Fibrillarin Antibody (38F3) 0.25 ml Nucleolar Marker

Fibrillarin NB300-269

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Ordering Information: NB300-269

Catalog NumberNB300-269
  • FormUnconjugated
  • Size(s)0.25 ml
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Fibrillarin Antibody (38F3) Summary

 
Species Human, Mouse, Rat, C. elegans, Chicken, Drosophila, Plant, Yeast
Tested Applications WB, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality Monoclonal
Host Mouse
Gene FBL
Purity Tissue culture supernatant
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Fibrillarin Antibody (38F3) Details

 
Clone 38F3
Isotype IgG1
Marker Nucleolar Marker
Immunogen Yeast nuclear preparation (S. cerevisiae).
Localization Nucleus > nucleolus. Note: Fibrillar region of the nucleolus.

Species Reactivity

 
Human, rat, mouse, chicken, C. elegans, Drosophila, plants and yeast. Other species not tested.
Publications Read 3 using NB300-269 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 1:1000-1:5000
  • Flow Cytometry 1:20-1:400
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes

This Fibrillarin Antibody (38F3) is useful for Western blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry and Immunohistochemistry on both paraffin-embedded and frozen sections. In WB a band can be seen at approximately 34 kDa. In ICC/IF, this antibody shows prominent specular nucleolar staining.

Molecular Weight

34 kDa

Positive Controls

1 Positive Control

Publications
Read 3 publications using
NB300-269 in the following applications:
  • WB4publications

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Publications

Fibrillarin Antibody (NB300-269) has been mentioned in at least 3 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

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Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Unit Size 0.25 ml
Concentration This product is unpurified. The exact concentration of antibody is not quantifiable.
Preservative 10mM Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol FBL
Entrez

Alternate Names for Fibrillarin

 
  • EC 2.1.1.37
  • EC 2.1.1,34-kD nucleolar scleroderma antigen
  • FIB1,34 kDa nucleolar scleroderma antigen
  • RNU3IP1
  • EC 2.1.1.-
  • FIB
  • FLRNrRNA 2'-O-methyltransferase fibrillarin
  • RNA, U3 small nucleolar interacting protein 1
  • fibrillarin

Related Products by Gene

Background

 
Fibrillarin / Nop1p, was originally identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is Fibrillarin (accession P22087), a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human Fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the Fibrillarin domain. A Fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout the entire Fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be Fibrillarin. Fibrillarin is an excellent marker for the nucleolus.