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![Western Blot: Egr1 Antibody [NB600-873] - Western blot using Affinity Purified anti-EGR-1 antibody shows detection of a predominant band at ~58 kDa corresponding to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gta- Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/images/Egr1 Antibody-Western Blot-NB600-873-img0002.jpg "Western Blot: Egr1 Antibody [NB600-873] - Western blot using Affinity Purified anti-EGR-1 antibody shows detection of a predominant band at ~58 kDa corresponding to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gta- Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Western Blot: Egr1 Antibody [NB600-873] - NB600-873 Western blot - Egr1 antibody](http://images.novusbio.com/images/Egr1 Antibody-Western Blot-NB600-873-img0003.jpg "Western Blot: Egr1 Antibody [NB600-873] - NB600-873 Western blot - Egr1 antibody")
![Immunohistochemistry: Egr1 Antibody [NB600-873] - Immunohistochemistry. Affinity Purified anti-EGR-1 antibody was used at a 10 ug/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/images/Egr1 Antibody-Immunohistochemistry-NB600-873-img0001.jpg "Immunohistochemistry: Egr1 Antibody [NB600-873] - Immunohistochemistry. Affinity Purified anti-EGR-1 antibody was used at a 10 ug/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.")
