Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide corresponing to the residues surrounding Arginine 709 of human EGF
Species Reactivity:
Human, Mouse, Rat
Applications:
Uses:
This antibody is useful for Immunohistochemistry. Immunohistochemistry analyzes of EGF (R709) antibody in paraffin-embedded human brain tissue. Purity is > 95% (by SDS-PAGE).
Dilutions:
Immunohistochemistry-Paraffin 1:50 - 1:200, Western Blot 1:500 - 1:1000
TMW:
134 kDa
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
PBS, approx. pH 7.2.
Preservative:
0.05% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid
protein of 6 kDa originally found in rodents and humans. EGF has been
shown to be a potent mitogen for a variety of cell types both in vivo and in
vitro. It binds to the 170 kDa EGF receptor on the surface of cells and mediates
intrinsic phos phoryl ation of the receptor on tyrosine residues. EGF has
been detected in nearly all body fluids such as urine (urogastrone), saliva, milk
and platelet-rich plasma. EGF, TGFalpha and vaccinia virus growth factor exhibit
30-40% amino acid homology. Several additional members of the
EGF/TGF family have been described; these include Cripto, Amphiregulin and
the heparin-binding EGF-like growth factor, each of which, except Cripto,
binds to the EGF receptor.