EGF Protein (NBP1-43150)
EGF Protein
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Distributor Data...
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HOMG4, anti-Epidermal growth factor antibody, anti-Beta urogastrone, URG, anti-Urogastrone, anti-URG, anti-Pro epidermal growth factor, anti-Epidermal Growth Factor, |
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EGF Protein Summary: | | | | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional EGF Products EGF Protein Details: | Immunogen: | This product is E. Coli. |
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This protein was made to the mouse sequence. |
| Uses: | This reagent has been tested by bioassays using the EGF- responsive mouse BALB/c 3T3 cell line. The ED50, as determined by the dose-dependent stimulation of 3T3 cells proliferation, is 20 pg/ml, corresponding to a specific activity of 5X10^7 units/mg. |
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| Dilutions: | Immunoassay |
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| TMW: | 6 kDa |
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| Unit Size: | 0.025 mg (also available: 0.005 mg) |
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| Concentration: | Please see the vial label for concentration. |
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Notes: Purity: Greater than 97%, as determined by SDS-PAGE Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Bioactivity: Measured by mouse BALB/c 3T3 cell proliferation assay. The ED50 is typically 20 pg/ml, corresponding to a specific activity of 5X107 units/mg. Packaging: | Storage: | Store at -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | phosphate buffered saline, 1.0% BSA. |
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| Preservative: | 0.09% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Epidermal growth factor (EGF) is the member of the EGF family that shares a structural motif, the EGF-like domain, and are synthesized as type I transmembrane precursor proteins that contain several EGF domains in the extracellular region. The mouse EGF precursor has 1217 amino acid residues containing nine EGF domains. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region resulting in a molecular mass of 6 kDa. EGF is produced by many cell types and in blood and various body fluids, including milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. EGF can promote proliferation and differentiation of mesenchymal and epithelial cells. EGF is also a mitogen for fibroblasts, epithelial and endothelial cells, and promotes colony formation of epiderma.
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Working with EGF
EGF Protein Images (1) ELISA: EGF Protein [NBP1-43150] - Standard curve of Mouse EGF Recombinant Protein. |
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