Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide corresponding to residues R(a.a. 224) K N V K Q H A D Q T V N E D(a.a. 238) of mouse FXR.
Species Reactivity:
Mouse.
Applications:
Uses:
Western Blot. By WB, this antibody detects an approx. 55 kDa protein representing FXR in mouse liver samples. An unknown band at approx. 60 kDa is also observed in western blots.
Dilutions:
Western Blot 1:500
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Packaging:
Storage:
Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:
PBS containing 1 mg/ml BSA
Preservative:
0.05% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Bile Acid Receptor NR1H4 is a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. It represses the transcription of the cholesterol 7 alpha hydroxylase gene and activates the intestinal bile acid binding protein. The NR1H4 gene encodes a protein that has a central role in lipid homeostasis and also regulates carbohydrate metabolism.
Western Blot: FXR Antibody [NB100-74420] - Figure 1 illustrates Western blot detection of FXR from 50 ug of mouse liver extract. Lane 1 is incubated with NB100-74420 (1:500), and lane 2 has the antibody plus the neutralizing peptide (PEP-273).