E2F4 Antibody (NBP1-21374)
E2F4 Antibody
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Distributor Data...
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anti-E2F4 antibody, anti-E2F-4 antibody, anti-E2F transcription factor 4 antibody, |
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E2F4 Antibody Summary: | | Gene: | E2F4 |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional E2F4 Products E2F4 Antibody Details: | Isotype: | IgG |
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| Immunogen: | The epitope recognized by this antibody maps to a region between residue 363 and 413 of human E2F transcription factor 4 using the numbering given in entry NP_001941.2 (GeneID 1874). |
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| Uses: | This antibody is useful for Immunohistochemistry-Paraffin, Western Blot and Immunoprecipitation. |
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| Dilutions: | Immunohistochemistry-Paraffin, Immunoprecipitation 5 to 10 mcg/mg lysate, Western Blot 1:2000 to 1:10000 |
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| Unit Size: | 0.1 mg |
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| Concentration: | 1.0 mg/ml |
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Notes: Western Blot Image Notes: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Affinity purified rabbit anti-E2F4 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. E2F4 was also immunoprecipitated by rabbit anti-E2F4 antibody NBP1-21373, which recognizes an upstream epitope. Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Tris-citrate/phosphate buffer, pH 7 to 8 |
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| Preservative: | 0.09% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: E2F4 is a member of the E2F family of transcription factors. It binds DNA at the E2 recognition site found in the promoter region of a number of genes involved in cell cycle regulation and DNA replication. E2F4 binds to pRb, p107, and p130. Via its interaction with p130, E2F4 plays an important role in maintaining G2 arrest during G2 phase checkpoint activation.
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Images (2) Reviews (0) Related Products (15) Exploring E2F4
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Working with E2F4
E2F4 Antibody Images (2) Immunohistochemistry-Paraffin: E2F4 Antibody [NBP1-21374] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-E2F4 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit |  Western Blot and Immunoprecipitation: E2F4 Antibody [NBP1-21374]-Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. E2F4 was also immunoprecipitated by rabbit anti-E2F4 antibody NBP1-21373. |
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Jump to: Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsLysates (3)Peptides and ProteinsPrimary Antibodies (10)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| E2F4 Antibody | NBP1-67548 | Hu, Mu, Rt | ELISA, IHC, WB | (2) | | | | | E2F4 Antibody (SPM179) | NB120-17845 | Hu, Rt | IF, IHC-P, WB | (1) | | | | | E2F4 Antibody | NBP1-21373 | Hu | IP | (1) | | | | | E2F4 Antibody (TFE42) | NBP1-54516 | Hu, Mu, Rt | IP, WB | | | | | | E2F4 Antibody | H00001874-D01P | Hu | ELISA, WB | (2) | | | | | ... and 5 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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