Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide: GAKQASSQSWVPG, representing the C-Terminus of the human protein (residues 661-673), according to NP_001446.
Localization:
Nuclear
Species Reactivity:
Human, Mouse, Rat, Dog, Pig, Cow
Applications:
Uses:
Peptide ELISA and Western Blot. In WB an approx 90kDa band observed in human Brain and Heart lysates (calculated MW of 71.3kDa according to NP_001446 and NP_963853). Recommended concentration: 0.1-0.3µg/ml.
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Tris-saline (pH7.3) with 0.5% BSA
Preservative:
0.02% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
NB 100-1438 staining (0.5µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.