ERK2 Antibody (NB300-920)

ERK2 Antibody

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ERK2 Antibody Summary:
Species:Hu
Applications:PEP-ELISA, WB
Clonality:Polyclonal
Gene:MAPK1
Purity:Immunogen affinity purified
Host:Goat
Specificity:This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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ERK2 Antibody Details:
Isotype:IgG
Immunogen:Synthetic peptide CAAGPEMVRGQVF, representing the internal region of the protein sequence according to NP_002736; NP_620407.
Species Reactivity:

Human, Mouse, Rat, Dog, Cow

Applications:
Uses:This antibody is useful for Peptide ELISA and Western Blot. Approx 38kDa band observed in human cervix epitheloid carcinoma HeLa and and human hepatoblastoma HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407).
Dilutions:Peptide ELISA 1:2000, Western Blot 1:1000
Unit Size:0.1 mg
Concentration:0.5 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:Tris-saline (pH7.3) with 0.5% BSA
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: MAPK1
Entrez5594 (Human)
26413 (Mouse)
Swiss ProtNP_002736; NP_620407.
 
Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

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Working with ERK2

ERK2 Antibody Images (1)
NB 300-920 (0.5ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Publications (1)

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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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ERK2 in Context


Research Areas related to ERK2 (8):
Novus Explorer for ERK2 gene

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