Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (E337) Details:
Clone:
E337
Isotype:
IgG
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Thr202 of human Erk1 was used as immunogen.
Modification:
p Thr202, p Tyr204, p Thr185, p Tyr187
Species Reactivity:
Reacts in Human. Does not react to Mouse or Rat.
Applications:
Uses:
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry. In Western blot this antibody detects a band at approximately 42/44kDa.
Dilutions:
Western Blot 1:1000-10000, Immunohistochemistry-Paraffin 1:50, Immunocytochemistry 1:250
TMW:
43 kDa
Unit Size:
0.1 ml
Concentration:
Please see the vial label for concentration.
Packaging:
Storage:
Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:
50 mM Tris-Glycine [pH 7.4] containing 0.15 M NaCl, 40% Glycerol and 0.05% BSA
Preservative:
0.01% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Extra cellular signal-regulated kinases Erk1 (p44 MAPK) and Erk2 (p42 MAPK) Serine/Threonine proteine kinase are part of a subfamily of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth, differentiation, apoptosis and signal transduction (1,2,3) The simultaneous tyrosine/threonine phosphorylation of ERK1 and ERK2 results in their activation. Although ERK1and ERK2 resemble one another closely in sequence and are activated by similar pathways, they appearto have different tissue distributions(4) and show differential phosphorylation of potential intracellular substrates(5)
ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (E337) Images (2)
Western Blot: ERK1/ERK2 phosphospecific Antibody (E337) [NB110-56969] - Western blot analysis on serum starved (ss)A431 cell lysate using anti-ERK1(pT202/pY204) /ERK2(pT185/pY187) (cat. #110-56969); dilution 1:500
Immunohistochemistry: ERK1/ERK2 phosphospecific Antibody (E337) [NB110-56969] - Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti- ERK1(pT202/pY204) /ERK2(pT185/pY187)