GTPase Colorimetric Assay Kit 5 x 480 Tests

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Colorimetric GTPase Assay Kit 5Sets [602-0122] - Absorbance versus amount of enzyme
Colorimetric GTPase Assay Kit 5Sets [602-0122] - Principle of the GTPase assay kit
Colorimetric GTPase Assay Kit 5Sets [602-0122] - Standard curve for P
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Ordering Information: 602-0122

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  • Size(s)
    5 x 480 Tests
  • Availability
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GTPase Colorimetric Assay Kit Summary

Colorimetric assays for GTPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate.

At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes. Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance.

Uniquely, Innova Biosciences GTPase assay kits contain highly purified GTP, which have had any free inorganic phosphate removed - this means that assays have the lowest possible background signal.

PiColorLock Phosphate Detection Systems can also be purchased for developing simple assays for any phosphate-generating enzyme.

This non radioactive colorimetric assay kit uses a 96 well format, and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening.
Kit Type
Colorimetric Assay Kit
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602-0122 in the following applications:

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    5 x 480 Tests
    Concentration is not relevant for this product. Please see the protocols for proper use of this product.
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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    Publications for GTPase Kit (602-0122) (23)

    Read what people are writing who have used GTPase Kit (602-0122).

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    Publication Applications Species
    Garcia-Regalado A, Guzman-Hernez ML, Ramirez-Rangel I et al. G Protein-coupled Receptor-promoted Trafficking of G{beta}1{gamma}2 Leads to AKT Activation at Endosomes via a Mechanism Mediated by G{beta}1{gamma}2-Rab11a Interaction. Mol Biol Cell;19(10):4188-4200. 2008 [PMID: 18701709]
    Villarroya M, Prado S, Esteve JM et al. Characterization of Human GTPBP3, a GTP-Binding Protein Involved in Mitochondrial tRNA Modification. Molecular and Cellular Biology, 28 (24) pp 7514-7531. 2008 [PMID: 18852288]
    Moertel L, Gobert GN, McManus DP. Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum. Parasitology 2008 [PMID: 18294425]
    Marino SF. High-level production and characterization of a G-protein coupled receptor signaling complex. FEBS J. 2009 [PMID: 19645726]
    Tu C, Zhou X, Tropea JE et al. Structure of ERA in complex with the 3' end of 16S rRNA: Implications for ribosome biogenesis. PNAS 2009 [PMID: 19706445]
    Richter K, Brar S, Ray M et al. Speckled-like Pattern in the Germinal Center (SLIP-GC), a Nuclear GTPase Expressed in Activation-induced Deaminase-expressing Lymphomas and Germinal Center B Cells. JBC 2009 [PMID: 19734146]
    Wan T, Liu T, Zhang H et al. AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling. JBC 2010 [PMID: 19948740]
    Wang Y, Liang D, Wang S et al. Role of the G-protein and tyrosine kinase-Rho/ROK pathways in 15-hydroxyeicosatetraenoic acid induced pulmonary vasoconstriction in hypoxic rats. J Biochem 2010 [PMID: 20139061]
    Xiong Y, Coombes CE, Kilaru A et al. GTPase activity plays a key role in the pathobiology of LRRK2. PLoS Genet. 2010 [PMID: 20386743]
    Pungaliya PP, Bai Y, Lipinski K et al. Identification and Characterization of a Leucine-Rich Repeat Kinase 2 (LRRK2) Consensus Phosphorylation Motif. PLoS One 2010 [PMID: 21060682]
    Wang Z, Jiang H, Chen S et al. The Mitochondrial Phosphatase PGAM5 Functions at the Convergence Point of Multiple Necrotic Death Pathways. Cell 2012 [PMID: 22265414]
    Stafa K, Trancikova A, Webber PJ et al. GTPase Activity and Neuronal Toxicity of Parkinson's Disease-Associated LRRK2 Is Regulated by ArfGAP1. PLoS Genet. 2012 [PMID: 22363216]
    Xiong Y, Yuan C, Chen R et al. ArfGAP1 Is a GTPase Activating Protein for LRRK2: Reciprocal Regulation of ArfGAP1 by LRRK2. J Neurosci 2012 [PMID: 22423108]
    Han JM, Jeong SJ, Park MC et al. Leucyl-tRNA Synthetase Is an Intracellular Leucine Sensor for the mTORC1-Signaling Pathway. Cell 2012 [PMID: 22424946]
    Gopalakrishnan J, Chim YC, Ha A et al. Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment. Nat Cell Biol 2012 [PMID: 22729084]
    Gonzalez-Flores JN, Gupta N, DeMong LW, Copeland PR. The selenocysteine-specific elongation factor contains a novel and multi-functional domain. J Biol Chem. 2012 [PMID: 22992746]
    Mallozzi C, D'Amore C, Camerini S et al. Phosphorylation and nitration of tyrosine residues affect functional properties of Synaptophysin and Dynamin I, two proteins involved in exo-endocytosis of synaptic vesicles. Biochim Biophys Acta. 2012 [PMID: 23103755]
    Zhang K, Fishel Ben Kenan R, Osakada Y et al. Defective Axonal Transport of Rab7 GTPase Results in Dysregulated Trophic Signaling. J Neurosci 2013 [PMID: 23616551]
    Alaganan A, Fentress SJ, Tang K et al. Toxoplasma GRA7 effector increases turnover of immunity-related GTPases and contributes to acute virulence in the mouse. PNAS 2013 [PMID: 24390541]
    Santhosh KT, Sikarwar AS, Hinton M et al. Thromboxane Receptor Hyper-Responsiveness in Hypoxic Pulmonary Hypertension Requires Serine 324. Br J Pharmacol. 2013 [PMID: 24490858]
    Gundner AL, Hahn I, Sendscheid O et al. The PIKE Homolog Centaurin gamma Regulates Developmental Timing in Drosophila. PLoS One 2014 [PMID: 24845618]
    Ellinger D, Glockner A, Koch J et al. Interaction of the Arabidopsis GTPase RabA4c with Its Effector PMR4 Results in Complete Penetration Resistance to Powdery Mildew. Plant Cell 2014 [PMID: 25056861]
    Yan J, Liu XH, Han MZ et al. Blockage of GSK3 beta-mediated Drp1 phosphorylation provides neuroprotection in neuronal and mouse models of Alzheimer’s disease. Neurobiol Aging 2014 [PMID: 25192600]

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    FAQs for GTPase (6)

    1. May we have the protocol for 602-0120?The info. from seems insufficient.
      • Here is the corresponding full protocol for your customer for this kit. I hope they find this information useful.
    2. I have phosphate in my enzyme. What can I do?
      • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
    3. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
      • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
    4. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
      • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
    5. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
      • Yes, only the phosphate will give a signal; pyrophosphate will not.
    6. I was wondering if any of the Lightning-Link kit components contain phosphates, and whether you can tell me the concentration?
      • The Lightning-Link kits do contain phosphate. The final concentration will vary depending on the amount of antibody you add to the vial, but should always be less than 15mM.

    Additional GTPase Colorimetric Assay Kit Products

    GTPase 602-0122

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    OPA1 belongs to the Dynamin large GTPase protein family. OPA1 exists as a single-pass membrane protein localized in the mitochondrial inner membrane and also as a soluble form in the mitochondrial intermembrane space. There, it is a key player in fusi...  Read full blog post.