Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The epitope recognized by this antibody maps to a region between residue 300 and 350 of human E2F transcription factor 4 using the numbering given in entry NP_001941.2 (GeneID 1874).
Species Reactivity:
Human
Applications:
Uses:
This antibody is useful for immunoprecipitation. It is not recommended for western analysis.
Dilutions:
Immunoprecipitation 5 to 10 mcg/mg lysate,
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
Tris-citrate/phosphate buffer, pH 7 to 8
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
E2F4 is a member of the E2F family of transcription factors. It binds DNA at the E2 recognition site found in the promoter region of a number of genes involved in cell cycle regulation and DNA replication. E2F4 binds to pRb, p107, and p130. Via its interaction with p130, E2F4 plays an important role in maintaining G2 arrest during G2 phase checkpoint activation.
Western Blot : E2F4 Antibody[NBP1-21373] - Whole cell lysate from HeLa cells.Affinity purified rabbit anti-E2F4 antibody used for IP at 10 mcg/mg lysate.