HDAC3 Antibody (NB100-79798) | Novus Biologicals

HDAC3 Antibody (NB100-79798) (0.1 ml)

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	Catalog Number	NB100-79798
	Size(s)	0.1 ml	Price:
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anti-EC 3.5.1.98 antibody, anti-SMAP45 antibody, anti-HD3RPD3-2RPD3 antibody, anti-histone deacetylase 3 antibody

HDAC3 Antibody Summary:

Species:	Hu, Mu	Gene:	HDAC3
Applications:	WB, IHC-P, IP	Purity:	Immunogen affinity purified
Clonality:	Polyclonal	Host:	Rabbit
Publications:

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RNAi
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HDAC3 Antibody Details:

Isotype:	IgG
Immunogen:	The epitope recognized by this antibody maps to a region between residue 375 and the C-terminus (residue 428) of human Histone Deacetylase 3 using the numbering given in Swiss-Prot entry O15379 (GeneID 8841).

Species Reactivity:

Human and Mouse.

Applications:

Uses:	Western blot, Immunohistochemistry-Paraffin and Immunoprecipitation
Dilutions:	Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot 1:5000-1:15000 immunoprecipitation 2-5 mcg/mg lysate
Unit Size:	0.1 ml
Concentration:	0.2 mg/ml
Positive Controls:	1 Positive Controls

Notes:

Western Blot Image 1 Notes: Detection of Human and Mouse HDAC3 by Western Blot and Human HDAC3 by Immunoprecipitation. Samples: A) Whole cell lysate (WCL) from HeLa (5, 15 and 50 mcg), Ramos (R; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. B) WCL (1 mg for IP; 1/4 of IP reaction/gel) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC3 Antibody BL2127 (Cat. No. NB100-79798) used at 0.02 mcg/ml for WB (A) and at 3 mcg/mg lysate for IP (B). Immunoprecipitation reactions were also performed with another anti-HDAC3 antibody BL2125 (to an upstream epitope) and BL2128 (to a downstream epitope) and normal rabbit IgG.

Packaging:

Storage:	Store at 4°C. Do not freeze.
Buffer:	Tris-buffered Saline containing 0.1% BSA
Preservative:	0.09% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: HDAC3

Entrez:	8841 (Human)
Entrez:	15183 (Mouse)
Uniprot:	O15379 (Human)

Related Genes:

Background:

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Images (2)


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HDAC3 Antibody Images (2)

	Western Blot: HDAC3 Antibody [NB100-79798] - Detection of Human and Mouse HDAC3 by Western Blot and Human HDAC3 by Immunoprecipitation. Samples: A) Whole cell lysate (WCL) from HeLa (5, 15 and 50 mcg), Ramos (R; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. B) WCL (1 mg for IP; 1/4 of IP reaction/gel) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC3 Antibody BL2127 (Cat. No. NB100-79798) used at 0.02 mcg/ml for WB (A) and at 3 mcg/mg lysate for IP (B). Immunoprecipitation reactions were also performed with another anti-HDAC3 antibody BL2125 (to an upstream epitope) and BL2128 (to a downstream epitope) and normal rabbit IgG.

	Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-79798] - FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-HDAC3 at a dilution of 1:250. DAB staining using Immunohistochemistry Accessory Kit