DNMT3A Antibody (64B1446) 0.1 mg

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Western Blot: Dnmt3a Antibody (64B1446) [NB120-13888] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.
Immunocytochemistry/Immunofluorescence: Dnmt3a Antibody (64B1446) [NB120-13888] - Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with neuron-specific tubulin (green) throughout the development of the olfactory epithelium. (Data courtsey of A. Jane Roskams, University of British Columbia).
Immunohistochemistry-Paraffin: Dnmt3a Antibody (64B1446) [NB120-13888] - Formalin-fixed, paraffin-embedded human liver stained with Dnmt3a antibody at 5 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry: Dnmt3a Antibody (64B1446) [NB120-13888] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Ordering Information: NB120-13888

  • Catalog Number
    NB120-13888
  • Also Known As
    Imgenex: IMG-268A
  • Displayed Format
    Unconjugated
  • Other Formats
  • Size(s)
  • Availability
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DNMT3A Antibody (64B1446) Summary

Species Human, Mouse
Tested Applications WB, ChIP, FLOW, ICC/IF, IHC-P
Clonality
Monoclonal
Host
Mouse
Gene
DNMT3A
Purity
Protein G purified
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DNMT3A Antibody (64B1446) Details

Clone
64B1446
Isotype
IgG1 Kappa
Immunogen
This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
Localization
Nuclear

Species Reactivity

Cross-reacts with Human (weakly) and Mouse. Not yet tested in other species.
Publications Read Publications using NB120-13888 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24623306)
Positive Controls
Other Available
Formats
7C Labeled NB120-13888V2
Alexa Fluor (R) 405 Labeled NB120-13888AF405
Alexa Fluor (R) 488 Labeled NB120-13888AF488
Alexa Fluor (R) 647 Labeled NB120-13888AF647
Alexa Fluor (R) 700 Labeled NB120-13888AF700
Allophycocyanin Labeled NB120-13888APC
DyLight 350 Labeled NB120-13888UV
DyLight 405 Labeled NB120-13888V
DyLight 405LS Labeled NB120-13888V3
DyLight 488 Labeled NB120-13888G
DyLight 550 Labeled NB120-13888R
DyLight 650 Labeled NB120-13888C
DyLight 680 Labeled NB120-13888FR
DyLight 755 Labeled NB120-13888IR
HRP Labeled NB120-13888H
PE Labeled NB120-13888PE
PerCP Labeled NB120-13888PCP
Publications
Read Publications using
NB120-13888 in the following applications:

Contact Information

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Publications

DNMT3A Antibody (NB120-13888) has been mentioned in at least 56 publications.
Earn rewards if you have published using DNMT3A Antibody (NB120-13888).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Unit Size
0.1 mg
Concentration
0.5 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol DNMT3A
Entrez
Uniprot

Alternate Names for DNMT3A Antibody (64B1446)

  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA cytosine methyltransferase 3A2
  • DNA methyltransferase HsaIIIA
  • DNA MTase HsaIIIA
  • Dnmt3a
  • DNMT3A2
  • EC 2.1.1.37
  • M.HsaIIIA
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Related Products by Gene

Background

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
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Publications for DNMT3A Antibody (NB120-13888) (56)

Read what people are writing who have used DNMT3A Antibody (NB120-13888).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: WB, ChIP, ICC/IF, IHC-Fr, IHC-P.

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Filter By Application
WB
(18)
ChIP
(4)
ICC/IF
(3)
IHC-Fr
(1)
IHC-P
(2)
All Applications
Filter By Species
Human
(7)
Mouse
(14)
All Species
Publication Applications Species
Fan H, Liu D, Qiu X et al. A functional polymorphism in the DNA methyltransferase-3A promoter modifies the susceptibility in gastric cancer but not in esophageal carcinoma. BMC Med. 2010 Feb 3 [PMID: 20128888]
Chen T, Ueda Y, Xie S, Li E. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem. 2002 Oct 11 [PMID: 12138111]
Park IY, Sohn BH, Yu E et al. Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein. Gastroenterology. 2007 Apr [PMID: 17408664] (WB)

Details:
(Chang liver and Hep G2 cell lines): 1. IMG-261(DNMT1): WB, Figs. 1A, 4A,B. 2. IMG-268A (DNMT3A): WB, ChIP, Figs. 1B, 2B, 4A,B.
WB
Shen Y, Chow J, Wang Z, Fan G. Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cells. Hum Mol Genet. 2006 Sep 1 [PMID: 16870691] (ICC/IF) ICC/IF
Zimmermann N, Zschocke J, Perisic T et al. Antidepressants inhibit DNA methyltransferase 1 through reducing G9a levels. Biochem J. 2012 Nov 15 [PMID: 22880885]
Stadtfeld M, Apostolou E, Ferrari F et al. Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells. Nat Genet. 2012 Mar 4 [PMID: 22387999]
Yan Q, Cho E, Lockett S, Muegge K. Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin. Mol Cell Biol. 2003 Dec [PMID: 14612388]
Wu Y, Alvarez M, Slamon DJ et al. Caspase 8 and maspin are downregulated in breast cancer cells due to CpG site promoter methylation. BMC Cancer. 2010 Feb 4 [PMID: 20132554]
Stempak JM, Sohn KJ, Chiang EP et al. Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model. Carcinogenesis. 2005 May [PMID: 15695236]
Lucifero D, La Salle S, Bourc'his D et al. Coordinate regulation of DNA methyltransferase expression during oogenesis. BMC Dev Biol. 2007 Apr 19 [PMID: 17445268] (WB, Mouse)

Details:
Products cited for WB (growing mouse oocytes and type B spermatogonia): 1. DNMT3a (IMG-268A), Fig 5a, b (middle panel). The antibody detected both the DNMT3a (908 aa) and DNMT3a2 (689 aa) isoforms. 2. DNMT3b (IMG-184A), Fig 5c (top panel). The antibody de
WB Mouse
Pawlak M, Jaenisch R. De novo DNA methylation by Dnmt3a and Dnmt3b is dispensable for nuclear reprogramming of somatic cells to a pluripotent state. Genes Dev. 2011 May 15 [PMID: 21576263]
Oka M, Meacham AM, Hamazaki T et al. De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine. Oncogene. 2005 Apr 28 [PMID: 15735669]
Park IY, Sohn BH, Choo JH et al. Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development. J Cell Biochem. 2005 Feb 15 [PMID: 15543560]
Iwatani M, Ikegami K, Kremenska Y et al. Dimethyl sulfoxide has an impact on epigenetic profile in mouse embryoid body. Stem Cells. 2006 Nov [PMID: 16840553]
Nasonkin IO, Lazo K, Hambright D et al. Distinct nuclear localization patterns of DNA methyltransferases in developing and mature mammalian retina. J Comp Neurol. 2011 Jul 1 [PMID: 21452232]
Kashiwagi K, Nimura K, Ura K, Kaneda Y. DNA methyltransferase 3b preferentially associates with condensed chromatin. Nucleic Acids Res. 2011 Feb [PMID: 20923784]
Kadriu B, Guidotti A, Chen Y, Grayson DR. DNA methyltransferases1 (DNMT1) and 3a (DNMT3a) colocalize with GAD67-positive neurons in the GAD67-GFP mouse brain. J Comp Neurol. 2012 Jun 15 [PMID: 22134929] (WB, IHC-Fr, Mouse)

Details:
Antibodies cited: 1. DNMT1 (IMG-261A): WB (mouse brain cortical extracts), Table 1; IHC (frozen) (mouse brain), Figs 5, 6, 8B, 8C. 2. DNMT3A (IMG-268A): WB (mouse brain cortical extracts), Table 1; IHC (frozen) (mouse brain), Fig 7. Note: The specificitie
WB, IHC-Fr Mouse
Nimura K, Ishida C, Koriyama H et al. Dnmt3a2 targets endogenous Dnmt3L to ES cell chromatin and induces regional DNA methylation. Genes Cells. 2006 Oct [PMID: 16999741]
Linhart HG, Lin H, Yamada Y et al. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev. 2007 Dec 1 [PMID: 18056424] (WB, Mouse)

Details:
Products cited (mouse tumor cells): 1. IMG-184A (Dnmt3b): IHC (paraffin); Figs. 1C, 2A. Dnmt3b transgene validated in mice in Figs. 1C, 2A. 2. IMG-268A (Dnmt3a): IHC (paraffin), Wb; Figs. 1C, 2A,B. Dnmt3a transgene validated in mice in Figs. 1C, 2A,B.
WB Mouse
Feng J, Chang H, Li E, Fan G. Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system. J Neurosci Res. 2005 Mar 15 [PMID: 15672446]
La Salle S, Trasler JM. Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse. Dev Biol. 2006 Aug 1 [PMID: 16725135] (WB, Mouse)

Details:
Products cited for WB (primary male mouse germ cells and testes): 1. DNMT3a (IMG-268A), Fig 5B (middle panel). The antibody detected both the DNMT3a (908 aa) and DNMT3a2 (689 aa) isoforms. 2. DNMT3b (IMG-184A), Fig 5C (top panel). The antibody detected mu
WB Mouse
Sheikh MA, Malik YS, Yu H et al. Epigenetic regulation of Dpp6 expression by Dnmt3b and its novel role in the inhibition of RA induced neuronal differentiation of P19 cells. PLoS One. 2013 [PMID: 23409053]
Ko CY, Hsu HC, Shen MR et al. Epigenetic silencing of CCAAT/enhancer-binding protein delta activity by YY1/polycomb group/DNA methyltransferase complex. J Biol Chem. 2008 Nov 7 [PMID: 18753137] (WB)

Details:
HeLa cells in Fig. 7A (IF), 7B (WB following a DNA Affinity Precipitation Assay of nuclear extracts), 7C and D (ChIP): 1. IMG-261A (Dnmt1) 2. IMG-268A (Dnmt3a) 3. IMG-184A (Dnmt3b).
WB
Chen T, Ueda Y, Dodge JE et al. Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Mol Cell Biol. 2003 Aug [PMID: 12897133] (WB, Mouse)

Details:
WB [mouse embryonic stem (ES) cell lines and ES cells expressing various Dnmt1, 3a and 3b isoforms], Figs 1B and 7A.
WB Mouse
Choi MS, Shim YH, Hwa JY et al. Expression of DNA methyltransferases in multistep hepatocarcinogenesis. Hum Pathol. 2003 Jan [PMID: 12605361]
Galetzka D, Tralau T, Stein R, Haaf T. Expression of DNMT3A transcripts and nucleolar localization of DNMT3A protein in human testicular and fibroblast cells suggest a role for de novo DNA methylation in nucleolar inactivation. J Cell Biochem. 2006 Jul 1 [PMID: 16453278]
Xia G, Schneider-Stock R, Diestel A et al. Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation. Biochem Biophys Res Commun. 2008 May 2 [PMID: 18302936] (WB, Human)

Details:
(human NCI N87 gastric carcinoma cells): WB (Fig 1) 1. Dnmt1 (IMG-261). 2. Dnmt3a (IMG-268) 3. Dnmt3b (IMG-184).
WB Human
Park ES, Park YK, Shin CY et al. Hepatitis B virus inhibits liver regeneration via epigenetic regulation of urokinase-type plasminogen activator. Hepatology. 2013 Aug [PMID: 23483589]
Qiu YY, Mirkin BL, Dwivedi RS. Inhibition of DNA methyltransferase reverses cisplatin induced drug resistance in murine neuroblastoma cells. Cancer Detect Prev. 2005 [PMID: 16185816]
Jones B, Chen J. Inhibition of IFN-gamma transcription by site-specific methylation during T helper cell development. EMBO J. 2006 Jun 7 [PMID: 16724115] (ChIP) ChIP
Dennis K, Fan T, Geiman T et al. Lsh, a member of the SNF2 family, is required for genome-wide methylation. Genes Dev. 2001 Nov 15 [PMID: 11711429]
Tsumura A, Hayakawa T, Kumaki Y et al. Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Genes Cells. 2006 Jul [PMID: 16824199]
Hirasawa R, Chiba H, Kaneda M et al. Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. Genes Dev. 2008 Jun 15 [PMID: 18559477] (IF/ICC, Mouse)

Details:
Antibodies cited: 1. Dnmt3a (IMG-268a): IF/ICC (mouse oocytes and embryos), Fig. 1a. 2. Dnmt3b (IMG-184): IF/ICC (mouse occytes and embryos), Fig. 1c.
Mouse
May A, Kirchner R, Muller H et al. Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres. Biol Reprod. 2009 Jan [PMID: 18784354] (IF/ICC, Mouse)

Details:
IF/ICC (mouse blastomeres), Fig. 5A.
Mouse
Leitch HG, McEwen KR, Turp A et al. Naive pluripotency is associated with global DNA hypomethylation. Nat Struct Mol Biol. 2013 Mar [PMID: 23416945]
Meilinger D, Fellinger K, Bultmann S et al. Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells. EMBO Rep. 2009 Nov [PMID: 19798101]
Selvakumar T, Gjidoda A, Hovde SL, Henry RW. Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases. J Biol Chem. 2012 Mar 2 [PMID: 22219193]
Weisenberger DJ, Velicescu M, Cheng JC et al. Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation. Mol Cancer Res. 2004 Jan [PMID: 14757847] (WB)

Details:
Products cited for WB [wild-type mouse embryonic stem (ES) cells and ES knockouts of Dnmt3a, Dnmt3b, and 3a/b]: 1. DNMT3a (IMG-268A), Fig 2A, B. The specificity of the antibody is knockout mouse validated as it did not detect Dnmt3a in either the 3a-/- (M
WB
Ueda Y, Okano M, Williams C et al. Roles for Dnmt3b in mammalian development: a mouse model for the ICF syndrome. Development. 2006 Mar [PMID: 16501171]
Hong GM, Bain LJ. Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1. Toxicol Appl Pharmacol. 2012 May 1 [PMID: 22426358]
MacDonald JL, Gin CS, Roskams AJ. Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development. Dev Biol. 2005 Dec 15 [PMID: 16288735] (IHC-P) IHC-P
Hara ES1, Ono M1, Eguchi T2 et al. miRNA-720 Controls Stem Cell Phenotype, Proliferation and Differentiation of Human Dental Pulp Cells. PLoS One 2013 Dec 30 [PMID: 24386225] (ICC/IF, Human) ICC/IF Human
Wongtrakoongate P, Li J, Andrews PW. DNMT3B inhibits the re-expression of genes associated with induced pluripotency. Exp Cell Res 2013 Dec 10 [PMID: 24333507] (WB, Human) WB Human
Zhou JX, Dhawan S, Fu H et al. Combined modulation of polycomb and trithorax genes rejuvenates beta cell replication. J Clin Invest 2013 Oct 8 [PMID: 24216481] (WB, Mouse) WB Mouse
Hara S, Takano T, Fujikawa T et al. Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting. Hum. Mol. Genet. 2014 Mar 18 [PMID: 24599402] (WB, Mouse)

Details:
Mouse WT oocytes and oocytes DNMT3A2 transgenic mice, Figs 1, 2D-E, 4A. The mAb detected both 3A (130 kDa) and the smaller 3A2 (85 kDa) isoforms. DNMT3A2 is a short DNMT3A isoform produced from an alternative promoter. Note: The specificity of the antibod
WB Mouse
Hara S, Takano T, Ogata M et al. Establishment of a Conditional Transgenic System Using the 2A Peptide in the Female Mouse Germline. J. Reprod. Dev. 2014 Mar 15 [PMID: 24632680] (WB, Mouse)

Details:
DNMT3A2-transgenic mouse models amd DNMT3A2-transfected cells (Figs 1C, 2E, F). DNMT3A2 was detected at approx. 85 kDa (Fig 1C), DNMT3A1 was detected at approx. 135kDa (Fig 2E). The specificity of the DNMT3A mAb was validated in DNMT3A2 transgenic mouse m
WB Mouse
Obata Y, Wakai T, Hara S, Kono T et al. Long exposure to mature ooplasm can alter DNA methylation at imprinted loci in non-growing oocytes but not in prospermatogonia. Reproduction. 2014 Jan 1 [PMID: 24123131] (ICC/IF, Mouse)

Details:
Oocyte/prospermatogonium fusions, Fig 3.
ICC/IF Mouse
Serra FW, Fang M, Park SM et al. A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. Elife 2014 [PMID: 24623306] (ChIP, Human) ChIP Human
Paredes-Gonzalez X, Fuentes F, Su ZY, Kong AN. Apigenin Reactivates Nrf2 Anti-oxidative Stress Signaling in Mouse Skin Epidermal JB6 P + Cells Through Epigenetics Modifications. AAPS J. 2014 May 16 [PMID: 24830944] (WB, Mouse)

Details:
JB6 P+ mouse skin epidermal cells, Fig 6a.
WB Mouse
Leu YW, Yan PS, Fan M et al. Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer. Cancer Res. 2004 Nov 15 [PMID: 15548683] (ChIP) ChIP
Guimaraes DM, Antunes DM, Duarte CM et al. DNA methyltransferase immunohistochemical expression in odontogenic tumours J. Oral Pathol. Med. 2014 Jul 24 [PMID: 25060239] (IHC-P, Human)

Details:
Dnmt3a antibody used for IHC-P at 1:200 dilution on formalin-fixed and paraffin-embedded tissue sections of human ameloblastomas, calcifying cystic odontogenic tumours, calcifying epithelial tumours, adenomatoid odontogenic tumours, keratocystic odontogenic tumours, ameloblastic fibromas, ameloblastic fibro-odontomas, central odontogenic fibromas, peripheral odontogenic fibromas and odontogenic myxomas.
IHC-P Human
Emperle M, Rajavelu A, Reinhardt R et al. Cooperative DNA binding and protein/DNA fiber formation increases the activity of the Dnmt3a DNA methyltransferase J. Biol. Chem. 2014 Aug 21 [PMID: 25147181] (WB) WB
Wang L, Zhang C, Guo Y et al. Blocking of JB6 Cell Transformation by Tanshinone IIA: Epigenetic Reactivation of Nrf2 Antioxidative Stress Pathway. AAPS J. 2014 Nov 01 [PMID: 25274607] (WB, Mouse)

Details:
DNMT3a antibody used for WB on lysates of JB6 P+ cells (a mouse keratinocyte cell line) treated or not with Tanshinone IIA (TIIA), an active component in Salvia miltiorrhiza that has been reported to activate Nrf2 pathway.
WB Mouse
Huichalaf C, Micheloni S, Ferri G et al. DNA Methylation Analysis of the Macrosatellite Repeat Associated with FSHD Muscular Dystrophy at Single Nucleotide Level. PLoS ONE. 2014 Dec 30 [PMID: 25545674] (ChIP, Human)

Details:
DNMT3A antibody used for ChIP in experiments involving human chromosome 4/CHO (Chr4/CHO) hybrid cells (a human-hamster hybrid cell line).
ChIP Human
Guo Y, Shu L, Zhang C et al. Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1 Biochem. Pharmacol. 2015 Jan 29 [PMID: 25640947] (WB, Human)

Details:
DNMT3A antibody used for WB on lysates of HT29 human colon cancer cells treated or not with 2.5uM or 5uM Curcumin, and 2.5uM azadeoxycytidine/5AZA + 100uM trichostatin A/TSA. Curcumin was found to downregualte the expression levels of DNMT3A protein in a concentration-dependent manner (Fig 5).
WB Human
Neri F, Incarnato D, Krepelova A et al. Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics Cell Rep. 2015 Feb 04 [PMID: 25660018] (WB, Mouse)

Details:
DNMT3A antibody (clone 64B1446) used for WB analysis of lysates from mouse embryonic stem cell / mESCs which were transfected with either control shRNA (shCtr) or shRNAs targeting Dnmts namely Sh-Dnmt1, Sh-Dnmt3a, Sh-Dnmt3b and Sh-Dnmt3a/b (Figure 4E).
WB Mouse

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FAQs for DNMT3A (3)

  1. Do you have any information about the epitope of your antibody, so I could check the chances for the antibody working in the honey bee?
    • This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details. We haven't mapped the binding binding epitope although Chen et (2002) mapped it to a range between 705-908 amino acids: I am not sure what technique you are planning with the antibody, but if you are doing a western blot, we do have a western blot postive control ready to use lysate NBP2-25063 and I would l highly suggest using that.
  2. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  3. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Bioinformatics Tool for DNMT3A

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Blogs on DNMT3A

Check out the latest blog posts on DNMT3A.
Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.