EGF Lysate (NBL1-10149)

EGF Lysate

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EGF Lysate Summary:
Applications:WB
Gene:EGF
Purity:Protein
Specificity:Homo sapiens epidermal growth factor (beta-urogastrone) (EGF), mRNA.
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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EGF Lysate Details:
Immunogen:The lysate was created in HEK293T cells, using plasmid ID RC210817 and based on accession number NM_001963. The protein contains a C-terminal DDK tag.
Lysate Details:
Type: OverexpressionLife Stage: Adult
Protein State: Native Tissue Condition: Normal
Sub-cellular Fraction: Whole
Applications:
Uses:This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Dilutions:Western Blot
TMW:131.6 kDa
Unit Size:0.1 mg
Concentration:1.0 mg/ml
Notes:
This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes. Please note that “1 mg/ml” refers to the total protein concentration and that the specific protein concentration is not determined at this time.
Packaging:
Storage:Store at -80 °C. Avoid freeze-thaw cycles.
Buffer:RIPA buffer
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: EGF
Entrez1950 (Human)
Swiss ProtNM_001963 (Human)
 
Background:

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

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Working with EGF

EGF Lysate Images (1)
Western Blot: EGF Lysate [NBL1-10149] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for EGF
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EGF in Context


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