Fluoro-Trap Fluorescein Labeling Kit [FITC] 3 x 100ug reactions Summary:
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| Specificity: | The Fluoro-Trap Fluorescein labeling kit can be used to set up labeling reactions in seconds, simply by adding your protein to a film of reactive dye. Once the conjugate has formed, Fluoro-Trap resin is used to remove excess free label from the conjugate in one easy step. Fluoro-Trap labeling systems can be used to label antibodies and other proteins with high recoveries and minimal sample dilution.
Fluoro-Trap fluorescein labeling kits are currently available in a size for 3 x 100.
If your antibody needs to be concentrated, purified, or cleaned up, please look at our antibody clean up kits. |
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| Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
Fluoro-Trap Fluorescein Labeling Kit [FITC] 3 x 100ug reactions Details:
| Unit Size: | 3 x 100 ug reactions |
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| Concentration: | Concentration is not relevant for this product. Please see the protocols for proper use of this product. |
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Notes:
1. Storage and components
The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at 4C upon receipt.
Kit contents:
Three tubes of Fluorescein label
Three tubes of Fluoro-Trap resin
1 tube of Fluoro-Trap reaction buffer.
1 tube of Fluoro-Trap quencher
1 tube of Fluoro-Trap neutralizer
1 protocol
Frequently asked questions
Q1. What functional groups do I need on my protein?
Fluoro-Trap(TM) systems requires amine groups on the biomolecule to be labeled. All antibodies have multiple amine functions.
Q2. Do I have to label 100mg of antibody?
No. The amount does not have to be exactly 100mg but this quantity gives a molar ratio of antibody to dye that leads to good labeling for most antibodies. Varying the amount of antibody using a fixed amount of dye (as provided in the kit) can be used to generate conjugates with a varying average number of dye molecules per antibody. Experimentation in the range 50-200mg antibody per tube may be useful in fine tuning the performance of conjugates for specific applications.
Packaging:
| Storage: | The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at -20 °C upon receipt. |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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