HDAC3 Antibody (NB500-126)

HDAC3 Antibody

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HDAC3 Antibody Summary:
Species:Hu, Mu, Rt
Applications:ChIP, IHC-P, IP, WB
Clonality:Polyclonal
Gene:HDAC3
Purity:IgG purified
Host:Rabbit
Specificity:Anti-Histone Deacetylase 3 specifically recognizes histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kD).
Publications:Antibody has been mentioned in at least 6 Publications.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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HDAC3 Antibody Details:
Isotype:IgG
Immunogen:Synthetic peptide: C-NEFYDGDHDNDKESDVEI conjugated to KLH, corresponding to amino acids 411-428 of human and chicken HDAC3.
Localization:Nuclear
Epitope:The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
Species Reactivity:

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Applications:
Uses:This antibody is useful for immunoprecipitation and Western Blot. ChIP: Use 2ul for 106 cells. IHC-P: Use human lymph node sections. WB: Use nuclear HeLa human epitheloid carcinoma cells. Predicted molecular weight: 48.8 kDa.
Dilutions:chromatin immunoprecipitation 2 ul for10E6 cells, immunoprecipitation 1/2500, Western Blot , Immunohistochemistry-Paraffin 1/500
Unit Size:0.05 ml
Concentration:13.3 mg/ml
Positive Controls:4 Positive Controls
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:0.01M PBS [pH7.4]
Preservative:15mM Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: HDAC3
Entrez8841 (Human)
15183 (Mouse)
84578 (Rat)
 
Background:

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

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Working with HDAC3

HDAC3 Antibody Images (1)
Western blot: HDAC3 Antibody [NB500-126]-Monoclonal Anti-HDAC3 antibody produced in mouse clone 3E11, purified immunoglobulin, buffered aqueous solution
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Publications (6)

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Jump to: Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Positive Controls
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
NIH 3T3 Whole Cell LysateNB800-PC8(2)

Hela Whole Cell LysateNB800-PC1WB(2)

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Other Positive Controls
  • HeLa, NIH3T3, and human lymph node
Antibody Pairs (1)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
HDAC3 Antibody PairH00008841-PW1HuIP, WB

Lysates (3)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
HDAC3 LysateH00008841-T01HuWB(2)

HDAC3 LysateNBL1-11481WB(1)

HDAC3 LysateH00008841-T02WB

Peptides and Proteins
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
HDAC3 Partial Recombinant ProteinH00008841-Q01HuELISA, WB(1)

HDAC3 Recombinant ProteinH00008841-P01HuELISA, WB(1)

Primary Antibodies (20)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
HDAC3 Antibody (Y415)NB110-57033Hu, Mu, RtICC, IHC-P, IP, WB(3)(2)

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HDAC3 AntibodyNBP1-67592Hu, Mu, RtELISA, IF, IHC, WB(3)

HDAC3 AntibodyNBP1-61624Hu, Mu, RtELISA, IF, IHC, WB(3)

HDAC3 Antibody (2A3)H00008841-M03HuELISA, IF, WB(5)

... and 15 more. See All »
RNAi (1)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
HDAC3 RNAiH00008841-R01Hu(1)(4)

Secondary Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Rabbit IgG Antibody [HRP]NB730-HRbELISA, ICC, IHC-P, WB(5)

Rabbit IgG Antibody [FITC]NB730-FRbICC, IHC-P(1)

Rabbit IgG Antibody [Biotin]NB730-BRbELISA, ICC, IHC-P, WB

Rabbit IgG, H/L Chains Antibody [DyLight 488]NBP1-72944RbFLISA, IF, WB(3)

Rabbit IgG Antibody [Alkaline Phosphatase]NB730-APRbELISA, ICC, IHC-P, WB(1)

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HDAC3 in Context


Research Areas related to HDAC3 (6):
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