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FOXO3A Antibody (NB100-81928)

FOXO3A Antibody

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FOXO3A Antibody Summary:
Species:Hu, Mu, Rt
Applications:IHC, WB
Clonality:Polyclonal
Gene:FOXO3
Purity:Immunogen affinity purified
Host:Rabbit
Specificity:FKHRL1 (specific to a.a. 253) detects endogenous levels of total FKHR1 protein.
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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FOXO3A Antibody Details:
Isotype:IgG
Immunogen:The antiserum was produced against synthesized non-phosphopeptide derived from human FKHR1 around the phosphorylation site of serine 253 (A-V-SP-M-D).
Species Reactivity:

This antibody is reactive against Human, Mouse, Rat

Applications:
Uses:This antibody is useful for: Western Blot immunohistochemistry
Dilutions:Western Blot, immunohistochemistry,
Unit Size:0.1 mg
Concentration:1.0 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: FOXO3
Entrez2309 (Human)
56484 (Mouse)
294515 (Rat)
Swiss ProtO43524 (Human)
 
Background:

FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Images (2)
  • Western Blot: FKHRL1 Antibody [NB100-81928] - Western blot analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (Lane 3 and 4).
  • Immunohistochemistry: FKHRL1 Antibody [NB100-81928] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (Ab-253) antibody.

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Working with FOXO3A

FOXO3A Antibody Images (2)
Western Blot: FKHRL1 Antibody [NB100-81928] - Western blot analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (Lane 3 and 4).Immunohistochemistry: FKHRL1 Antibody [NB100-81928] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (Ab-253) antibody.
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Publications (1)

FOXO3A Antibody Background References:

  1. Smith WW, et al. (2005) J Cell Biol; 169(2): 331-9. Essafi A, et al. (2005) Oncogene; 24(14): 2317-29. Brunet A, et al. (2001) Mol Cell Biol; 21(3): 952-65. Brunet A, et al. (1999) Cell; 96(6): 857-68.
Protocols
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FOXO3A in Context

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Novus Explorer for FOXO3A gene
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