Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Chickens were immunized with HA cleavage site (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Chicken anti- human HA affinity purified antibodies were coupled to peptide immobilized on solid support.
Species Reactivity:
Epitope Tag. Non-species specific.
Applications:
Uses:
Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 Cytochemistry 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot, and immunocytochemistry.
Dilutions:
ELISA, Immunocytochemistry, immunofluorescence, immunoprecipitation, Western Blot
Unit Size:
0.1 mg
Concentration:
Please see the vial label for concentration.
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
Phosphate Buffered Saline, pH 7.2
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Background:
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA
molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Bedard, J. E. J., Purnell, J. D., Ware, S. M. (2007) Nuclear import export signals are essential for proper cellular trafficking function of ZIC3, 16, 187-198.
2. Wang, J., Shao, Y., Bennett, T. A., Shankar, R. A., Wightman, P. D., Reddy, L. G. (2006) The Functional Effects of Physical Interactions among Toll-like Receptors 7, 8, 9, 281, 37427-37434.