Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
A synthetic peptide corresponding to residues in the C-term of human Histone deacetylase 3 was used as immunogen.
Species Reactivity:
Reacts in Human, Mouse, Rat.
Applications:
Uses:
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 49kDa.
Dilutions:
Western Blot 1:5000-10000, Immunohistochemistry-Paraffin 1:250-500, Immunocytochemistry 1:250-500, Immunoprecipitation 1:50
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA
Preservative:
0.01% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Histone deacetylase 3 belongs to the histone deacetylase family type 1 which actively deacetylate Histones. Currently 3 known isoforms exists (HDAC3, HDAC3A and HDAC3C) (1). HDAC3 represses transcription and binds transcription factor YY1 to form a large complex. N-Cor binds HDAC3 in a different manner than other HDACs. In addition to N-Cor, HDAC3 will form a complex with corepressor SMRT (2). HDAC3 is also known to interact with DAXX, HDAC10 and DACH1.
Immunohistochemistry: Histone Deacetylase 3 (C-term) Antibody (Y415) [NB110-57033] - Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-Histone deacetylase 3 (C-term) (cat. # 110-57033).
Western Blot: Histone Deacetylase 3 (C-term) Antibody (Y415) [NB110-57033] - Western blot analysis on K562 cell lysate using anti-Histone Deacetylase 3 (C-term) (cat. #110-57033), 1:10,000
Immunofluorescent: Histone Deacetylase 3 (C-term) Antibody (Y415) [NB110-57033] - Immunofluorescent staining of HeLa cells using anti-Histone deacetylase 3 (C-term) (cat. # 110-57033).