GFP Antibody (NB100-1678)
GFP Antibody [Biotin]
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Distributor Data...
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anti-Green Fluorescent Protein antibody, |
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GFP Antibody [Biotin] Summary: | | Gene: | GFP |
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| Purity: | IgG purified |
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| Host: | Goat |
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| | Specificity: | Antibody recognizes wild type, recombinant and enhanced forms of GFP (EGFP). |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional GFP Products GFP Antibody [Biotin] Details: | Conjugate: | Biotin |
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| Isotype: | IgG |
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| Immunogen: | The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish (Aequorea victoria). |
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| Uses: | *Optimal dilutions should be determinedby the end user. |
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| Dilutions: | Immunohistochemistry-Paraffin 1/500 - 1/3000,
Western Blot 1/1000 - 1/5000 |
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| Unit Size: | 0.2 mg |
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| Concentration: | 1 mg/ml |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Buffer: | BSA and 0.02M Potassium Phosphate, 0.15 Sodium chloide pH 7.2 |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
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Reviews (2) Related Products (60) Exploring GFP Working with GFP
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Jump to: Reviews, Ask a Question
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Working with GFP
GFP Antibody [Biotin] Images (0)
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Reviewed by:
Laura Shankman 11/8/2011
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Application:
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IF
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Sample Tested:
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Mouse Aorta, Liver, Brain, Lung
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Species:
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Mouse
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Sample Preparation:
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4% PFA (30min)
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Sample Pretreatment:
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5mL 4% PFA perfusion
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Lot:
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23463
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Results:
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Very clean staining using a SA-488 or TSA-488 secondary
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Blocking Buffer:
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10% Goat Serum in PBS+FSG
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Blocking Time:
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1 hr (30min + SA, 30min + biotin)
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Blocking Temperature:
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RT Fahrenheit
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Dilution Ratio:
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1:100
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Incubation Time:
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Overnight
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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PBS+FSG 10% goat serum
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Description:
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Streptavidin 488
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Manufacturer Catalog #:
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S11223
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Dilution Ratio:
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1:100
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Visualization Method:
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Confocal Microscope
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Did you use controls?:
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Yes
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Positive Control:
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eYFP transgenic mouse
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Negative Control:
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eYFP- transgenic mouse and goat IgG-biotinylated
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Reviewed by:
Anonymous 2/17/2010
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Jump to: Peptides and Proteins, Primary Antibodies, Secondary Antibodies
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Related ProductsPeptides and ProteinsPrimary Antibodies (34)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| GFP Antibody | NB600-308 | All | ELISA, IHC-P, WB | (1) | (16) | | | | GFP Antibody | NB600-303 | All | ELISA, IHC-P, WB | (1) | (12) | | | | GFP Antibody | NB600-310 | All | ELISA, ICC, IF, WB | (2) | | | | | GFP Antibody | NB100-1770 | All | ELISA, IF, IHC, IHC-P, WB | (3) | (4) | | | | GFP Antibody (9F9.F9) | NB600-597 | All | ELISA, ICC, WB | (2) | (1) | | | | ... and 29 more. See All » |
Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Goat IgG Antibody [FITC] | NB710-F | Gt | ICC, IHC-P | | | | | | Goat IgG Antibody [Biotin] | NB710-B | Gt | ELISA, ICC, IHC-P, WB | | | | | | Goat IgG Antibody [Alkaline Phosphatase] | NB710-AP | Gt | ELISA, ICC, IHC-P, WB | | | | | | Goat IgG Antibody [HRP] | NB710-H | Gt | ELISA, ICC, IHC-P, WB | | (4) | | | | Goat IgG Antibody [Cy3] | NB120-6949 | Gt | ELISA, FACS, ICC, IF, IHC-Fr, IHC-P | | | | | | See All » |
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Jump to: Research Areas
| GFP in Context
Research Areas related to GFP (1): |
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