ERK1/2 Antibody Summary:

Species:	Hu, Mu, Rt
Applications:	ELISA, IHC-P, WB
Clonality:	Polyclonal

Gene:	MAPK3
Purity:	Affinity purified
Host:	Rabbit

Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.

View Additional ERK1/2 Products

Primary Antibodies

» View all ERK1/2 Related Products

ERK1/2 Antibody Details:

Isotype:	IgG
Immunogen:	Synthetic peptide corresponing to the residues surrounding Leucine 352 of human ERK1/2

Species Reactivity:

Human, Mouse and Rat.

Applications:

Uses:	ELISA, Western Blot and Immunohistochemistry
Dilutions:	ELISA 1:10000-1:20000, Immunohistochemistry-Paraffin 1:50-1:200, Western Blot 1:500-1:1000
Unit Size:	0.1 mg
Concentration:	1.0 mg/ml

Packaging:

Storage:	Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:	Phosphate buffered saline, pH 7.2.
Preservative:	15mM Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: MAPK3

Entrez	5595 (Human)
	50689 (Rat)

Background:

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos- phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Images (2)

See all images...

Reviews (0)

Be the first to review this product for a reward!

Working with ERK1/2

ERK1/2 Antibody Images (2)

Immunohistochemistry: ERK1/2 Antibody [NB100-91754] - ERK1/2 antibody (L352) [NB100-91754] - in paraffin-embedded human breast carcinoma tissue.

Western Blot: ERK1/2 antibody (L352) [NB100-91754] - in extracts from COLO205 cells.

Reviews (0) Have you used this product? Review this product to earn Novus Rewards.

Protocols

All General Protocols

Ask a Scientist

Can't find an answer to your question about ERK1/2? Ask us by using live chat or by submitting your questions below:

Jump to: Primary Antibodies, Secondary Antibodies

Related Products

Primary Antibodies (18)

Name	Catalog#	Reactivity	Applications	Images	Publications
ERK1/2 Antibody	NB110-96887	Bv, Ch, Dr, Hu, Mu, Rt, Sh, Xp	FACS, IF, IHC-Fr, IHC-P, WB	(1)	(5)
ERK1/2 Antibody (B20-U)	NBP1-30128	Hu, Mu, Rt	ELISA, IHC-Fr, IHC-P, IP, WB
ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (E337)	NB110-56969	Hu, Mu(-), Rt(-)	ICC, IHC-P, WB	(2)	(1)
ERK1/2 Antibody	NB100-82100	Hu, Mu, Rt	IF, IHC-P, WB	(2)	(1)
ERK1/2 Antibody	NBP1-80930	Hu	IF, IHC-P, WB	(1)
... and 13 more. See All »

Secondary Antibodies

Name	Catalog#	Reactivity	Applications	Images	Publications
Rabbit IgG Antibody [HRP]	NB730-H	Rb	ELISA, ICC, IHC-P, WB		(5)
Rabbit IgG Antibody [FITC]	NB730-F	Rb	ICC, IHC-P		(1)
Rabbit IgG Antibody [Biotin]	NB730-B	Rb	ELISA, ICC, IHC-P, WB
Rabbit IgG, H/L Chains Antibody [DyLight 488]	NBP1-72944	Rb	FLISA, IF, WB	(3)
Rabbit IgG Antibody [Alkaline Phosphatase]	NB730-AP	Rb	ELISA, ICC, IHC-P, WB		(1)
See All »

Jump to: Novus Explorer, Research Areas

ERK1/2 in Context

Research Areas related to ERK1/2 (8):

Novus Explorer for ERK1/2 gene

Try our bioinformatic tool that shows the relationships between MAPK3 and other genes, diseases, pathways and post-translational modifications.

ERK1/2 Antibody (NB100-91754)

ERK1/2 Antibody

This Product Has Been Discontinued