ERK1 Antibody (NB110-56979)

ERK1 Antibody (Y72)

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ERK1 Antibody (Y72) Summary:
Species:Hu
Applications:FACS, ICC, IHC-P, IP, WB
Clonality:Monoclonal
Gene:MAPK3
Purity:IgG purified
Host:Rabbit
Specificity:The antibody does not cross-react with other MAP kinases.
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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ERK1 Antibody (Y72) Details:
Clone:Y72
Isotype:IgG
Immunogen:A synthetic peptide corresponding to residues near N-terminus of human ERK1 was used as immunogen.
Epitope:Reacts with an epitope located in the N terminal region of ERK1.
Species Reactivity:

Reacts in Human.

Applications:
Uses:This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 44kDa.
Dilutions:Western Blot 1:1000-5000, Immunohistochemistry-Paraffin 1:100, Immunocytochemistry 1:100, Immunoprecipitation 1:40, Flow Cytometry 1:40
TMW:44 kDa
Unit Size:0.1 ml
Concentration:Please see the vial label for concentration.
Positive Controls:2 Positive Controls
Packaging:
Storage:Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:40% Glycerol, 0.05% BSA, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4
Preservative:0.01% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: MAPK3
Entrez5595 (Human)
26417 (Mouse)
50689 (Rat)
Swiss ProtP27361 (Human)
 
Background:

p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

 
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Working with ERK1

ERK1 Antibody (Y72) Images (2)
Immunohistochemistry: p44 MAPK(Erk1) Antibody (Y72) [NB110-56979] - Immunohistochemical analysis of paraffin-embedded lung carcinoma using anti-p44 MAPK (Erk1) (N-term) (cat. #110-56979).Western Blot: p44 MAPK(Erk1) Antibody (Y72) [NB110-56979] - Western blot analysis on Jurkat cell lysate using anti-p44 MAPK (Erk1) (N-term) (cat. #110-56979); dilution 1:1,000.
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Publications (1)

ERK1 Antibody (Y72) Background References:

  1. Charest, D.L, et al. Molecular cloning, expression, characterization of the human mitogen-activated protein kinase p44erk1. Mol Cell Biol. 13: 4679

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Jump to: Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Positive Controls
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
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Lysates (2)
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RNAi (1)
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Secondary Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Rabbit IgG Antibody [HRP]NB730-HRbELISA, ICC, IHC-P, WB(5)

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ERK1 in Context


Research Areas related to ERK1 (9):
Novus Explorer for ERK1 gene

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