ERK1/2 Antibody (NB100-82100) | Novus Biologicals

ERK1/2 Antibody (NB100-82100) (0.05 mg)

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	Catalog Number	NB100-82100
	Size(s)	0.05 mg	Price:
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anti-EC 2.7.11 antibody, anti-MAPK 3 antibody, anti-extracellular signal-related kinase 1 antibody, anti-MAPK 1 antibody, anti-P44MAPK antibody, anti-MAP kinase isoform p44 antibody, anti-P44ERK1 antibody, anti-MAP kinase 3 antibody, anti-MAP kinase 1 antibody, anti-ERT2 antibody, anti-MGC20180 antibody, anti-ERK-1 antibody, anti-HUMKER1A antibody, anti-Mitogen-activated protein kinase 1 antibody, anti-HS44KDAP antibody, anti-Microtubule-associated protein 2 kinase antibody, anti-p44mapk antibody, anti-Insulin-stimulated MAP2 kinase antibody, anti-p44erk1 antibody, anti-Extracellular signal-regulated kinase 1 antibody, anti-PRKM3EC 2.7.11.24 antibody, anti-ERK1p44-MAPK antibody, anti-p44-ERK1 antibody, anti-mitogen-activated protein kinase 3 antibody

ERK1/2 Antibody Summary:

Species:	Hu, Mu, Rt	Gene:	MAPK3
Applications:	WB, IF, IHC-P	Purity:	Immunogen affinity purified
Clonality:	Polyclonal	Host:	Rabbit
Specificity:	p44/42 MAP Kinase (specific to Phospho-Tyr204) detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at tyrosine 204
Publications:	NB100-82100 has at least 1 Background Reference

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ERK1/2 Antibody Details:

Isotype:	IgG
Immunogen:	The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-YP-V-A).

Species Reactivity:

This antibody is reactive against Human, Mouse, Rat

Applications:

Uses:	This antibody is useful for: Western Blot 1:500-1:1000
Dilutions:	Immunofluorescence 1:100-1:200, Immunohistochemistry-Paraffin 1:50-1:100, Western Blot 1:500 - 1:1000
MW:	43.1 kDa
Unit Size:	0.05 mg
Concentration:	1.0 mg/ml
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Buffer:	phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative:	0.02% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: MAPK3

Entrez:	5595 (Human)
	26417 (Mouse)
	50689 (Rat)
Uniprot:	P27361 (Human)

Related Genes:

Background:

p44 MAP kinase aka ERK1 is ubiquitously expressed in various tissues and cells. It is activated by phosphorylation of neighbouring serine and threonine residues by MEK1 and MEK2 in response to diverse stimuli such as growth factors and mitogens.

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ERK1/2 Antibody Images (2)

	Western Blot: p44/42 MAP Kinase Phosphospecific Antibody [NB100-82100] - Western blot analysis of extracts from NIH-3T3 cells, using p44/42 MAP Kinase (Ab-204) antibody ( Line 1 and 2) and p44/42 MAP Kinase (phospho- Tyr204) antibody (Line 3 and 4).

	Immunohistochemistry: p44/42 MAP Kinase Phosphospecific Antibody [NB100-82100] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p44/42 MAP Kinase (phospho-Tyr204) antibody

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Publications (1)

ERK1/2 Antibody Background References:

TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397 Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162 Jingui Yu, et al. (2005) Anesth Analg 101: 315-321 Hironobu Ihn et al.(2000) Immunology 165: 2149-2155