Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide corresponing to the residues surrounding Asparagine 259 of human FOXO3A
Species Reactivity:
Human, Mouse, Rat
Applications:
Uses:
This antibody is useful for Western Blot and Immunohistochemistry. Western blot analyzes of FOXO3A (N259) antibody in extracts from NIH/3T3 cells. Immunohistochemistry analyzes of FOXO3A (N259) antibody in paraffin-embedded human breast carcinoma tissue.
Dilutions:
Immunohistochemistry-Paraffin 1:50-1:200, Western Blot 1:500-1:1000
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Phosphate buffered saline (PBS), approx. pH 7.2.
Preservative:
15mM Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors. It recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. FOXO3a has been shown to induce cell growth arrest to effect tumor suppression and FOXO3A genotype is strongly associated with human longevity.