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![Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Western blot of GFP recombinant protein detected with monoclonal anti-GFP antibody (NB600-597). GFP recombinant protein was expressed in HeLa cells, where 50 ng (Lanes 1), 100 ng (Lanes 2) and 500 ng (Lanes 3) of lysate were loaded per lane. NB600-597 detects a 27 kDa band corresponding to the epitope tag GFP. The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl. The membrane was probed with the primary antibody diluted to 1.0 ug/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDye800 conjugated Goat-a-Mouse IgG [H&L].](http://images.novusbio.com/images/GFP-Antibody-(9F9.F9)-Western-Blot-NB600-597-img0004.jpg "Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Western blot of GFP recombinant protein detected with monoclonal anti-GFP antibody (NB600-597). GFP recombinant protein was expressed in HeLa cells, where 50 ng (Lanes 1), 100 ng (Lanes 2) and 500 ng (Lanes 3) of lysate were loaded per lane. NB600-597 detects a 27 kDa band corresponding to the epitope tag GFP. The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl. The membrane was probed with the primary antibody diluted to 1.0 ug/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDye800 conjugated Goat-a-Mouse IgG [H&L].")
![Western Blot: GFP Antibody (9F9.F9) [NB600-597] Known amounts of recombinant GFP protein were spiked into a HeLa cell-derived lysates and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS supplemented with 1% BSA (for 1 h at 4°C prior to probing the blot with the anti-GFP monoclonal antibody diluted 1:1,000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG was performed at a dilution of 1:20,000 for 1h at 4°C. Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection](http://images.novusbio.com/images/GFP Antibody (9F9.F9)-Western Blot-NB600-597-img0002.jpg "Western Blot: GFP Antibody (9F9.F9) [NB600-597] Known amounts of recombinant GFP protein were spiked into a HeLa cell-derived lysates and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS supplemented with 1% BSA (for 1 h at 4°C prior to probing the blot with the anti-GFP monoclonal antibody diluted 1:1,000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG was performed at a dilution of 1:20,000 for 1h at 4°C. Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection")
