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GABA Antibody

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Product Details

Summary
Product Discontinued
View other related GABA Primary Antibodies

Order Details


    • Catalog Number
      NBP1-78347
    • Availability
      Product Discontinued

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GABA Antibody Summary

Immunogen
GABA coupled to bovine serum albumin (BSA) with glutaraldehyde.
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
The antibody produces significant labeling at dilutions of 1/120 - 1/150 (which is equivalent to 1/12,000 - 1/15,000 using biotin-avidin/HRP technique in rat cerebellum and thalamus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Immunolabeling is completely abolished by preabsorption with excess GABA/BSA conjugate.

Reactivity Notes

Reacts with Rat, Mouse, Turtle, Frog

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Please contact technical support for detailed reconstituion instruction.

Notes

Fixative: 4% paraformaldehyde/0.3% glutaraldehyde in 0.1M phosphate buffer, pH 7.4;500 mL over ~ 20-30 min.Post Fixation: 1.5 hr. at 4C in 4% paraformaldehyde/ 0.3% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4. Note: Glutaraldehyde is a necessary component of fixation for the GABA antiserum. Higher levels of glutaraldehyde may be used if needed.

Alternate Names for GABA Antibody

  • y-Aminobutyric acid

Background

Without colchine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA Antibody (NBP1-78347) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for GABA Antibody (NBP1-78347) (0)

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Secondary Antibodies

 

Isotype Controls

Additional GABA Products

Array NBP1-78347

Blogs on GABA.

Brain size matters: MTOR regulates autophagy and number of cortical interneurons
By Jamshed Arslan Pharm.D. Interneurons transmit impulses between other neurons, in part, to facilitate the birth of neurons. Cortical interneurons themselves arise from the progenitors in the ventral telencephalo...  Read full blog post.

Using RPE65 as a tool to investigate ocular gene therapies
While not life threatening, blindness and retinal disease are profoundly debilitating and greatly affect quality of life.  Understandably, gene therapy has been subject to controversy given it’s potential effects on the rest of our cellular ...  Read full blog post.

New MECP2 Study Suggests Important Link to GABA
MECP2 antibodies are used in DNA methylation studies as well as research into Rett syndrome, a progressive neurological disorder caused by a mutation in the MECP2 gene. Now, a new study has been published by the laboratory where the Rett Syndrome gene...  Read full blog post.

PMP22 Antibodies Assist in PNS Myelin Repair Research
Novus Biologicals recently added a new  PMP22 antibody, conjugated to Biotin, to our antibody database. PMP22 (Peripheral Myelin Protein 22) is important to the structure of the myelin sheath in peripheral nerves, and is encoded by the PMP22 gene.Th...  Read full blog post.

A Shocking Truth: GABA Antibodies Developing New Therapies for Clinical Depression
Supplying antibodies for neuroscience research forms an important part of our work here at Novus Biologicals. Our antibody database is represented by more than 5,600 neuroscience products covering conditions ranging from sensory systems to neurodegene...  Read full blog post.

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