Dnmt3a Antibody (64B1446) 0.1 mg

Dnmt3a NB120-13888

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Ordering Information: NB120-13888

Catalog NumberNB120-13888
Also Known As   Imgenex: IMG-268A
  • FormUnconjugated
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Dnmt3a Antibody (64B1446) Summary

 
Species Human, Mouse
Tested Applications WB, ChIP, FLOW, ICC/IF, IHC-P
Clonality Monoclonal
Host Mouse
Gene DNMT3A
Purity Protein G purified
Guarantee Plus All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
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Dnmt3a Antibody (64B1446) Details

 
Clone 64B1446
Isotype IgG1 Kappa
Immunogen This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
Localization Nuclear

Species Reactivity

 
Cross-reacts with Human (weakly) and Mouse. Not yet tested in other species.
Publications Read Publications using NB120-13888 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 2 ug/ml
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes

Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24623306)

Positive Controls

2 Positive Controls

Other Available
Formats
7C Labeled NB120-13888V2
Alexa Fluor (R) 405 Labeled NB120-13888AF405
Alexa Fluor (R) 488 Labeled NB120-13888AF488
Alexa Fluor (R) 647 Labeled NB120-13888AF647
Alexa Fluor (R) 700 Labeled NB120-13888AF700
Allophycocyanin Labeled NB120-13888APC
DyLight 350 Labeled NB120-13888UV
DyLight 405 Labeled NB120-13888V
DyLight 405LS Labeled NB120-13888V3
DyLight 488 Labeled NB120-13888G
DyLight 550 Labeled NB120-13888R
DyLight 650 Labeled NB120-13888C
DyLight 680 Labeled NB120-13888FR
DyLight 755 Labeled NB120-13888IR
HRP Labeled NB120-13888H
PE Labeled NB120-13888PE
PerCP Labeled NB120-13888PCP
Learn about Custom Antibody Labeling
Publications
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NB120-13888 in the following applications:

Product PDFs

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Publications

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Protocols and FAQs

Controls & Support Products

Research Tools

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Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer PBS containing 0.05% BSA
Unit Size 0.1 mg (also available in 0.025 mg (sample size))
Concentration 0.5 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol DNMT3A
Entrez
Uniprot

Alternate Names for Dnmt3a

 
  • DNMT3A2
  • Dnmt3a
  • EC 2.1.1.37
  • DNA cytosine methyltransferase 3A2
  • DNA MTase HsaIIIA
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA methyltransferase HsaIIIA
  • M.HsaIIIA
  • DNA (cytosine-5-)-methyltransferase 3 alpha

Related Products by Gene

Background

 
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
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