Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
This antibody is specific for the C Terminus Region of the target protein.
Species Reactivity:
Human.
Applications:
Uses:
If you are interested in testing this antibody in an untested application, then please see our Innovators Reward Program: https://www.novusbio.com/support/customer-service/novus-guarantee-plus.html Typically concentration ranges from 0.5 to 1.5 mg/ml.
Dilutions:
ELISA, Immunohistochemistry-Paraffin 1:250-1:2000
Unit Size:
0.05 mg
Concentration:
Please see the vial label for concentration.
Notes:
Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.
Packaging:
Storage:
Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:
20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Preservative:
No Preservative
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Dot Blot: EGF Antibody [26610002] - The anti-EGF antibody (left quadruplicate spots at each panel) or control antibody (right quadruplicate spots at each panel) were printed on membranes at 10 ng/spot. The membranes were incubated with biotinylated cell lysate (50 ug per membrane), followed by probing with streptavidin-HRP and CCD camera image acquisition. (a), Cell lysate from Jurkat cells; (b), Cell lysate from 293T cells. Data courtesy of a SDI customer.
Dot Blot: EGF Antibody [26610002] - Protein samples (left spot: 293T cell lysate; right spot: Jurkat cell lysate) were printed on membranes at 40 ng/spot. The membrane was incubated with anti-EGF antibody (1:4000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Data courtesy of a SDI customer.