Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
A synthetic peptide (CVESAKIRAKYP) corresponding to the N-terminal of human GABARAP L2 (GABARAPL2) protein has been used as the immunogen. The sequence is homologous with mouse and rat form of GABARAP L2 (GABARAPL2).
IHC-Fr, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. The optimal dilution should be determined by the end user.
Dilutions:
Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Unit Size:
0.1 ml
Concentration:
Lyoph
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Lyophilized. Add diH20 to desired concentration.
Preservative:
No Preservative
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
FUNCTION: Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. SUBUNIT: Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. SUBCELLULAR LOCATION: Golgi apparatus. TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. SIMILARITY: Belongs to the MAP1 LC3 family.
This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.
Immunofluorescence: GABARAP L2 Antibody [R-142-100] - Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for GABARAP L2 appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
C is the merged picture of Figures A & B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for GABARAP L2 (GABARAPL2) appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were counter stained with Hoechst Dye (blue colour).
Immunofluorescence: GABARAP L2 Antibody [R-142-100] - B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were stained for GABARAP L2 (GABARAPL2) appearing in red counter-stained with Hoechst DyeHere. The merged picture is presented.
Immunofluorescent: GABARAP L2 Antibody [R-142-100] - A. Confocal microscopy on immunofluorescently detected MPO (in green) in cytospin-isolated human white blood cells counter-stained with Hoechst Dye. Here, the merged picture is presented.
Western Blot: GABARAP L2 Antibody [R-142-100] - Western blot under reduceding condition on Odora (an Olfactory cell line) cell lysate using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1:100.
Western Blot: GABARAP L2 Antibody [R-142-100] - Western blot under reduceding condition on 293 cell lysate using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1:100.
Okazaki N. et al. Brain Res. Mol. Brain Res. 85:1-12(2000) ;
2. Xin Y. et al. Genomics 74:408-413(2001) ;
3. The MGC Project Team. Genome Res. 14:2121-2127(2004)