Fibrillarin Antibody (38F3) 0.25 ml Nucleolar Marker

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Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Western blot analysis of Fibrillarin expression on yeast protein extracts using NB300-269.
Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - IF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry-Paraffin: Fibrillarin Antibody (38F3) [NB300-269] - IHC staining of Fibrillarin in human ovarian cancer using DAB with hematoxylin counterstain.
Flow Cytometry: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin antibody was tested at 1:400 in HEK293 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).
Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Human SH-SY5Y cells stained with NB300-269, showing prominent specular nucleolar staining. The nuclei are counter stained with DAPI (blue), so these spots appear very pale blue.  Neurofilament heavy protein was stained with NB300-217 (red).
Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin immunofluorescence in fibroblasts. Image from verified customer review.
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Ordering Information: NB300-269

  • Catalog Number
    NB300-269
  • Displayed Format
    Unconjugated
  • Size(s)
    0.25 ml
  • Availability
  • Price
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Fibrillarin Antibody (38F3) Summary

Species Human, Mouse, Rat, C. elegans, Chicken, Drosophila, Plant, Yeast
Tested Applications WB, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt
Clonality
Monoclonal
Host
Mouse
Gene
FBL
Purity
Tissue culture supernatant
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Fibrillarin Antibody (38F3) Details

Clone
38F3
Isotype
IgG1
Marker
Nucleolar Marker
Immunogen
Yeast nuclear preparation (S. cerevisiae).
Localization
Nucleus > nucleolus. Note: Fibrillar region of the nucleolus.

Species Reactivity

Human, rat, mouse, chicken, C. elegans, Drosophila, plants and yeast. Other species not tested.
Reviewed Species Read 2 rated 5
using NB300-269 in the following species:

Publications Read Publications using NB300-269 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Flow Cytometry 1:20-1:400
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Immunohistochemistry Whole-Mount
Application Notes
This Fibrillarin Antibody (38F3) is useful for Western blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry and Immunohistochemistry on both paraffin-embedded and frozen sections. In WB a band can be seen at approximately 34 kDa. In ICC/IF, this antibody shows prominent specular nucleolar staining. Use in Immunohistochemistry whole mount reported in scientific literature (PMID 24722283)
Molecular Weight
34 kDa
Positive Controls
Reviewed Applications
Read 2 Review rated 5
using
NB300-269 in the following applications:

Publications
Read Publications using
NB300-269 in the following applications:

Contact Information

Product PDFs

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Reviews

Average Rating: 5
Recent Reviews
ICC/IF Tested In Human
Reviewed On: 06/09/2014
Rated: 5

Fibrillarin NB300-269
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Publications

Fibrillarin Antibody (NB300-269) has been mentioned in at least 4 publications.
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Unit Size
0.25 ml
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Preservative
10mM Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol FBL
Entrez

Alternate Names for Fibrillarin Antibody (38F3)

  • EC 2.1.1,34-kD nucleolar scleroderma antigen
  • EC 2.1.1.-
  • EC 2.1.1.37
  • FIB
  • FIB1,34 kDa nucleolar scleroderma antigen
  • fibrillarin
  • FLRNrRNA 2'-O-methyltransferase fibrillarin
  • RNA, U3 small nucleolar interacting protein 1
  • RNU3IP1
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Related Products by Gene

Background

Fibrillarin / Nop1p, was originally identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is Fibrillarin (accession P22087), a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human Fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the Fibrillarin domain. A Fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout the entire Fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be Fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Reviews for Fibrillarin Antibody (NB300-269) (2) 52

Read what people are saying who have used Fibrillarin Antibody (NB300-269).

We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1: Immunocytochemistry/Immunofluorescence.
Average Rating: 5
(Based on 2 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Fibrillarin NB300-269
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Excellent
ICC/IF Human Anonymous 06/09/2014
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
SpeciesHuman
Special ApplicationsIF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Immunocytochemistry/Immunofluorescence Fibrillarin NB300-269
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Excellent
ICC/IF Human Anonymous 04/24/2014
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedFibroblasts
SpeciesHuman
Lot92412

Blocking

Blocking Detailsblocked in 5% Normal Goat serum/0.3% Triton X-100 in PBS, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:500, 4C, overnight, in 1X PBS/1% BSA/0.3% Triton X-100

Secondary Antibody

Secondary Manufacturer Cat#cell signaling 4408
Secondary Concentration1:1000

Details

Fixation DetailsFixed in PFA, Blocked in Normal Goat serum containing Triton X-100, Incubated overnight 1:100 in 1% BSA
Wash Description1X PBS, 3 washes 5 minutes each

Publications for Fibrillarin Antibody (NB300-269) (4)

Read what people are writing who have used Fibrillarin Antibody (NB300-269).

We have publications tested in 3 confirmed species: Human, Mouse, C. elegans.

We have publications tested in 5 applications: WB, ICC/IF, IHC-Fr, IHC-P, IHC-WhMt.

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Product General Protocols

View specific protocols for Fibrillarin Antibody (NB300-269): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for Fibrillarin (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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