H2AX Antibody (NB100-638)
H2AX Antibody
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Distributor Data...
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Anti-Histone H2AX antibody, anti-Histone H2AFX antibody, anti-Histone H2A/X antibody, anti-Histone H2A.x antibody, |
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H2AX Antibody Summary: | | Gene: | H2AFX |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | | | Publications: | Antibody has been mentioned in at least 1 Publication. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional H2AX Products H2AX Antibody Details: | Isotype: | IgG |
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| Immunogen: | The epitope recognized by this antibody maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014). |
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| Marker: | DNA double strand break Marker |
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| Uses: | This antibody can be used for Western blot and immunofluorescence. Immunoprecipitation: not tested (please try NB 100-383) |
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| Dilutions: | Immunocytochemistry 1:400-1:800, Immunofluorescence, Western Blot 1:5000-1:25000 |
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| TMW: | 15 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | 1.0 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Tris-citrate/phosphate buffer, pH 7 to 8 |
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| Preservative: | 0.1% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.
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Images (2) Reviews (0) Related Products (12) Exploring H2AX Working with H2AX
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Jump to: Publications, Images, Ask a Question
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Working with H2AX
H2AX Antibody Images (2) Immunofluorescent: H2AX Antibody [NB100-638] - Detection of mouse H2AX by immunofluorescence in asynchronous wildtype (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts using NB100-638 at 2 ug/ml. Detection: Rhodamine labeled goat anti-Rabbit IgG. |  Detection of human H2AX in 293T nuclear lysates and wildtype (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts using NB 100-638. 10 second ECL exposure. |
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Publications (1)
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Jump to: Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive ControlsPeptides and ProteinsPrimary Antibodies (8)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| H2AX Antibody | NB100-383 | Hu, Mu | IP, WB | (1) | (3) | | | | H2AX Antibody | NBP1-61896 | Hu, Mu, Rt | ELISA, IHC, WB | (2) | | | | | H2AX Antibody | NB100-78355 | Hu | IF, WB | (2) | (4) | | | | H2AX [p Ser139] Antibody | NBP1-64745 | Hu, Mu, Rt | ELISA, WB | | | | | | H2AX Antibody | H00003014-D01P | Hu | WB | | | | | | ... and 3 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | Rabbit IgG Antibody [Cy3] | NB120-6939 | Rb | ELISA, FACS, ICC, IF, IHC-Fr, IHC-P | | (1) | | | | See All » |
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