ERK1 [p Thr202, p Tyr204] Antibody (NB500-141)

ERK1 [p Thr202, p Tyr204] Antibody (NB500-141) (0.1 ml)

Additional Products Available View our ERK1 products. Call 303-730-1950 for recommended products	Ordering Information:
	Catalog Number	NB500-141
	Size(s)	0.1 ml	Price:
	Availability
Why Choose Novus?	Bulk Quote
	View All ERK1 Primary Antibodies

anti-EC 2.7.11 antibody, anti-MAPK 3 antibody, anti-extracellular signal-related kinase 1 antibody, anti-MAPK 1 antibody, anti-P44MAPK antibody, anti-MAP kinase isoform p44 antibody, anti-P44ERK1 antibody, anti-MAP kinase 3 antibody, anti-MAP kinase 1 antibody, anti-ERT2 antibody, anti-MGC20180 antibody, anti-ERK-1 antibody, anti-HUMKER1A antibody, anti-Mitogen-activated protein kinase 1 antibody, anti-HS44KDAP antibody, anti-Microtubule-associated protein 2 kinase antibody, anti-p44mapk antibody, anti-Insulin-stimulated MAP2 kinase antibody, anti-p44erk1 antibody, anti-Extracellular signal-regulated kinase 1 antibody, anti-PRKM3EC 2.7.11.24 antibody, anti-ERK1p44-MAPK antibody, anti-p44-ERK1 antibody, anti-mitogen-activated protein kinase 3 antibody

ERK1 [p Thr202, p Tyr204] Antibody Summary:

Species:	Hu, Rt	Gene:	MAPK3
Applications:	WB, IHC-P	Purity:	Affinity purified
Clonality:	Polyclonal	Host:	Rabbit
Specificity:	Specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.
Publications:

View Additional ERK1 Products

Antibody Pairs	Lysates
Peptides and Proteins	Primary Antibodies
RNAi
» View All ERK1 Related Products

ERK1 [p Thr202, p Tyr204] Antibody Details:

Immunogen:	Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH
Localization:	Cell Membrane
Modification:	p Thr202, p Tyr204

Species Reactivity:

Tested in Human and Rat. Reactivity assumed based on 100% sequence homology: Bovine, Canine, Chicken, Mouse, Primate, Xenopus and Zebrafish.

Applications:

Uses:	NB 500-141 can be used in Western blot where a band is seen at ~ 42-44 kDa, representing ERK 1/2.
Dilutions:	Immunohistochemistry-Paraffin, Western Blot 1:1000
Unit Size:	0.1 ml
Concentration:	Please see the vial label for concentration.

Packaging:

Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Buffer:	10mM HEPES [pH 7.5], 150 mM NaCl, 100 ug/ml BSA and 50% glycerol.
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: MAPK3

Entrez:	5595 (Human)
	26417 (Mouse)
	50689 (Rat)
Uniprot:	P63086 (Rat)

Related Genes:

Background:

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases (p44 and p42), designated ERK 1 (extracellular-signal related kinase 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites, which is required for full enzymatic activation. Once activated, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MEK and Raf-1.

Images (1)


View All Images...

Be the first to review this product for a reward!

Review this product now.

ERK1 [p Thr202, p Tyr204] Antibody Images (1)

Western Blot: ERK/MAPK Phosphospecific Antibody [NB500-141] - Western blot of human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr202/Tyr204 ERK/MAPK. The immunolabeling is completely eliminated by treatment with lambda-Ptase.