ERK1/2 [Thr202] Antibody Summary:

Species:	Hu, Mu, Rt
Applications:	IHC-P, WB
Clonality:	Polyclonal

Gene:	MAPK3
Purity:	Immunogen affinity purified
Host:	Rabbit

Specificity:	p44/42 MAP Kinase (specific to Phospho-Thr202) detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at threonine 202
Publications:	Antibody has at least 1 Background Reference.

Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.

View Additional ERK1/2 Products

Primary Antibodies

» View all ERK1/2 Related Products

ERK1/2 [Thr202] Antibody Details:

Isotype:	IgG
Immunogen:	The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of threonine 202 (F-L-TP-E-Y).
Modification:	Thr202

Species Reactivity:

This antibody is reactive against Human, Mouse, Rat

Applications:

Uses:	This antibody is useful for: Western Blot 1:500-1:1000 immunohistochemistry-Paraffin 1:50-1:100
Dilutions:	Immunohistochemistry-Paraffin, Western Blot 1:500-1:1000
Unit Size:	0.05 mg
Concentration:	1.0 mg/ml
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:	phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative:	0.02% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: MAPK3

Entrez	5595 (Human)
	26417 (Mouse)
	50689 (Rat)
Swiss Prot	P27361 (Human)

Background:

p44 MAP kinase aka ERK1 is ubiquitously expressed in various tissues and cells. It is activated by phosphorylation of neighbouring serine and threonine residues by MEK1 and MEK2 in response to diverse stimuli such as growth factors and mitogens.

Images (2)

See all images...

Reviews (0)

Be the first to review this product for a reward!

Working with ERK1/2

ERK1/2 [Thr202] Antibody Images (2)

Western Blot: p44/42 MAP Kinase Phosphospecific Antibody [NB100-82101] - Western blot analysis of extracts from MCF7, 293, A431, A2780 and Hela cells, using p44/42 MAP Kinase antibody (Lanes 1 2 3 4 ) and p44/42 MAP Kinase (phospho-Thr202) antibody (Lanes 5 6 7 8).

Immunohistochemistry: p44/42 MAP Kinase Phosphospecific Antibody [NB100-82101] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (phospho-Thr202) antibody

Reviews (0) Have you used this product? Review this product to earn Novus Rewards.

Publications (1)

ERK1/2 [Thr202] Antibody Background References:

TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397 Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162 Jingui Yu, et al. (2005) Anesth Analg 101: 315-321 Hironobu Ihn et al.(2000) Immunology 165: 2149-2155

Protocols

All General Protocols

Ask a Scientist

Can't find an answer to your question about ERK1/2? Ask us by using live chat or by submitting your questions below:

Jump to: Positive Controls, Primary Antibodies, Secondary Antibodies

Related Products

Positive Controls

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
ERK1 Lysate	NBL1-12877		WB	(1)

Primary Antibodies (18)

Name	Catalog#	Reactivity	Applications	Images	Publications
ERK1/2 Antibody	NB110-96887	Bv, Ch, Dr, Hu, Mu, Rt, Sh, Xp	FACS, IF, IHC-Fr, IHC-P, WB	(1)	(5)
ERK1/2 Antibody (B20-U)	NBP1-30128	Hu, Mu, Rt	ELISA, IHC-Fr, IHC-P, IP, WB
ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (E337)	NB110-56969	Hu, Mu(-), Rt(-)	ICC, IHC-P, WB	(2)	(1)
ERK1/2 Antibody	NB100-82100	Hu, Mu, Rt	IF, IHC-P, WB	(2)	(1)
ERK1/2 Antibody	NBP1-80930	Hu	IF, IHC-P, WB	(1)
... and 13 more. See All »

Secondary Antibodies

Name	Catalog#	Reactivity	Applications	Images	Publications
Rabbit IgG Antibody [HRP]	NB730-H	Rb	ELISA, ICC, IHC-P, WB		(5)
Rabbit IgG Antibody [FITC]	NB730-F	Rb	ICC, IHC-P		(1)
Rabbit IgG Antibody [Biotin]	NB730-B	Rb	ELISA, ICC, IHC-P, WB
Rabbit IgG, H/L Chains Antibody [DyLight 488]	NBP1-72944	Rb	FLISA, IF, WB	(3)
Rabbit IgG Antibody [Alkaline Phosphatase]	NB730-AP	Rb	ELISA, ICC, IHC-P, WB		(1)
See All »

Jump to: Novus Explorer, Research Areas

ERK1/2 in Context

Research Areas related to ERK1/2 (9):

Novus Explorer for ERK1/2 gene

Try our bioinformatic tool that shows the relationships between MAPK3 and other genes, diseases, pathways and post-translational modifications.

ERK1/2 Antibody (NB100-82101)

ERK1/2 [Thr202] Antibody

This Product Has Been Discontinued