ERK2 Antibody (NB110-57174)

ERK2 Antibody (E460)

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ERK2 Antibody (E460) Summary:
Species:Hu, Mu, Rt
Applications:FACS, ICC, IHC-P, IP, WB
Clonality:Monoclonal
Gene:MAPK1
Purity:IgG purified
Host:Rabbit
 
Publications:Antibody has at least 2 Background References.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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ERK2 Antibody (E460) Details:
Clone:E460
Isotype:IgG
Immunogen:A synthetic peptide corresponding to residues near the C-term of human ERK2 p42 MAPK was used as immunogen.
Species Reactivity:

Reacts in Human, Mouse, Rat.

Applications:
Uses:This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 42kDa.
Dilutions:Western Blot 1:1000-2000, Immunohistochemistry-Paraffin 1:250-500, Immunocytochemistry 1:100-250, Immunoprecipitation 1:80, Flow Cytometry 1:50
TMW:42 kDa
Unit Size: 0.1 ml
Concentration:Please see the vial label for concentration.
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA.
Preservative:0.01% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: MAPK1
Entrez5594 (Human)
26413 (Mouse)
116590 (Rat)
Swiss ProtP28482 (Human)
 
Background:

Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen

 
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Working with ERK2

ERK2 Antibody (E460) Images (2)
Immunohistochemistry: ERK2 p42 MAPK (C-term) Antibody (E460) [NB110-57174] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti- ERK2 p42 MAPK (C-term) (cat. # 110-57174).Western Blot: ERK2 p42 MAPK (C-term) Antibody (E460) [NB110-57174] - Western blot analysis on A431 cell lysate using anti-ERK2 p42 MAPK (C-term) (cat. #110-57174), 1:2,000 dilution
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Publications (2)

ERK2 Antibody (E460) Background References:

  1. Chen RH, Sarnecki C Blenis J (1992). Mol Cell Biol, 12, 915
  2. Cobb, M. H.; Boulton, T. G.; Robbins, D. J. Cell Regul. 2: 965-978, 1991.

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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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ERK2 in Context


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