Fatty Acid Synthase Antibody (NB400-114)
Fatty Acid Synthase Antibody
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Distributor Data...
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anti-Fas antibody, anti-Oleoyl-[acyl-carrier-protein] hydrolase antibody, anti-Enoyl-[acyl-carrier-protein] reductase antibody, anti-Acyl-carrier-protein] S-acetyltransferase antibody, anti-3-oxoacyl-[acyl-carrier-protein] synthase antibody, anti-3-oxoacyl-[acyl-carrier-protein] reductase antibody, anti-3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody, anti-[Acyl-carrier-protein] S-malonyltransferase antibody, |
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Fatty Acid Synthase Antibody Summary: | | Gene: | FASN |
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| Purity: | Whole antisera |
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| Host: | Rabbit |
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| | Specificity: | NB400-114 is specific for FAS protein. |
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| Publications: | Antibody has been mentioned in at least 7 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Fatty Acid Synthase Products Fatty Acid Synthase Antibody Details: | Immunogen: | An internal synthetic peptide, conjugated to KLH, made near the N-terminus of the mouse FAS protein sequence.
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| Localization: | Cytoplasmic |
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NB 400-114 reacts with human, pig, rat, monkey, chicken and mouse FAS. |
| Uses: | NB 400-114 may be used for Western Blot where a band at ~272 kD should be seen on SDS-PAGE. May see 1 or 2 minor cross-reacting lower MW bands in liver tissue. It has also been used for immunohistochemistry on paraffin sections. |
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| Dilutions: | Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation 1:100, Western Blot 1:1000 |
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| TMW: | 273 kDa |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 2 Positive Controls |
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Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Preservative: | 0.05% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: FAS (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis (triglyceride production). It is a target for SREBP and is upregulated by LXR activation. Fatty liver is often the result of FAS activation. It is also one of the accepted markers for insulin resistance and SREBP , LXR activation.
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Images (3) Reviews (1) Related Products (21) Exploring Fatty Acid Synthase Working with Fatty Acid Synthase
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with Fatty Acid Synthase
Fatty Acid Synthase Antibody Images (3) Western Blot: Fatty Acid Synthase antibody [NB400-114] - Expression of fatty acid synthase (FAS) in the chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, and liver and muscle, which were chosen as positive controls) was subjected to RT-PCR using specific primers for chicken FAS (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, and liver and muscle, which were chosen as positive controls) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-FAS antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2. "FAS inhibitor cerulenin reduces food intake and melanocortin receptor gene expression without modulating the other (an)orexigenic neuropeptides in chickens" |  Immunohistochemistry: Fatty Acid Synthase Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens. |  Western Blot: Fatty Acid Synthase Antibody [NB400-114] - Western blot analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate. |
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Reviewed by:
Chiung-Chi Peng 12/6/2011
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Application:
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Western Blot
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Sample Tested:
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Rat
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Species:
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Rat
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Sample Pretreated:
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Doxorubicin
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Dilution Ratio:
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1:1000
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Incubation Time:
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Overnight
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Publications (7)Fatty Acid Synthase Antibody Product Specific:- Basciano H, et al. Metabolic effects of dietary cholesterol in an animal model of insulin resistance and hepatic steatosis. Am J Physiol Endocrinol Metab. 2009 Aug;297(2):E462-73. Epub 2009 Jun 9. [pubmed: 19509184] (Hamster, WB)
- Basciano H, Miller A, Baker C, et al. LXR{alpha} activation perturbs hepatic insulin signaling stimulates production of apolipoprotein B-containing lipoproteins. Am J Physiol Gastrointest Liver Physiol 2009;297(2):G323-332. [pubmed: 19497957]
more... - Gao H, Bryzgalova G, Hedman E, et al. Long-term administration of estradiol decreases expression of hepatic lipogenic genes and improves insulin sensitivity in ob/ob mice: a possible mechanism is through direct regulation of signal transducer and activator of transcription 3. Mol Endocrinol. 2006 Jun;20(6):1287-99. [PMID: 16627594]
- Bryzgalova G, Lundholm L, Portwood N,et al. Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed mice. Am J Physiol Endocrinol Metab. 2008 Oct;295(4):E904-12. [PMID: 18697913]
- Vavere, AL, et al. 1-11C-acetate as a PET radiopharmaceutical for imaging fatty acid synthase expression in prostate cancer. J Nucl Med. 2008 Feb;49(2):327-34. Epub 2008 Jan 16.
- Heemers, HV, et al. Androgen deprivation increases p300 expression in prostate cancer cells. Cancer Res. 2007 Apr 1;67(7):3422-30.
- Dridi S, et al. FAS inhibitor cerulenin reduces food intake and melanocortin receptor gene expression without modulating the other (an)orexigenic neuropeptides in chickens. Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2. Erratum in: Am J Physiol Regul Integr Comp Physiol. 2006 Sep;291(3):R860. Lutgarde, Arckens [corrected to Arckens, Lutgarde]. [PMID: 16455759]
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Related ProductsPositive ControlsAntibody Pairs (2)Lysates (2)Peptides and ProteinsPrimary Antibodies (13)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Fatty Acid Synthase Antibody (3F2-1F3) | H00002194-M01 | Hu | ELISA, IF, IHC-P, WB | (4) | (1) | | | | Fatty Acid Synthase Antibody | NB100-87029 | Hu, Mu | IF, IHC-P | (3) | | | | | Fatty Acid Synthase Antibody | NBP1-84733 | Hu | IF, IHC-P, WB | (3) | | | | | Fatty Acid Synthase Antibody | H00002194-B01P | Hu | ELISA, IF, WB | (3) | | | | | Fatty Acid Synthase Antibody (3B3-1D6) | H00002194-M02 | Hu | ELISA, IF, WB | (2) | | | | | ... and 8 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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