ERK1 Antibody (NB500-141)
ERK1 [p Thr202, p Tyr204] Antibody
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Distributor Data...
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Anti-ERK 1 antibody; Anti-ERK 2 antibody; Anti-ERT1 antibody; Anti-ERT2 antibody; Anti-Extracellular signal regulated kinase 1 antibody; Anti-Extracellular signal regulated kinase 2 antibody; Anti-HS44KDAP antibod; Anti-HUMKER1A antibody; Anti-Insulin sti, |
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ERK1 [p Thr202, p Tyr204] Antibody Summary: | | Gene: | MAPK3 |
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| Purity: | Affinity purified |
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| Host: | Rabbit |
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| | Specificity: | Specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional ERK1 Products ERK1 [p Thr202, p Tyr204] Antibody Details: | Immunogen: | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH |
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| Localization: | Cell Membrane |
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| Modification: | p Thr202, p Tyr204 |
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Human and Rat. Expected to react based on homology: Bovine, Canine, Chicken, Mouse, Primates, Xenopus and Zebra Fish |
| Uses: | NB 500-141 can be used in Western blot where a band is seen at ~ 42-44 kDa, representing ERK 1/2. |
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| Dilutions: | Immunohistochemistry-Paraffin, Western Blot 1:1000 |
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| Unit Size: | 0.1 ml |
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| Concentration: | Please see the vial label for concentration. |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 10mM HEPES [pH 7.5], 150 mM NaCl, 100 ug/ml BSA and 50% glycerol. |
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| Preservative: | No Preservative |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases (p44 and p42), designated ERK 1 (extracellular-signal related kinase 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites, which is required for full enzymatic activation. Once activated, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MEK and
Raf-1.
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Images (1) Reviews (0) Related Products (45) Exploring ERK1
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Working with ERK1
ERK1 [p Thr202, p Tyr204] Antibody Images (1) Western Blot: ERK/MAPK Phosphospecific Antibody [NB500-141] - Western blot of human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr202/Tyr204 ERK/MAPK. The immunolabeling is completely eliminated by treatment with lambda-Ptase. |
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Jump to: Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsAntibody Pairs (1)Lysates (2)Peptides and ProteinsPrimary Antibodies (22)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ERK1 Antibody (3C9) | H00005595-M01 | Hu | ELISA, IF, IHC-P, WB | (5) | | | | | ERK1 Antibody (Y72) | NB110-56979 | Hu | FACS, ICC, IHC-P, IP, WB | (2) | (1) | | | | ERK1 Antibody | NBP1-84807 | Hu | IF, IHC-P, WB | (2) | | | | | ERK1 Antibody | NBP1-84808 | Hu | IF, IHC-P, WB | (2) | | | | | ERK1 Antibody | NB100-1942 | Hu, Mu, Rt | IHC-P, IP, WB | (1) | | | | | ... and 17 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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