink leaving the EC completely unstained.

Blocking

Blocking DetailsBlocking Buffer: PBST +1% BSA, Blocking Time: 30 minutes, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: PBST + 1% BSA, Dilution Ratio: 1/100, Incubation Time: 18 hours, Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary Manufacturer Cat#Invitrogen A11004

Publications for HIF-1 alpha Antibody (NB100-105) (447)

Read what people are writing who have used HIF-1 alpha Antibody (NB100-105).

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Bovine, Canine, Porcine, Primate, Rabbit, Sheep, Xenopus.

We have publications tested in 10 applications: WB, ChIP, ELISA, FLOW, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP.

Have you published using HIF-1 alpha Antibody (NB100-105)? Submit your review and earn rewards points which can be used for merchandise & discounts.
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(4)
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(231)
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(4)
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Publication Applications Species
Ryu J, Lee C-W, Hong K-H et al. Activation of fractalkine/CX3CR1 by vascular endothelial cells induces angiogenesis through VEGF-A/KDR reverses hindlimb ischaemia. Cardiovasc Res. May 1, 200878(2):333-40. [PMID: 18006432] (WB, Human) WB Human
Bohonowych JE, Peng S, Gopal U et al. Comparative analysis of novel and conventional Hsp90 inhibitors on HIF activity and angiogenic potential in clear cell renal cell carcinoma: implications for clinical evaluation. BMC Cancer;11(1):520. 2011 Dec 15. [PMID: 22172030] (WB, Human) WB Human
Duyndam, MC et al. Induction of vascular endothelial growth factor expression and hypoxia-inducible factor 1alpha protein by the oxidative stressor arsenite. J Biol Chem;276(51):48066-76. 2001 Dec 21. [PMID: 11641398] (WB, Human) WB Human
Alberghini A, Recalcati S, Tacchini L et al. Loss of the von Hippel Lindau tumor suppressor disrupts iron homeostasis in renal carcinoma cells. J Biol Chem. 280(34):30120-8. 2005 Aug 26. [PMID: 15985433] (WB, Human) WB Human
Liu W, Guo M, Xu YB et al. Induction of tumor arrest and differentiation with prolonged survival by intermittent hypoxia in a mouse model of acute myeloid leukemia. 107(2):698-707. 2006 Jan 15. [PMID: 16166593] (WB, Mouse) WB Mouse
Trentin, D et al. Peptide-matrix-mediated gene transfer of an oxygen-insensitive hypoxia-inducible factor-1alpha variant for local induction of angiogenesis. Proc Natl Acad Sci U S A. 103(8):2506-11. 2006 Feb 21. [PMID: 16477043] (WB, Human) WB Human
Hsu, T et al. Endocytic function of von Hippel-Lindau tumor suppressor protein regulates surface localization of fibroblast growth factor receptor 1 and cell motility. J Biol Chem. 281(17):12069-80. 2006 Apr 28. [PMID: 16505488] (WB, Human) WB Human
Bracken CP, Fedele AO, Linke S et al. Cell-specific regulation of hypoxia-inducible factor (HIF)-1alpha HIF-2alpha stabilization transactivation in a graded oxygen environment. J Biol Chem;281(32):22575-85. 2006 Aug 11. [PMID: 16760477] (WB, Human) WB Human
McMahon S, Charbonneau M, Grandmont S et al. Transforming growth factor beta1 induces hypoxia-inducible factor-1 stabilization through selective inhibition of PHD2 expression. J Biol Chem;281(34):24171-81. 2006 Aug 25. [PMID: 16815840] (WB, Human) WB Human
Chen, H et al. Hypoxic stress induces dimethylated histone H3 lysine 9 through histone methyltransferase G9a in mammalian cells. Cancer Res;66(18):9009-16. 2006 Sep 15. [PMID: 16982742] (WB, Human) WB Human
Lukashev D, Klebanov B, Kojima H et al. Cutting edge: hypoxia-inducible factor 1alpha and its activation-inducible short isoform I.1 negatively regulate functions of CD4+ and CD8+ T lymphocytes. J Immunol. 177(8):4962-5. 2006 Oct 15. [PMID: 17015677] (WB, Mouse) WB Mouse
Kang, S-H et al. Antitumor effect of 2-methoxyestradiol in a rat orthotopic brain tumor model. Cancer Res;66(24):11991-7. 2006 Dec 15. [PMID: 17178898] (IHC-P, Rat) IHC-P Rat
LaRusch GA, Jackson MW, Dunbar JD et al. Nutlin3 blocks vascular endothelial growth factor induction by preventing the interaction between hypoxia inducible factor 1alpha and Hdm2. Cancer Res;67(2):450-4. 2007 Jan 15. [PMID: 17234751] (IP, Human) IP Human
Liu, Y et al. Calcineurin promotes hypoxia-inducible factor 1alpha expression by dephosphorylating RACK1 and blocking RACK1 dimerization. J Biol Chem;282(51):37064-73. 2007 Dec 21. [PMID: 17965024] (WB, Human) WB Human
Zhang H, Bosch-Marce M, Shimoda LA et al. Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia. J Biol Chem;283(16):10892-903. 2008 Apr 18. [PMID: 18281291] (WB, Mouse) WB Mouse
Moser C et al. ENMD-1198, a novel tubulin-binding agent reduces HIF-1alpha and STAT3 activity in human hepatocellular carcinoma(HCC) cells, and inhibits growth and vascularization in vivo. BMC Cancer;8:206. 2008 Jul 23. [PMID: 18651980] (WB, Human) WB Human
Zhang H, Qian DZ, Tan YS et al. Digoxin and other cardiac glycosides inhibit HIF-1alpha synthesis and block tumor growth. Proc Natl Acad Sci U S A;105(50):19579-86. 2008 Dec 16. [PMID: 19020076] (WB, Human) WB Human
Wong W, Goehring AS, Kapiloff MS et al. mAKAP compartmentalizes oxygen-dependent control of HIF-1alpha. Sci Signal;1(51):ra18. 2008 Dec 23. [PMID: 19109240] (WB, IP, Human) IP, WB Human
Rezvani HR, Mahfouf W, Ali N, Chemin C et al. Hypoxia-inducible factor-1{alpha} regulates the expression of nucleotide excision repair proteins in keratinocytes. Nucleic Acids Res. 2009 Nov 24. [PMID: 19934262] (GS, ChIP, Human) ChIP, GS Human
Regazzetti C, Bost F, Le Marchand-Brustel Y et al. Insulin induces REDD1 expression through hypoxia-inducible factor 1 activation in adipocytes. J Biol Chem;285(8):5157-64. 2010 Feb 19. [PMID: 19996311] (WB, Mouse) WB Mouse
Tanaka T, Wakamatsu T, Daijo H et al. Persisting mild hypothermia suppresses hypoxia-inducible factor 1{alpha} protein synthesis and HIF-1-mediated gene expression. Am J Physiol Regul Integr Comp Physiol. 2009 Dec 30. [PMID: 20042684] (ELISA, Mouse) ELISA Mouse
Evens AM, Sehn LH, Farinha P et al. Hypoxia-inducible factor-1 {alpha} expression predicts superior survival in patients with diffuse large B-cell lymphoma treated with R-CHOP. J Clin Oncol;28(6):1017-24. 2010 Feb 20. [PMID: 20048181] (IHC, Human) IHC Human
Chen H, Kluz T, Zhang R, Costa M. Hypoxia and Nickel inhibit histone demethylase JMJD1A and repress Spry2 expression in human bronchial epithelial BEAS-2B cells. Carcinogenesis. 2010 Sep 29. [PMID: 20881000] (ChIP, Human) ChIP Human
Lunde IG, Anton SL, Bruusgaard JC, Rana ZA, Ellefsen S, Gundersen K. Hypoxia inducible factor 1 links fast-patterned muscle activity and fast muscle phenotype in rats. J Physiol;589(Pt 6):1443-54. 2011 Mar 15. [PMID: 21262877] (WB, Human, Rat) WB Human, Rat
Chaudhuri S, McCullough SS, Hennings L et al. Acetaminophen hepatotoxicity and HIF-1alpha induction in mice occurs without hypoxia. Toxicol Appl Pharmacol. 2011 Feb 10. [PMID: 21316383] (WB, Mouse) WB Mouse
Leh S, Hultstrom M, Rosenberger C, Iversen BM. Afferent arteriolopathy and glomerular collapse but not segmental sclerosis induce tubular atrophy in old spontaneously hypertensive rats. Virchows Arch. 2011 Jun 10. [PMID: 21660521] (IHC-P, Rat) IHC-P Rat
Nonoguchi N, Miyatake S-I, Fukumoto M et al. The distribution of vascular endothelial growth factor-producing cells in clinical radiation necrosis of the brain: pathological consideration of their potential roles. J Neurooncol. 2011 Jun 18. [PMID: 21688077] (IHC, Human) IHC Human
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Jeong, C-H et al. Hypoxia-inducible factor-1 alpha inhibits self-renewal of mouse embryonic stem cells in Vitro via negative regulation of the leukemia inhibitory factor-STAT3 pathway. J. Biol. Chem. 282(18): 13672-13679. 2007 [PMID: 17360716] (IHC, Mouse) IHC Mouse
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Baek, JH et al. Spermidine/spermine-N1-acetyltransferase 2 is an essential component of the ubiquitin ligase complex that regulates hypoxia-inducible factor 1alpha. J. Biol. Chem.82(32): 23572-23580. 2007 [PMID: 17558023] (IP, WB, Human) IP, WB Human
Baek, JH et al. Spermidine/spermine N(1)-acetyltransferase-1 binds to hypoxia-inducible factor-1alpha (HIF-1alpha) and RACK1 and promotes ubiquitination and degradation of HIF-1alpha. J. Biol. Chem. 282(46): 33358-33366. 2007 [PMID: 17875644] (WB, Human) WB Human
Higgins, D et al. Hypoxia promotes fibrogenesis in vivo via HIF-1 stimulation of epithelial-to-mesenchymal transition. J Clin Invest; 117(12): 3810-3820. doi:10.1172/JCI30487. 2007 [PMID: 18037992] (IHC, Human) IHC Human
Engebretsen, B et al. Acute Hypobaric Hypoxia (5486 m) Induces greater Pulmonary HIF-1 Activation in Hilltop Compared to Madison Rats. High Altitude Medicine Biology; 8(4): 312-321. 2007 [PMID: 18081507] (Rat) Rat
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Champion KJ, Guinea M, Dammai V et al. Endothelial Function of von Hippel-Lindau Tumor Suppressor Gene: Control of Fibroblast Growth Factor Receptor Signaling. Cancer Res;68(12):4649-4657. 2008 [PMID: 18559510] (WB, Human) WB Human
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Yang G, Sun Q, Teng Y et al. PTEN deficiency causes dyschondroplasia in mice by enhanced hypoxia-inducible factor 1{alpha} signaling endoplasmic reticulum stress. Development;135(21):3587-3597. 2008 [PMID: 18832389] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Alam H, Weck J, Maizels E et al. Role of the Phosphatidylinositol-3-Kinase Extracellular Regulated Kinase Pathways in the Induction of Hypoxia-Inducible Factor (HIF)-1 Activity the HIF-1 Target Vascular Endothelial Growth Factor in Ovarian Granulosa Cells in Response to Follicle-Stimulating Hormone. 150(2):915-928. 2009 [PMID: 18845636] (ChIP, Rat) ChIP Rat
Lysiak JJ, Kirby JL, Tremblay JJ et al. Hypoxia-Inducible Factor-1{alpha} Is Constitutively Expressed in Murine Leydig Cells Regulates 3{beta}-Hydroxysteroid Dehydrogenase Type 1 Promoter Activity. J rol;30(2):146-156. 2009 [PMID: 18930903] (GS, Mouse) GS Mouse
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Ngan B-Y, Forte V, Campisi P. Molecular Angiogenic Signaling in Angiofibromas After Embolization: Implications for Therapy. Arch Otolaryngol Head Neck Surg;134(11):1170-1176. 2008 [PMID: 19015446] (IHC-P, Human) IHC-P Human
Gilbert JS, Gilbert SAB, Arany M et al. Hypertension Produced by Placental Ischemia in Pregnant Rats Is Associated With Increased Soluble Endoglin Expression. Hypertension;53(2):399-403. 2009 [PMID: 19075097] (WB, Rat) WB Rat
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Tyagi A, Singh RP, Ramasamy K et al. Growth Inhibition Regression of Lung Tumors by Silibinin: Modulation of Angiogenesis by Macrophage-Associated Cytokines Nuclear Factor-{kappa}B Signal Transducers Activators of Transcription 3. Cancer Prevention Research;2(1):74-83. 2009 [PMID: 19139021] (WB, IHC, Mouse) IHC, WB Mouse
Osorio-Fuentealba C, Valdes JA, Riquelme D et al. Hypoxia stimulates via separate pathways ERK phosphorylation NF-{kappa}B activation in skeletal muscle cells in primary culture. J Appl Physiol;106(4):1301-1310. 2009 [PMID: 19179647] (WB, Rat) WB Rat
Lomb DJ, Desouza LA, Franklin JL et al. Prolyl Hydroxylase Inhibitors Depend on Extracellular Glucose Hypoxia-Inducible Factor (HIF)-2{alpha} to Inhibit Cell Death Caused by Nerve Growth Factor (NGF) Deprivation: Evidence that HIF-2{alpha} Has a Role in NGF-Promoted Survival of Sympathetic Neurons. Mol Pharmacol;75(5):1198-1209. 2009 [PMID: 19204094] (WB, Primate) WB Primate
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Klatte T, Seligson DB, LaRochelle J et al. Molecular Signatures of Localized Clear Cell Renal Cell Carcinoma to Predict Disease-Free Survival after Nephrectomy. Cancer Epidemiol Biomarkers Prev;18(3):894-900. 2009 [PMID: 19240241] (IHC, Human) IHC Human
Zhu K, Chan W, Heymach J et al. Control of HIF-1{alpha} Expression by eIF2{alpha} Phosphorylation-Mediated Translational Repression. Cancer Res;69(5):1836-1843. 2009 [PMID: 19244104] (WB, Human) WB Human
Amann T, Maegdefrau U, Hartmann A et al. GLUT1 Expression Is Increased in Hepatocellular Carcinoma Promotes Tumorigenesis. Am J Pathol;174(4):1544-1552. 2009 [PMID: 19286567] (WB, Human) WB Human
Kim J, Bagchi IC, Bagchi MK. Signaling by Hypoxia-Inducible Factors is Critical for Ovulation In Mice. Endocrinology 2009:en-0948. 2008 [PMID: 19325003] (WB, Mouse) WB Mouse
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Rhoads RP, Johnson RM, Rathbone CR et al. Satellite cell-mediated angiogenesis in vitro coincides with a functional hypoxia-inducible factor pathway. Am J Physiol Cell Physiol;296(6):C1321-1328. 2009 [PMID: 19386789] (IHC, Rat) IHC Rat
Torii S, Kobayashi K, Takahashi M et al. Magnesium Deficiency Causes Loss of Response to Intermittent Hypoxia in Paraganglion Cells. J Biol Chem;284(28):19077-19089. 2009 [PMID: 19433582] (WB, Rat, Human) WB Rat, Human
Guma M, Rius J, Duong-Polk KX et al. Genetic pharmacological inhibition of JNK ameliorates hypoxia-induced retinopathy through interference with VEGF expression. PNAS;106(21):8760-8765. 2009 [PMID: 19433784] (WB, Mouse) WB Mouse
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Shackelford DB, Vasquez DS, Corbeil J et al. mTOR HIF-1{alpha}-mediated tumor metabolism in an LKB1 mouse model of Peutz-Jeghers syndrome. PNAS;106(27):11137-11142. 2009 [PMID: 19541609] (IHC, Mouse) IHC Mouse
Loinard C, Ginouves A, Vilar J et al. Inhibition of Prolyl Hydroxylase Domain Proteins Promotes Therapeutic Revascularization. Circulation;120(1):50-59. 2009 [PMID: 19546390] (WB, Mouse) WB Mouse
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Doroudgar S, Thuerauf DJ, Marcinko MC et al. Ischemia Activates the ATF6 Branch of the Endoplasmic Reticulum Stress Response. J Biol Chem;284(43):29735-29745. 2009 [PMID: 19622751] (WB, Rat) WB Rat
Mahimainathan L, Ghosh-Choudhury N, Venkatesan B et al. TSC2 Deficiency Increases PTEN via HIF1{alpha}. J Biol Chem;284(41):27790-27798. 2009 [PMID: 19648120] (WB, Mouse) WB Mouse
Chen A, Cuevas I, Kenny PA et al. Endothelial Cell Migration Vascular Endothelial Growth Factor Expression Are the Result of Loss of Breast Tissue Polarity. Cancer Res;69(16):6721-6729. 2009 [PMID: 19654314] (WB, Human) WB Human
Thangarajah H, Yao D, Chang EI et al. The molecular basis for impaired hypoxia-induced VEGF expression in diabetic tissues. PNAS;106(32):13505-13510. 2009 [PMID: 19666581] (WB, Human) WB Human
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Tsuruda T, Hatakeyama K, Masuyama H et al. Pharmacological Stimulation of Soluble Guanylate Cyclase Modulates Hypoxia Inducible Factor-1{alpha} in Rat Heart. Am J Physiol Heart Circ Physiol :00503. 2009 [PMID: 19684186] (IHC, WB, Rat) IHC, WB Rat
Lee K, Zhang H, Qian DZ et al. Acriflavine inhibits HIF-1 dimerization, tumor growth, and vascularization. PNAS;106(42):17910-17915. 2009 [PMID: 19805192] (ChIP, Human) ChIP Human
Hsieh C-H, Shyu W-C, Chiang C-Y et al. NADPH oxidase subunit 4-mediated reactive oxygen species contribute to cycling hypoxia-promoted tumor progression in glioblastoma multiforme. PLoS ONE;6(9). 2011 [PMID: 21935366] (WB, Human) WB Human
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Lee JP, Yang SH, Lee HY, Kim B, Cho JY, Paik JH, Oh YJ, Kim DK, Lim CS, Kim YS. Soluble epoxide hydrolase activity determines the severity of ischemia-reperfusion injury in kidney. PLoS One;7(5):e37075. 2012 [PMID: 22590647] (ICC/IF, Human) ICC/IF Human
Norregaard R, Bodker T, Jensen BL et al. Increased renal adrenomedullin expression in rats with ureteral obstruction. Am J Physiol Regulatory Integrative Comp Physiol 9;296(1):R185-192. 200 [PMID: 18945953] (WB, Rat) WB Rat
Yeh S-H, Hung J-J, Gean P-W et al. Hypoxia-Inducible Factor-1{alpha} Protects Cultured Cortical Neurons from Lipopolysaccharide-Induced Cell Death via Regulation of NR1 Expression. J Neurosci 8;28(52):14259-14270. 200 [PMID: 19109507] (WB, ICC/IF, Rat) ICC/IF, WB Rat
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Klatte T, Pantuck AJ, Said JW et al. Cytogenetic Molecular Tumor Profiling for Type 1 Type 2 Papillary Renal Cell Carcinoma. 2009;15(4):1162-1169. [PMID: 19228721] (IHC, Human) IHC Human
Tsai Y-W, Yang Y-R, Wang PS, Wang R-Y. Intermittent Hypoxia after Transient Focal Ischemia Induces Hippocampal Neurogenesis and c-Fos Expression and Reverses Spatial Memory Deficits in Rats. PLoS ONE 20116(8). [PMID: 21887361] (WB, Rat) WB Rat
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Chaturvedi P, Gilkes DM, Wong CC et al. Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis J Clin Invest 2013 Jan 2 [PMID: 23318994] (WB, Human) WB Human
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Hsieh YL, Yang SA, Yang CC, Chou LW. Dry needling at myofascial trigger spots of rabbit skeletal muscles modulates the biochemicals associated with pain, inflammation, and hypoxia. Evid Based Complement Alternat Med 2012 [PMID: 23346198] (WB, Rabbit) WB Rabbit
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Kawedia JD, Yang F, Sartor MA et al. Hypoxia and Hypoxia Mimetics Decrease Aquaporin 5 (AQP5) Expression through Both Hypoxia Inducible Factor-1alpha and Proteasome-Mediated Pathways. PLoS One 2013 [PMID: 23469202] (WB, Human) WB Human
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Wu C, Zhou Y, Chang J, Xiao Y. Delivery of dimethyloxallyl glycine in mesoporous bioactive glass scaffolds to improve angiogenesis and osteogenesis of human bone marrow stromal cells. Acta Biomater 2013 Jun 26 [PMID: 23811216] (WB, Human) WB Human
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Xie L, Collins JF. Transcription Factors Sp1 and Hif2alpha Mediate Induction of the Copper-Transporting ATPase (Atp7a) Gene in Intestinal Epithelial Cells During Hypoxia. J Biol Chem 2013 Jun 28 [PMID: 23814049] (WB, Rat) WB Rat
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Jung JH, Im S, Jung ES, Kang CS. Clinicopathological Implications of the Expression of Hypoxia-related Proteins in Gastric Cancer. Int J Med Sci 2013 Jul 31 [PMID: 23935399] (IHC-P, Human) IHC-P Human
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Sun YY, Lin SH, Lin HC et al. Cell Type-Specific Dependency on the PI3K/Akt Signaling Pathway for the Endogenous Epo and VEGF Induction by Baicalein in Neurons versus Astrocytes. PLoS One 2013 Jul 19 [PMID: 23904909] (ChIP, Rat) ChIP Rat
Belloli S, Brioschi A, Politi LS et al. Characterization of biological features of a rat F98 GBM model: A PET-MRI study with [(18)F]FAZA and [(18)F]FDG. Nucl Med Biol 2013 Aug [PMID: 23915802] (IHC-P, Rat) IHC-P Rat
Ma Z, Moruzzi N, Catrina SB et al. Preconditioning with Associated Blocking of Ca2+ Inflow Alleviates Hypoxia-Induced Damage to Pancreatic beta-Cells. PLoS One 2013 Jul 25 [PMID: 23935835] (WB, Rat) WB Rat
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Takibuchi G, Imanishi H, Morimoto M et al. Polymorphic mutations in mouse mitochondrial DNA regulate a tumor phenotype. Mitochondrion. 2013 Aug 6 [PMID: 23932994] (WB, Mouse) WB Mouse
Wang SC, Yu CF, Hong JH et al. Radiation Therapy-Induced Tumor Invasiveness Is Associated with SDF-1-Regulated Macrophage Mobilization and Vasculogenesis. PLoS One. 2013 Aug 5 [PMID: 23940516] (WB, Mouse) WB Mouse
Zaccagnini G, Maimone B, Di Stefano V et al. Hypoxia-induced miR-210 modulates tissue response to acute peripheral ischemia. Antioxid Redox Signal. 2013 Aug 10 [PMID: 23931770]
Minoia C, Quero C, Asselti M et al. Changes in angiogenesis and Hypoxia-Inducible Factor-1alpha protein expression in relapsed/refractory indolent non-Hodgkin Lymphomas. Br J Haematol. 2013 Sep 13 [PMID: 24033233] (IHC, Human) IHC Human
Gallaher AM, Das S, Xiao Z et al. Proteomic Screening of Human Targets of Viral microRNAs Reveals Functions Associated with Immune Evasion and Angiogenesis. PLoS Pathog. 2013 Sep [PMID: 24039573] (WB, Human) WB Human
Eckle T, Brodsky K, Bonney M et al. HIF1A Reduces Acute Lung Injury by Optimizing Carbohydrate Metabolism in the Alveolar Epithelium. PLoS Biol. 2013 Sep [PMID: 24086109] (WB, IHC-P, Human, Mouse) WB, IHC-P Human, Mouse
Miyauchi Y, Sato Y, Kobayashi T et al. HIF1alpha is required for osteoclast activation by estrogen deficiency in postmenopausal osteoporosis. Proc Natl Acad Sci U S A. 2013 Oct 8 [PMID: 24023068] (WB, IHC-P, ICC/IF, Mouse) ICC/IF, WB, IHC-P Mouse
Erler JT, Cawthorne CJ, Williams KJ et al. Hypoxia-mediated down-regulation of Bid and Bax in tumors occurs via hypoxia-inducible factor 1-dependent and-independent mechanisms and contributes to drug Mol Cell Biol. 2004 Apr [PMID: 15024076] (WB, Mouse, Human) WB Mouse, Human
Zhang L, Xu J, Sun N et al. The Presence of Adenosine A2a Receptor in Thyrocytes and Its Involvement in Graves' IgG-Induced VEGF Expression. Endocrinology. 2013 Sep 30 [PMID: 24080368] (WB, IHC-P, ICC/IF, Mouse, Human) ICC/IF, WB, IHC-P Mouse, Human
Wall AM, Corcoran AE, O'Halloran KD, O'Connor JJ. Effects of prolyl-hydroxylase inhibition and chronic intermittent hypoxia on synaptic transmission and plasticity in the rat CA1 and dentate gyrus. Neurobiol Dis. 2013 Sep 17 [PMID: 24055213] (WB, Rat) WB Rat
CJ Hu, A Sataur, L Wang et al. The N-terminal transactivation domain confers target gene specificity of hypoxia-inducible factors HIF-1a and HIF-2a. Mol Biol Cell. 2007 Nov [PMID: 17804822]
Tan JT, Prosser HC, Vanags LZ et al. High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1alpha. FASEB J. 2013 Sep 10 [PMID: 24022405] (WB, Human) WB Human
Ding G, Huang G, Liu HD et al. MiR-199a suppresses the hypoxia-induced proliferation of non-small cell lung cancer cells through targeting HIF1alpha. Mol Cell Biochem. 2013 Sep 11 [PMID: 24022342] (WB, IHC, Human) IHC, WB Human
Hu P, Chung LW, Berel D et al. Convergent RANK- and c-Met-Mediated Signaling Components Predict Survival of Patients with Prostate Cancer: An Interracial Comparative Study. PLoS One. 2013 Sep 16 [PMID: 24066029] (Human) Human
Cowburn AS, Alexander LE, Southwood M et al. Epidermal Deletion of HIF-2alpha Stimulates Wound Closure. J Invest Dermatol. 2013 Sep 13 [PMID: 24037341] (IHC-P, Mouse) IHC-P Mouse
Madej JA, Madej JP, Dziegiel P et al. Expression of hypoxia-inducible factor-1alpha and vascular density in mammary adenomas and adenocarcinomas in bitches. Acta Vet Scand. 2013 Oct 24 [PMID: 24153191] (IHC-P, Canine) IHC-P Canine
Zhang Y, Marsboom G, Toth PT, Rehman J. Mitochondrial respiration regulates adipogenic differentiation of Human mesenchymal stem cells. PLoS One. 2013 Oct 18 [PMID: 24204740] (ICC/IF, Human) ICC/IF Human
Koide N, Odkhuu E, Naiki Y et al. Augmentation of LPS-induced vascular endothelial cell growth factor production in macrophages by transforming growth factor-beta1. Innate Immun. 2013 Nov 13 [PMID: 24225655] (WB, Mouse) WB Mouse
Ma Z, Moruzzi N, Catrina SB et al. Hyperoxia inhibits glucose-induced insulin secretion and mitochondrial metabolism in rat pancreatic islets. Biochem Biophys Res Commun. 2013 Dec 2 [PMID: 24299957] (WB, Rat) WB Rat
Liu X, Tu Y, Zhang L et al. Prolyl Hydroxylase Inhibitors Protect from the Bone Loss in Ovariectomy Rats by Increasing Bone Vascularity. Cell Biochem Biophys. 2013 Nov 16 [PMID: 24242187] (WB, Rat) WB Rat
Ganapathy S, Xiao S, Yang M et al. A low-dose arsenic induced p53-mediated metabolic mechanism of radiotherapy protection. J Biol Chem 2014 Jan 3 [PMID: 24391088] (ICC/IF, Human) ICC/IF Human
Chen TM, Shih YH, Tseng JT et al. Overexpression of FGF9 in colon cancer cells is mediated by hypoxia-induced translational activation. Nucleic Acids Res 2013 Dec 10 [PMID: 24334956] (IHC, Human) IHC Human
Chen Z, He X, Xia W et al. Prognostic Value and Clinicopathological Differences of HIFs in Colorectal Cancer: Evidence from Meta-Analysis. PLoS One 2013 Dec 6 [PMID: 24324596] (IHC, Human) IHC Human
An X, Xu G, Yang L et al. Expression of hypoxia-inducible factor-1alpha, vascular endothelial growth factor and prolyl hydroxylase domain protein 2 in cutaneous squamous cell carcinoma and precursor lesions and their relationship with histological stages and clinical features. J Dermatol 2013 Dec 20 [PMID: 24354513] (IHC-P, Human) IHC-P Human
Miyazaki S, Kikuchi H, Iino I et al. Anti-VEGF antibody therapy induces tumor hypoxia and stanniocalcin 2 expression and potentiates growth of human colon cancer xenografts. Int J Cancer 2013 Dec 20 [PMID: 24375080] (IHC-P, Human) IHC-P Human
Garofalo C, Capristo M, Manara MC et al. Metformin as an Adjuvant Drug against Pediatric Sarcomas: Hypoxia Limits Therapeutic Effects of the Drug. PLoS One 2013 Dec 31 [PMID: 24391834] (IHC-P, Human) IHC-P Human
Jensen RL, Mumert ML, Gillespie DL et al. Preoperative dynamic contrast-enhanced MRI correlates with molecular markers of hypoxia and vascularity in specific areas of intratumoral microenvironment and is predictive of patient outcome. Neuro Oncol 2013 Dec 4 [PMID: 24305704] (IHC-P, Human) IHC-P Human
Fernandes BF, Coates J, Odashiro AN et al. Hypoxia-Inducible Factor-1alpha and its Role in the Proliferation of Retinoblastoma Cells. Pathol Oncol Res 2013 Dec 14 [PMID: 24338218] (WB, Human) WB Human
Watson CJ, Collier P, Tea I et al. Hypoxia-induced epigenetic modifications are associated with cardiac tissue fibrosis and the development of a myofibroblast-like phenotype. Hum Mol Genet 2013 Dec 16 [PMID: 24301681] (WB, Human) WB Human
Ji X, Wang H, Zhu J et al. Knockdown of Nrf2 suppresses glioblastoma angiogenesis by inhibiting hypoxia-induced activation of HIF-1alpha. Int J Cancer 2013 Dec 26 [PMID: 24374745] (WB, Human) WB Human
Zhang C, Yang X, Zhang Q et al. Berberine radiosensitizes human nasopharyngeal carcinoma by suppressing hypoxia-inducible factor-1alpha expression. Acta Otolaryngol 2014 Feb [PMID: 24325635] (WB, Human) WB Human
Fujimura A, Michiue H, Cheng Y et al. Cyclin g2 promotes hypoxia-driven local invasion of glioblastoma by orchestrating cytoskeletal dynamics. Neoplasia 2013 Nov [PMID: 24339739] (WB, ICC/IF, Human) ICC/IF, WB Human
Park HJ, Kim MK, Kim SR. Hypoxia Regulates the Expression of the Neuromedin B Receptor through a Mechanism Dependent on Hypoxia-Inducible Factor-1A. PLoS One 2013 Dec 9 [PMID: 24349381] (WB, ICC/IF, IHC-P, ChIP, Human) ChIP, ICC/IF, WB, IHC-P Human
Takano N, Peng YJ, Kumar GK et al. Hypoxia-inducible factors regulate human and rat cystathionine B-synthase gene expression. Biochem J 2013 Dec 12 [PMID: 24328859] (WB, ChIP, Human, Rat) ChIP, WB Human, Rat
Kawakami T, Yoshimi M, Kadota Y et al. Prolonged endoplasmic reticulum stress alters placental morphology and causes low birth weight. Toxicol Appl Pharmacol 2013 Dec 23 [PMID: 24370435] (IHC-P, Mouse) IHC-P Mouse
Ouyang W, Torigoe C, Fang H et al. Anthrax lethal toxin inhibits translation of hypoxia inducible factor 1alpha and causes decreased tolerance to hypoxic stress. J Biol Chem 2013 Dec 23 [PMID: 24366872] (WB, Mouse) WB Mouse
Kind KL, Tam KK, Banwell KM et al. Oxygen-regulated gene expression in murine cumulus cells. Reprod Fertil Dev 2014 Jan 6 [PMID: 24388334] (WB, Mouse) WB Mouse
Popescu CR, Sutherland MR, Cloutier A et al. Hyperoxia Exposure Impairs Nephrogenesis in the Neonatal Rat: Role of HIF-1alpha. PLoS One 2013 Dec 17 [PMID: 24358181] (IHC-P, Rat) IHC-P Rat
Park EY, Lee CH, Lee EK et al. HNF4alpha contributes to glucose formation in aged rat hepatocytes. Exp Gerontol 2013 Dec [PMID: 24177414] (WB, Rat) WB Rat
Engelhardt S, Al-Ahmad AJ, Gassmann M, Ogunshola OO. Hypoxia Selectively Disrupts Brain Microvascular Endothelial Tight Junction Complexes Through A Hypoxia-Inducible Factor-1 (HIF-1) Dependent Mechanism. J Cell Physiol 2013 Dec 25 [PMID: 24375098] (WB, Rat) WB Rat
Ma Z, Moruzzi N, Catrina SB et al. Hyperoxia inhibits glucose-induced insulin secretion and mitochondrial metabolism in rat pancreatic islets. Biochem Biophys Res Commun 2014 Jan 3 [PMID: 24299957] (WB, Rat) WB Rat
Zhao W, Chang C, Cui Y et al. Steroid Receptor Coactivator-3 Regulates Glucose Metabolism in Bladder Cancer Cells through Coactivation of Hypoxia Inducible-Factor 1 alpha. J. Biol. Chem. 2014 Mar 3 [PMID: 24584933] (ChIP, Human) ChIP Human
Lall R, Ganapathy S, Yang M et al. Low-dose radiation exposure induces a HIF-1-mediated adaptive and protective metabolic response. Cell Death Differ. 2014 Feb 28 [PMID: 24583639] (ICC/IF, Human) ICC/IF Human
Mateo J, Izquierdo-Garcia D, Badimon JJ, Fuster V. Noninvasive Assessment of Hypoxia in Rabbit Advanced Atherosclerosis Using 18F-fluoromisonidazole PET Imaging. Circ Cardiovasc Imaging 2014 Feb 10 [PMID: 24508668] (IHC-P, Human) IHC-P Human
Chen Z, Liu X, Mei Z, Xiao W. EAF2 Suppresses Hypoxia-Induced Factor 1 alpha Transcriptional Activity by Disrupting Its Interaction with Coactivator CBP/p300. Mol. Cell. Biol. 2014 Mar 1 [PMID: 24421387] (WB, Human) WB Human
Bertozzi D, Marinello J, Manzo SG et al. The natural inhibitor of DNA topoisomerase I, camptothecin, modulates HIF-1 alpha activity by changing miR expression patterns in human cancer cells. Mol. Cancer Ther. 2014 Jan 1 [PMID: 24252850] (WB, Human) WB Human
White NM, Masui O, Newsted D et al. Galectin-1 has potential prognostic significance and is implicated in clear cell renal cell carcinoma progression through the HIF/mTOR signaling axis. Br. J. Cancer 2014 Mar 5 [PMID: 24496460] (WB, Human) WB Human
Huang SM, Chen TS, Chiu CM et al. GDNF increases cell motility in human colon cancer through VEGF-VEGFR1 interaction. Endocr. Relat. Cancer 2014 Feb 1 [PMID: 24165321] (WB, Human) WB Human
Pantel A, Teixeira A, Haddad E et al. Direct type I IFN but not MDA5/TLR3 activation of dendritic cells is required for maturation and metabolic shift to glycolysis after poly IC stimulation. PLoS Biol. 2014 Jan 1 [PMID: 24409099] (FLOW, Mouse) FLOW Mouse
Cheng YL, Park JS, Manzanero S et al. Evidence that collaboration between HIF-1 alpha and Notch-1 promotes neuronal cell death in ischemic stroke. Neurobiol. Dis. 2014 Feb 1 [PMID: 24141018] (WB, ICC/IF, Mouse) ICC/IF, WB Mouse
Weisel FC, Kloepping C, Pichl A et al. Impact of S-Adenosylmethionine Decarboxylase 1 in Pulmonary Vascular Remodeling. Circulation 2014 Jan 28 [PMID: 24470481] (ChIP, Mouse, Human) ChIP Mouse, Human
He M, Lu Y, Xu S et al. MiRNA-210 modulates a nickel-induced cellular energy metabolism shift by repressing the iron-sulfur cluster assembly proteins ISCU1/2 in Neuro-2a cells. Cell Death Dis 2/28/2014 [PMID: 24577088] (WB, Mouse) WB Mouse
Li Y, Chen J, Zeng T et al. Expression of HIF-1a and MDR1/P-glycoprotein in refractory mesial temporal lobe epilepsy patients and pharmacoresistant temporal lobe epilepsy rat model kindled by coriaria lactone. Neurol. Sci. 3/4/2014 [PMID: 24590840] (WB, Rat) WB Rat
Huang YH, Lin MH, Wang PC et al. Hypoxia inducible factor 2a/insulin-like growth factor receptor signal loop supports the proliferation and Oct-4 maintenance of mouse germline stem cells. Mol. Hum. Reprod. 3/23/2014 [PMID: 24598112] (WB, Mouse) WB Mouse
Imamura Y, Tomita S, Imanishi M et al. HIF-2a/ARNT complex regulates hair development via induction of p21Waf1/Cip1 and p27Kip1. FASEB J. 3/5/2014 [PMID: 24599965] (WB, Mouse) WB Mouse
Shan F, Li J, Huang QY. HIF-1 Alpha-Induced Up-Regulation of miR-9 Contributes to Phenotypic Modulation in Pulmonary Artery Smooth Muscle Cells During Hypoxia. J. Cell. Physiol. 2/25/2014 [PMID: 24615545] (WB, Rat) WB Rat
Zhao L, Yang YF, Gao YB et al. Upregulation of HIF-1a Via Activation of ERK and PI3K Pathway Mediated Protective Response to Microwave-Induced Mitochondrial Injury in Neuron-Like Cells. Mol. Neurobiol. 3/14/2014 [PMID: 24627260] (WB, Rat) WB Rat
Pan WL, Wong JH, Fang EF et al. Preferential cytotoxicity of the type I ribosome inactivating protein alpha-momorcharin on human nasopharyngeal carcinoma cells under normoxia and hypoxia. Biochem. Pharmacol. 3/14/2014 [PMID: 24637239] (WB, Human) WB Human
Sano M, Sasaki T, Hirakawa S et al. Lymphangiogenesis and angiogenesis in abdominal aortic aneurysm. PLoS ONE 3/21/2014 [PMID: 24651519] (IHC-P, Human) IHC-P Human
Zhang WG, He L, Shi XM et al. Regulation of transplanted mesenchymal stem cells by the lung progenitor niche in rats with chronic obstructive pulmonary disease. Respir. Res. 3/25/2014 [PMID: 24661402] (IHC-P, WB, Rat) WB, IHC-P Rat
Raspaglio G, Petrillo M, Martinelli E et al. Sox9 and Hif-2a regulate TUBB3 gene expression and affect ovarian cancer aggressiveness. Gene 3/21/2014 [PMID: 24661907] (WB, Human) WB Human
Chang ML, Chiu CJ, Shang F, Taylor A. High Glucose Activates ChREBP-Mediated HIF-1a and VEGF Expression in Human RPE Cells Under Normoxia. Adv. Exp. Med. Biol. 3/25/2014 [PMID: 24664750] (WB, Human) WB Human
Zheng J, Song F, Lu SL, Wang XQ. Dynamic Hypoxia in Scar Tissue During Human Hypertrophic Scar Progression. Dermatol Surg 2014 Mar 31 [PMID: 24684437] (IHC-P, Human) IHC-P Human
Kim YS, Lee HA, Lim JY et al. beta-Carotene inhibits neuroblastoma cell invasion and metastasis in vitro and in vivo by decreasing level of hypoxia-inducible factor-1alpha. J. Nutr. Biochem. 2014 Mar 13 [PMID: 24746828] (WB, IHC-P, Human) WB, IHC-P Human
Xu W, Kwon JH, Moon YH et al. Influence of preoperative transcatheter arterial chemoembolization on gene expression in the HIF-1alpha pathway in patients with hepatocellular carcinoma. J. Cancer Res. Clin. Oncol. 2014 May 23 [PMID: 24853275] (IHC-P, Human) IHC-P Human
Arreola A, Cowey CL, Coloff JL et al. HIF1alpha and HIF2alpha Exert Distinct Nutrient Preferences in Renal Cells. PLoS ONE. 2014 Jun 02 [PMID: 24879016] (WB, Mouse) WB Mouse
Xiong Y, Qu Z, Chen N et al. The local corticotropin-releasing hormone receptor 2 signalling pathway partly mediates hypoxia-induced increases in lipolysis via the cAMP-protein kinase A signalling pathway in white adipose tissue. Mol. Cell. Endocrinol. 2014 May 22 [PMID: 24859650] (WB, Rat) WB Rat
Wu JB, Shao C, Li X et al. Near-infrared fluorescence imaging of cancer mediated by tumor hypoxia and HIF1alpha/OATPs signaling axis. Biomaterials. 2014 Jun 20 [PMID: 24957295] (IHC-P, Human)

Details:
Archival formalin-fixed paraffin-embedded/FFPE human prostate cancer/PCa specimens were processed for IHC staining with HIF1 alpha antibody (clone H1alpha67, 1:30 dilution).
IHC-P Human
Yang MY, Chiao MT, Lee HT et al. An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior J. Biomed. Mater. Res. Part B Appl. Biomater. 2014 Jun 26 [PMID: 24966152] (IHC, Human)

Details:
HIF-1 alpha antibody used for IHC -DAB of xenograft tissue of human glioblastoma stem cells/GSCs grown in gelatin foam. Figure 5 f - HIF1 alpha used as an intrinsic marker of tumor hypoxia.
IHC Human
Vorrink SU, Sarsour EH, Olivier AK et al. PCB 126 perturbs hypoxia-induced HIF-1a activity and glucose consumption in human HepG2 cells Exp. Toxicol. Pathol. 2014 Jun 07 [PMID: 24916446] (IHC-P, Rat)

Details:
HIF 1 alpha antibody used for FFPE liver sections from male Sprague-Dawley rats treated or not with PCB 126 (AhR agonist ) - HIER using citrate buffer pH 6.0, Peroxidase quenching with 3% H2O2 for 8 min, blocking with 5% horse serum, 1:50 primary - 2 h incubation at RT, detected via Mouse Envision HRP System Reagent -DAB method. Fig 1 - PCB 126 treated livers had mainly cytoplasmic/decreased nuclear staining compared to control rats.
IHC-P Rat
Privat M, Radosevic-Robin N, Aubel C et al. BRCA1 Induces Major Energetic Metabolism Reprogramming in Breast Cancer Cells. PLoS ONE 2014 Jul 11 [PMID: 25010005] (WB, Human)

Details:
HIF-1 alpha antibody used for WB on hypoxia mimetic CoCl2 treated /not treated SUM1315-BRCA1 and SUM1315-LXSN cell lines which were obtained by stable transfection of SUM1315 human breast cancer cells with an empty LXSN plasmid, or a BRCA1-encoding LXSN plasmid (WB image shown in Figure S1).
WB Human
Kalamida D, Karagounis IV, Giatromanolaki A, Koukourakis MI. Important role of autophagy in endothelial cell response to ionizing radiation. PLoS ONE 2014 Jul 11 [PMID: 25010689] (WB, Human)

Details:
HIF-1 alpha antibody used for WB on lysates of HUVEC/human umbilical vein endothelial cells after exposure to 2 Gy of IR with or without 2 day pre-incubation with SMER28/Small-Molecule Enhancer of Rapamycin-28 (an mTOR-independent inducer of autophagy). Primary antibody was used at 1:500 dilution and the blot images are not shown in the article.
WB Human
Zhou W, Marinescu M, Veltkamp R. Only Very Early Oxygen Therapy Attenuates Posthemorrhagic Edema Formation and Blood-Brain Barrier Disruption in Murine Intracerebral Hemorrhage. Neurocrit Care 2014 Jul 15 [PMID: 25022804] (WB, Mouse)

Details:
HIF-1 alpha antibody used for WB (1:500 dilution) on lysates of serial coronal cryosections of hemorrhagic and nonhemorrhagic hemispheres from C57BL/6 male mice 3d after inducing Intracerebral Hemorrhage/ ICH (Fig 6B).
WB Mouse
Kasper LH, Qu C, Obenauer JC et al. Genome-wide and single-cell analyses reveal a context dependent relationship between CBP recruitment and gene expression. Nucleic Acids Res. 2014 Sep 23 [PMID: 25249627] (ChIP, Mouse) ChIP Mouse
Vorrink SU. Regulatory crosstalk and interference between the PCB 126 stimulated AHR and hypoxia stimulated HIF-1a signaling pathways. 2014 (IHC-P, Rat)

Details:
Mouse monoclonal anti-HIF-1 alpha antibody used for IHC on paraffin-embedded livers from untreated and PCB 126 treated rats at a dilution of 1:50, incubated for 2 hours at room temperature (Figure 3-1)
IHC-P Rat
Gu J, Fan Y, Liu X et al. SENP1 protects against myocardial ischaemia/reperfusion injury via a HIF1a-dependent pathway. Cardiovasc. Res. 2014 Jul 31 [PMID: 25082844] (WB, IP, Rat) IP, WB Rat
Huang T, Huang W, Zhang Z et al. Hypoxia-inducible factor-1a upregulation in microglia following hypoxia protects against ischemia-induced cerebral infarction. Neuroreport. 2014 Oct 01 [PMID: 25089804]
Lee HS, Kim KS, Lim HS et al. Priming Wharton's jelly-derived mesenchymal stromal/stem cells with ROCK inhibitor improves recovery in an intracerebral hemorrhage model. J Cell. Biochem. 2014 Sep 04 [PMID: 25185536]

Product General Protocols

View specific protocols for HIF-1 alpha Antibody (NB100-105): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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FAQs for HIF-1 alpha (17)

  1. Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
    • All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, as desired by you, I am suggesting you four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products has been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449 ) (cited in at least 31 peer reviewed publications).
  2. What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
    • The theoretical molecular weight of HIF 1-alpha is ~93kDa, however, you will likely see a band between 100-120kDa due to phosphorylation.
  3. Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
    • HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
  4. I would like to know, does it exist path for detection HIF 1 in the venous blood before and after revascularization of the leg?
    • We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
  5. Do you have any suggested IHC Protocol for this antibody? We had been trying to validate protocols from other reserach studies, but failed to get good results. Background staining seems to be the problem.
    • Yes, we have a specific IHC protocol for NB100-105. It is located on the datasheet under the "Protocols & FAQs" tab. We also have over 300 publications that utilize this product. If you need help finding a specific paper or if you would like us to help troubleshoot your experiment, please contact Technical Support at technical@novusbio.com.
  6. Has anyone used this antibody for supershft in EMSA?
    • Yes, this antibody is useful for EMSA and has been documented in couple of recent research publications. You may refer to the following paper for details: Kong D, et al. Echinomycin, a small-molecule inhibitor of hypoxia-inducible factor-1 DNA-binding activity. Cancer Res. 2005 Oct 1;65(19):9047-55 [PMID: 16204079].
  7. What is the difference in the following two HIF-1 alpha antibodies? Catalog # NB100-105 and NB100-134
    • NB100-105 is a mouse monoclonal antibody, whereas NB100-134 is a rabbit polyclonal. They both use the same immunogen.
  8. I am working on a WB using the antibody against mouse HIF1a and HIF2a (catalog numbers NB100-105 and NB100-122). I tried several anti-mouse and anti-rabbit HRP secondary antibodies (from another company) and found they gave different patterns. Please provide the secondary antibody info which was used in the WB showed on the datasheet.
    • We use catalog numbers NB7570 and NB7183 as our secondary antibodies for testing these antibodies in WB.
  9. What is the difference between NB100-105 and NB100-123?
    • NB100-105 and NB100-123 are similar as far as immunogens and clones are concerned, however, they are different with respect to their purification process, concentrations and recommended dilutions. NB100-123 is Protein A purified IgG2b with a concentration of 1.5 mg/ml, whereas NB100-105 is Protein G purified IgG2b with 4.0 mg/ml concentration. Another difference is the recommended dilutions (because of the differences in their concentration).
  10. Can I get a sample of the HIF1A antibody (NB100-105)?
    • We do offer this antibody is a Sample Size, which contains 0.025ml of antibody. The sample size catalog number is NB100-105SS.
  11. Does NB100-105 work well after stored in -20 degrees Celsius for two days? I forget to read the datasheet and put it in -20 when I got it.
    • In our experience with this product, freezing can damage the function of the antibody. Since it was only frozen for two days, your product might be fine. However, you may want to test it in a preliminary assay to be sure it is still functioning.
  12. I did staining of 150uM Cobalt chloride induced 3T12 for 24 hours using this HIF1alpha antibody. I used a 1:50 dilution for one hour at room temperature. Should I do an overnight incubation?
    • We do recommend incubating samples overnight at 4 degrees Celsius for the best results.
  13. Is there a product available to be used as the blocking peptide of NB100-105?
    • We unfortunately do not have a blocking peptide for NB100-105. It was raised against a fusion protein. We also unfortunately do not sell the protein.
  14. Could you, advice if I can use NBP1-96969 Ab as a control Ab for HIF1a for ChIP asay. I have a problem that normal rabbit IgG that usually are provided in the ChIP kits show higher binding compare to NB100-105 HIF1A Ab. Could you suggest if it is better to use Mouse IgG 2b Isotype control in this case or normal rabbit IgG should work as well? NBP1-96969 Ab are not validated for ChIP, please, let me know if there is something better?
    • Yes, it would be reasonable to use a mouse IgG2b control for NB100-105 (HIF1 alpha) in ChIP assay. The control provided in the ChIP kit is recommended as a control for the histone antibodies (which are rabbit polyclonal). NBP1-96969 would be a good choice for control for the mouse monoclonal NB100-105. It has not yet been validated for ChIP, but it has been validated for use in IP, so I think it will be a good choice for control.
  15. Hi, hope everthing goes well with you Here a customer intends to buy our HIF-1 alpha Antibody (H1alpha67), the link is <a href=http://www.novusbio.com/HIF-1-alpha-Antibody-H1alpha67_NB100-105.html>http://www.novusbio.com/HIF-1-alpha-Antibody-H1alpha67_NB100-105.html</a>.Since HIF-1 is nuclear protein, he wonders why there is obvious stainning in the cytoplasm, which is displayed in the IHC image on the product website. Can you so kind to help him and me?
    • HIF1-alpha is found at very low levels in the cytoplasm under normoxic conditions. During hypoxia, HIF1-alpha is stabilized and translocates to the nucleus to act as a transcription factor. You would therefore expect to see nuclear staining in your hypoxic samples, but may also observe faint cytoplasmic staining in relation to degraded HIF in the samples. As you know the HIF proteins are notoriously difficult to work with as they degrade very rapidly. The cytoplasmic staining is a result of HIF1-alpha degradation. I hope that this helps. if your customer has any further questions they may find our guide to working with hypoxia antibodies to be useful.
  16. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  17. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Mouse Monoclonal
Species Human, Mouse, Rat...MORE
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     1 Review

22 Publications
NBP1-89843
Immunocytochemistry/Immunofluorescence: HYPB Antibody [NBP1-89843] - Staining of human cell line A-431 shows positivity in nucleus &amp; cytoplasm.Immunohistochemistry-Paraffin: HYPB Antibody [NBP1-89843] - Staining of human oral mucosa shows moderate nuclear positivity in squamous epithelial cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

NB100-124
Western Blot: HIF-1 beta Antibody (H1beta234) [NB100-124] - Analysis of HIF-1 beta in HeLa nuclear extract.Immunohistochemistry: HIF-1 beta Antibody (H1beta234) [NB100-124] - Immunohistochemical staining of human glioblastoma multi-forme utilizing anti-HIF-1 Beta.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, ChIP, GS...MORE

     3 Reviews

74 Publications
NBP1-84836
Western Blot: EPO Antibody [NBP1-84836] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11&lt;br/&gt;Lane 2: Human cell line RT-4&lt;br/&gt;Lane 3: Human cell line U-251MG sp&lt;br/&gt;Lane 4: Human plasma (IgG/HSA depleted)&lt;br/&gt;Lane 5: Human liver tissue&lt;br/&gt;Lane 6: Human tonsil tissueImmunohistochemistry-Paraffin: EPO Antibody [NBP1-84836] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NB100-485
Western Blot: Von Hippel Lindau Antibody [NB100-485] - Detection of VHL in human kidney samples (30 ug)using NB 100-485. ECL detection 10 seconds. Lane 1: 0.5 ug/ml primary Lane 2: 2 ug/ml primary

Rabbit Polyclonal
Species Human
Applications WB, IP

     1 Review

2 Publications
NB100-122
Western Blot: HIF-2 alpha Antibody [NB100-122] - Western blot of 786-O cells without or with VHL overexpression. Image from verified customer review.Immunocytochemistry/Immunofluorescence: HIF-2 alpha Antibody [NB100-122] - Detection of HIF-2 Alpha (Green) in RCC4 cells using NB100-122. Nuclei (Blue) are counterstained with Hoechst 33258.Immunohistochemistry: HIF-2 alpha Antibody [NB100-122] - Hif-2 alpha immunoreactivity in human cardiac myocytes.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, ChIP, ELISA...MORE

     18 Reviews

307 Publications
NB100-56749
Western Blot: AKT1/2 [p Ser473] Antibody (104A282) [NB100-56749] - Analysis of phospho AKT using phospho AKT antibody at 2 ug/ml against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test.

Mouse Monoclonal
Species Human, Mouse
Applications WB

     1 Review

6 Publications
NB110-39113
Western Blot: GLUT1 Antibody [NB110-39113] - GLUT 1 in A549 cells. Image from verified customer review.Immunocytochemistry/Immunofluorescence: GLUT1 Antibody [NB110-39113] - GLUT1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Western Blot: GLUT1 Antibody [NB110-39113] - Analysis of GLUT1 on A. mouse kidney membrane protein and B. rat kidney membrane protein.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ChIP, ICC/IF...MORE

     2 Reviews

7 Publications
NB100-417
Western Blot: Carbonic Anhydrase IX Antibody [NB100-417] - Carbonic Anhydrase IX on various human cell lysates. Image from verified customer review.Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX Antibody [NB100-417] - Immunofluorescence of human RCC tumor cryosections using NB100-417 (Panel A). Panel B shows staining with normal rabbit serum.Immunohistochemistry-Frozen: Carbonic Anhydrase IX Antibody [NB100-417] - Analysis of human colon carcinoma, xenografted in mice. Image courtesy of an anonymous product review.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, GS, ICC/IF...MORE

     3 Reviews

73 Publications
NB100-57386
Western Blot: p53 Antibody (H53C2) [NB100-57386] - p53 in carcinoma cell extract. Image from verified customer review.

Mouse Monoclonal
Species Human
Applications WB, IHC, IP

     1 Review

NBP1-19924
Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NBP1-19924] - extracts from Jurkat PMA cells .Immunohistochemistry-Paraffin: ERK1 [p Thr202, p Tyr204] Antibody [NBP1-19924] - Paraffin-embedded human breast carcinoma tissue.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

     1 Review

2 Publications
NBP1-47842
Western Blot: ERK2 Antibody (6E5) [NBP1-47842] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK2 monoclonal antibody.Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Staining of paraffin-embedded pancreas tissue using anti-ERK2 mouse monoclonal antibody.Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERK2.

Mouse Monoclonal
Species Human, Rat, Canine...MORE
Applications WB, IHC, IHC-P

NBP1-19855
Western Blot: mTOR Antibody [NBP1-19855] - extracts from NIH/3T3 cells treated with Wortmannin 40mM 24hours.Immunohistochemistry-Paraffin: mTOR Antibody [NBP1-19855] - Immunohistochemistry (IHC) analyzes of mTOR pAb in paraffin-embedded human brain tissue.Immunohistochemistry: mTOR Antibody [NBP1-19855] - Human brain tissue.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC...MORE

     1 Review

1 Publication
NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Expression of HIF-1 in intresititial pig cells under 12% of 02 condition. Image from verified customer review.Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, ChIP, ELISA...MORE

     21 Reviews

447 Publications
NB300-605
Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Image from verified customer review.Immunocytochemistry/Immunofluorescence: iNOS Antibody [NB300-605] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a iNOS polyclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Both normal and cancer biopsies of deparaffinized human Heart tissue.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, ICC/IF, IHC...MORE

     1 Review

11 Publications
NBP1-96554
Western Blot: TIMP1 Antibody (EPR1550) [NBP1-96554] TIMP-1 recombinant protein.Immunohistochemistry-Paraffin: TIMP1 Antibody (EPR1550) [NBP1-96554] - Analysis of paraffin-embedded human liver tissue using anti-TIMP-1.Western Blot: TIMP1 Antibody (EPR1550) [NBP1-96554] - (A) HL-60+TPA and (B) PBMC lysates.

Rabbit Monoclonal
Species Human, Rat, Mouse (Negative)
Applications WB, IHC, IHC-P

1 Publication
NBP1-19532
Immunocytochemistry/Immunofluorescence: TNF alpha Antibody [NBP1-19532] - TNF alpha antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Immunohistochemistry-Paraffin: TNF alpha Antibody [NBP1-19532] - Immunohistochemical staining of TNF alpha in mouse intestine.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC...MORE

     1 Review

4 Publications
NB100-137
Western Blot: PHD2/HIF Prolyl Hydroxylase 2 Antibody [NB100-137] -   Western Blot analysis of human PHD2, using NB100-137. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10ng/lane) and whole cell lysate from HeLa cells.Immunocytochemistry/Immunofluorescence: PHD2/HIF Prolyl Hydroxylase 2 Antibody [NB100-137] - Immunofluorescence of endogenous PHD2 in U2OS cells. Image from verified customer review.Immunohistochemistry: PHD2/HIF Prolyl Hydroxylase 2 Antibody [NB100-137] - Staining of  Vascular Endothelium 40X in Lung using NB100-137.

Rabbit Polyclonal
Species Human, Mouse (Negative), Rat (Negative)
Applications WB, FLOW, ICC/IF...MORE

     5 Reviews

42 Publications

Bioinformatics Tool for HIF-1 alpha

Discover related pathways, diseases and genes to HIF-1 alpha. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Blogs on HIF-1 alpha

Check out the latest blog posts on HIF-1 alpha.
HIF-1 beta: A dimerization partner of HIF-1 alpha required for an adaptive response to hypoxia
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia-indu...  Read full blog post.

HIF-1 Alpha: Infographic
Encoded by the HIF1A gene, HIF-1 alpha has a critical role in cellular response to hypoxia. In hypoxic conditions, HIF-1 alpha activates the transcription of several genes to facilitate metabolic reaction for lack of oxygen. In normoxic conditions, HI...  Read full blog post.

Controlling the HIF-1 Switch
Hypoxia-inducible factor-1 is a major transcription factor composed of two subunits: HIF-1alpha and HIF-1 beta. Under normoxic conditions, HIF-1 alpha is targeted to proteosomal degradation via ubiquitination. On the other hand during hypoxic conditio...  Read full blog post.

Hypoxia Inducible Factor-1 beta and Cancer Development
Hypoxia inducible factor-1 (HIF-1) is a major transcription factor that is composed of two subunits: HIF-1 alpha and HIF-1 beta, the latter being a constitutively-expressed aryl hydrocarbon receptor nuclear transporter (ARNT). Under normoxic condition...  Read full blog post.

HIF Prolyl Hydroxylase 2: an important Oxygen Sensor Protein
Prolyl hydroxylase domain (PHD) proteins, including PHD1, PHD2, and PHD3, mediate oxygen-dependent degradation of hypoxia-inducible factor (HIF) alpha subunits. Suppression of PHD enzymes leads to stabilization of HIFs and offers a potential treatment...  Read full blog post.

Myc-tag: The "Monkey Wrench" of Proteomic Tools
c-Myc is a well-characterized transcription factor encoded by the c-Myc gene on human chromosome 8q24. This cellular proto-oncogene, also known as p62, is commonly activated in a variety of tumor cells and plays a crucial role in cellular proliferatio...  Read full blog post.

MAT2a, MAT2b, HIF-1 alpha: Roles in Liver Cancer and DNA methylation
Methionine Adenosyltransferase II alpha, also known as MAT2a, is a catalytic subunit of methionine adenosyltransferase (MAT) and essential enzyme for the catalysis of the principle biological methyl donor, S-adenosylmethionine (SAM) from methionine an...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

Carbonic Anhydrase IX and Hypoxic Response: A Path to Tumors
Carbonic Anhydrase IX (CA-IX) is an enzyme that is induced under hypoxic conditions. This enzyme is rarely present in normal cells and is responsible for controlling tumor pH. CA-IX is a transmembrane glycoprotein of the zinc metalloenzyme family. Thi...  Read full blog post.

HIF-2 alpha Antibody Throws New Light on EPO Dilemma
The HIF family are heterodimeric, oxygen-sensitive transcription factors comprising an alpha and beta subunit which are normally dissociated in normoxic conditions. Our antibody catalog contains products targeting all the Hypoxia Inducible Factor isof...  Read full blog post.

HIF Antibodies: Beyond HIF-1 alpha
The hypoxia inducible factors are a family of heterodimeric transcription factors which are activated in response to lowered oxygen levels, or hypoxia. Although it may seem that HIF-1 alpha receives all the attention, other HIF antibodies, such as the...  Read full blog post.

A Role for HIF-1 alpha Antibody in Renal Research
The Hypoxia Inducible Factors (HIFs) are a family of mammalian transcription factors which are expressed in response to low cellular oxygen concentrations (hypoxia). Three human hypoxia inducible factors have been identified, HIF-1, HIF-2 and HIF-3, e...  Read full blog post.

Hypoxia-Inducible Factors and Homeostasis
Hypoxia-Inducible Factor-1 (HIF-1) is a highly conserved heterodimeric transcription factor. Novus' antibody catalogue contains an extensive range of both HIF-1 alpha and HIF-1 beta, useful for hypoxia, angiogenesis, cancer and many other areas of res...  Read full blog post.

We Heart HIF: HIF-1 Improves Angiogenesis Therapy in Ischemic Heart Tissue
Ischemic cardiovascular disease is the leading cause of death for both men and women in the US and most other industrialized countries. Most commonly caused by atherosclerosis of the coronary arteries, ischemic cardiovascular disease is characterized ...  Read full blog post.

Using the Hif-1 Alpha Antibody in Prostate Cancer Research
The Hypoxia-inducible Factor 1 (HIF1) protein is a heterodimeric transcription factor which plays an important role in mammalian oxygen homeostasis in conditions of hypoxia, or low oxygen concentration. HIF-1 alpha antibody reagents are widely used in...  Read full blog post.

HIF-1 Beta Antibody Studies and Developments in Hypoxia Research
We at Novus Biologicals have a large HIF-1 (Hypoxia Inducible Factor-1) antibody catalog, with twenty HIF-1 beta antibody products specifically targeted to the beta subunit of HIF-1, a heterodimer which is known to contribute to many human diseases ar...  Read full blog post.

The Role of LC3 within the Autophagic Pathway
We at Novus Biologicals have a broad antibody databasecovering the area of autophagy - over 1400 reagents in total. Autophagy is bulk degradation of cytoplasmic components - literally, self-digestion of the cell. Double-membrane vesicles, called autop...  Read full blog post.

The Link Between Base Excision Repair and Cancer Antibodies
Base excision repair (BER) is the most fundamental DNA repair mechanism, dealing with alterations arising in individual DNA bases during cellular metabolism. We at Novus Biologicals have a large BER antibody database, which has proven important in var...  Read full blog post.