ERK1 Antibody (Y72) (NB110-56979)

ERK1 Antibody (Y72) (NB110-56979) (0.1 ml)

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	Catalog Number	NB110-56979
	Size(s)	0.1 ml	Price:
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anti-EC 2.7.11 antibody, anti-MAPK 3 antibody, anti-extracellular signal-related kinase 1 antibody, anti-MAPK 1 antibody, anti-P44MAPK antibody, anti-MAP kinase isoform p44 antibody, anti-P44ERK1 antibody, anti-MAP kinase 3 antibody, anti-MAP kinase 1 antibody, anti-ERT2 antibody, anti-MGC20180 antibody, anti-ERK-1 antibody, anti-HUMKER1A antibody, anti-Mitogen-activated protein kinase 1 antibody, anti-HS44KDAP antibody, anti-Microtubule-associated protein 2 kinase antibody, anti-p44mapk antibody, anti-Insulin-stimulated MAP2 kinase antibody, anti-p44erk1 antibody, anti-Extracellular signal-regulated kinase 1 antibody, anti-PRKM3EC 2.7.11.24 antibody, anti-ERK1p44-MAPK antibody, anti-p44-ERK1 antibody, anti-mitogen-activated protein kinase 3 antibody

ERK1 Antibody (Y72) Summary:

Species:	Hu	Gene:	MAPK3
Applications:	WB, FACS, ICC, IHC-P, IP	Purity:	IgG purified
Clonality:	Monoclonal	Host:	Rabbit
Specificity:	The antibody does not cross-react with other MAP kinases.
Publications:	NB110-56979 has at least 1 Background Reference

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ERK1 Antibody (Y72) Details:

Clone:	Y72
Isotype:	IgG
Immunogen:	A synthetic peptide corresponding to residues near N-terminus of human ERK1 was used as immunogen.
Epitope:	Reacts with an epitope located in the N terminal region of ERK1.

Species Reactivity:

Human. Mouse and Rat cross reactivity tested by Western Blot only.

Applications:

Uses:	This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 44kDa.
Dilutions:	Western Blot 1:1000-5000, Immunohistochemistry-Paraffin 1:100, Immunocytochemistry 1:100, Immunoprecipitation 1:40, Flow Cytometry 1:40
Unit Size:	0.1 ml
Concentration:	Please see the vial label for concentration.
Positive Controls:	2 Positive Controls

Packaging:

Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Buffer:	40% Glycerol, 0.05% BSA, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4
Preservative:	0.01% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: MAPK3

Entrez:	5595 (Human)
	26417 (Mouse)
	50689 (Rat)
Uniprot:	P27361 (Human)

Related Genes:

Background:

p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

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ERK1 Antibody (Y72) Images (2)

	Western Blot: p44 MAPK(Erk1) Antibody (Y72) [NB110-56979] - Western blot analysis on Jurkat cell lysate using anti-p44 MAPK (Erk1) (N-term) (cat. #110-56979); dilution 1:1,000.

	Immunohistochemistry: p44 MAPK(Erk1) Antibody (Y72) [NB110-56979] - Immunohistochemical analysis of paraffin-embedded lung carcinoma using anti-p44 MAPK (Erk1) (N-term) (cat. #110-56979).