Fibrillarin Antibody (38F3) Summary
||Human, Mouse, Rat, C. elegans, Chicken, Drosophila, Plant, Yeast
||WB, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt
Tissue culture supernatant
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Fibrillarin Antibody (38F3) Details
Yeast nuclear preparation (S. cerevisiae).
Nucleus > nucleolus. Note: Fibrillar region of the nucleolus.
Human, rat, mouse, chicken, C. elegans, Drosophila, plants and yeast. Other species not tested.
Read 2 rated 5 using NB300-269 in the following species:
||Read Publications using NB300-269 in the following species:
- Western Blot 1:1000-1:5000
- Flow Cytometry 1:20-1:400
- Immunocytochemistry/Immunofluorescence 1:500-1:1000
- Immunohistochemistry 1:500-1:1000
- Immunohistochemistry-Frozen 1:500-1:1000
- Immunohistochemistry-Paraffin 1:500-1:1000
- Immunohistochemistry Whole-Mount
This Fibrillarin Antibody (38F3) is useful for Western blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry and Immunohistochemistry on both paraffin-embedded and frozen sections. In WB a band can be seen at approximately 34 kDa. In ICC/IF, this antibody shows prominent specular nucleolar staining. Use in Immunohistochemistry whole mount reported in scientific literature (PMID 24722283)
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ICC/IF Tested In Human
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Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
This product is unpurified. The exact concentration of antibody is not quantifiable.
10mM Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Alternate Names for Fibrillarin Antibody (38F3)
- EC 2.1.1,34-kD nucleolar scleroderma antigen
- EC 2.1.1.-
- EC 184.108.40.206
- FIB1,34 kDa nucleolar scleroderma antigen
Related Products by Gene
, TRANSCRIPTION FACTOR UBF
Fibrillarin / Nop1p, was originally identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is Fibrillarin (accession P22087), a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human Fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the Fibrillarin domain. A Fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout the entire Fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be Fibrillarin. Fibrillarin is an excellent marker for the nucleolus.
Reviews for Fibrillarin Antibody (NB300-269) (2)
Read what people are saying who have used Fibrillarin Antibody (NB300-269).
We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1: Immunocytochemistry/Immunofluorescence.
Average Rating: 5
(Based on 2 reviews)
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|Special Applications||IF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).|
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|Blocking Details||blocked in 5% Normal Goat serum/0.3% Triton X-100 in PBS, 1 hour, room temperature|
|Dilution Ratio||1:500, 4C, overnight, in 1X PBS/1% BSA/0.3% Triton X-100|
|Secondary Manufacturer Cat#||cell signaling 4408|
|Fixation Details||Fixed in PFA, Blocked in Normal Goat serum containing Triton X-100, Incubated overnight 1:100 in 1% BSA|
|Wash Description||1X PBS, 3 washes 5 minutes each|
Publications for Fibrillarin Antibody (NB300-269) (4)
Read what people are writing who have used Fibrillarin Antibody (NB300-269).
We have publications tested in 3 confirmed species: Human, Mouse, C. elegans.
We have publications tested in 5 applications: WB, ICC/IF, IHC-Fr, IHC-P, IHC-WhMt.
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|Beyret E, Lin H. Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse Dev Biol 2011 Jul 15 [PMID: 21539824] (IHC-Fr, Mouse)
|Zillner K, Filarsky M, Rachow K et al. Large-scale organization of ribosomal DNA chromatin is regulated by Tip5 Nucleic Acids Res 2013 Apr 10 [PMID: 23580549] (ICC/IF, Human)
|Su H, Xu T, Ganapathy S et al. Elevated snoRNA biogenesis is essential in breast cancer Oncogene 2013 Apr 1 [PMID: 23542174] (WB, IHC-P, Human)
|Lee CC, Tsai YT, Kao CW et al. Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans. Cell Death Dis 2014 Apr 11 [PMID: 24722283] (IHC-WhMt, C. elegans)
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FAQs for Fibrillarin (2)
- In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
- For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
- I was wondering what does Format 7C mean as far as anitbodies go?
- 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
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