Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The epitope recognized by this antibody maps to a region between residue 310 and 360 of human extracellular signal-regulated kinase-2 (mitogen-activated protein kinase 1) using the numbering given in entry NP_002736.3 (GeneID 5594).
Species Reactivity:
Human
Applications:
Uses:
This antibody is useful for western blot and Immunohistochemistry-Paraffin. It is not recommended for immunoprecipitation.
Dilutions:
Immunohistochemistry-Paraffin, Western Blot 1:2000-1:10000
Unit Size:
0.1 ml
Concentration:
1.0 mg/ml
Notes:
Immunohistochemistry-Paraffin Image 2 Notes: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERK2 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
Tris-citrate/phosphate buffer, pH 7 to 8
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq]
Western Blot: ERK2 Antibody [NBP1-19102]-Whole cell lysate from HeLa (5, 15 and 50 mcg), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Antibody: Affinity purified rabbit anti-ERK2 antibody used for WB at 0.1 mcg/ml.
Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-19102] - Please see notes section