Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide: C-NEFYDGDHDNDKESDVEI conjugated to KLH, corresponding to amino acids 411-428 of human and chicken HDAC3.
Localization:
Nuclear
Epitope:
The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
Species Reactivity:
Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.
Applications:
Uses:
This antibody is useful for immunoprecipitation and Western Blot. ChIP: Use 2ul for 106 cells. IHC-P: Use human lymph node sections. WB: Use nuclear HeLa human epitheloid carcinoma cells. Predicted molecular weight: 48.8 kDa.
Dilutions:
chromatin immunoprecipitation 2 ul for10E6 cells,
immunoprecipitation 1/2500,
Western Blot ,
Immunohistochemistry-Paraffin 1/500
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
0.01M PBS [pH7.4]
Preservative:
15mM Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.