DNMT3A Antibody (64B1446) 0.1 mg

Click image to view larger
Western Blot: Dnmt3a Antibody (64B1446) [NB120-13888] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.
Immunocytochemistry/Immunofluorescence: Dnmt3a Antibody (64B1446) [NB120-13888] - Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with neuron-specific tubulin (green) throughout the development of the olfactory epithelium. (Data courtsey of A. Jane Roskams, University of British Columbia).
Immunohistochemistry-Paraffin: Dnmt3a Antibody (64B1446) [NB120-13888] - Formalin-fixed, paraffin-embedded human liver stained with Dnmt3a antibody at 5 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry: Dnmt3a Antibody (64B1446) [NB120-13888] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Download Datasheet
See All Related

Ordering Information: NB120-13888

  • Catalog Number
    NB120-13888
  • Also Known As
    Imgenex: IMG-268A
  • Displayed Format
    Unconjugated
  • Other Formats
  • Size(s)
  • Availability
  • Price
    Shipping
  

DNMT3A Antibody (64B1446) Summary

Species Human, Mouse
Tested Applications WB, ChIP, FLOW, ICC/IF, IHC-P
Clonality
Monoclonal
Host
Mouse
Gene
DNMT3A
Purity
Protein G purified
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

DNMT3A Antibody (64B1446) Details

Clone
64B1446
Isotype
IgG1 Kappa
Immunogen
This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
Localization
Nuclear

Species Reactivity

Cross-reacts with Human (weakly) and Mouse. Not yet tested in other species.
Publications Read Publications using NB120-13888 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24623306)
Positive Controls
Other Available
Formats
7C Labeled NB120-13888V2
Alexa Fluor (R) 405 Labeled NB120-13888AF405
Alexa Fluor (R) 488 Labeled NB120-13888AF488
Alexa Fluor (R) 647 Labeled NB120-13888AF647
Alexa Fluor (R) 700 Labeled NB120-13888AF700
Allophycocyanin Labeled NB120-13888APC
DyLight 350 Labeled NB120-13888UV
DyLight 405 Labeled NB120-13888V
DyLight 405LS Labeled NB120-13888V3
DyLight 488 Labeled NB120-13888G
DyLight 550 Labeled NB120-13888R
DyLight 650 Labeled NB120-13888C
DyLight 680 Labeled NB120-13888FR
DyLight 755 Labeled NB120-13888IR
HRP Labeled NB120-13888H
PE Labeled NB120-13888PE
PerCP Labeled NB120-13888PCP
Publications
Read Publications using
NB120-13888 in the following applications:

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Earn rewards points by submitting your review for DNMT3A Antibody (NB120-13888). Be the first to review this product and earn double rewards points!

Publications

DNMT3A Antibody (NB120-13888) has been mentioned in at least 52 publications.
Earn rewards if you have published using DNMT3A Antibody (NB120-13888).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Unit Size
0.1 mg
Concentration
0.5 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol DNMT3A
Entrez
Uniprot

Alternate Names for DNMT3A Antibody (64B1446)

  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA cytosine methyltransferase 3A2
  • DNA methyltransferase HsaIIIA
  • DNA MTase HsaIIIA
  • Dnmt3a
  • DNMT3A2
  • EC 2.1.1.37
  • M.HsaIIIA
Show More

Related Products by Gene

Background

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
Supplier Logo

Reviews for DNMT3A Antibody (NB120-13888) (0)

There are no reviews for DNMT3A Antibody (NB120-13888).
By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image
Have you used DNMT3A Antibody (NB120-13888)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Publications for DNMT3A Antibody (NB120-13888) (52)

Read what people are writing who have used DNMT3A Antibody (NB120-13888).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: WB, ChIP, ICC/IF, IHC-Fr, IHC-P.

Have you published using DNMT3A Antibody (NB120-13888)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(15)
ChIP
(3)
ICC/IF
(3)
IHC-Fr
(1)
IHC-P
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(12)
All Species
Publication Applications Species
Fan H, Liu D, Qiu X et al. A functional polymorphism in the DNA methyltransferase-3A promoter modifies the susceptibility in gastric cancer but not in esophageal carcinoma. BMC Med. 2010 Feb 3 [PMID: 20128888]
Chen T, Ueda Y, Xie S, Li E. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem. 2002 Oct 11 [PMID: 12138111]
Park IY, Sohn BH, Yu E et al. Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein. Gastroenterology. 2007 Apr [PMID: 17408664] (WB)

Details:
(Chang liver and Hep G2 cell lines): 1. IMG-261(DNMT1): WB, Figs. 1A, 4A,B. 2. IMG-268A (DNMT3A): WB, ChIP, Figs. 1B, 2B, 4A,B.
WB
Shen Y, Chow J, Wang Z, Fan G. Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cells. Hum Mol Genet. 2006 Sep 1 [PMID: 16870691] (ICC/IF) ICC/IF
Zimmermann N, Zschocke J, Perisic T et al. Antidepressants inhibit DNA methyltransferase 1 through reducing G9a levels. Biochem J. 2012 Nov 15 [PMID: 22880885]
Stadtfeld M, Apostolou E, Ferrari F et al. Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells. Nat Genet. 2012 Mar 4 [PMID: 22387999]
Yan Q, Cho E, Lockett S, Muegge K. Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin. Mol Cell Biol. 2003 Dec [PMID: 14612388]
Wu Y, Alvarez M, Slamon DJ et al. Caspase 8 and maspin are downregulated in breast cancer cells due to CpG site promoter methylation. BMC Cancer. 2010 Feb 4 [PMID: 20132554]
Stempak JM, Sohn KJ, Chiang EP et al. Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model. Carcinogenesis. 2005 May [PMID: 15695236]
Lucifero D, La Salle S, Bourc'his D et al. Coordinate regulation of DNA methyltransferase expression during oogenesis. BMC Dev Biol. 2007 Apr 19 [PMID: 17445268] (WB, Mouse)

Details:
Products cited for WB (growing mouse oocytes and type B spermatogonia): 1. DNMT3a (IMG-268A), Fig 5a, b (middle panel). The antibody detected both the DNMT3a (908 aa) and DNMT3a2 (689 aa) isoforms. 2. DNMT3b (IMG-184A), Fig 5c (top panel). The antibody de
WB Mouse
Pawlak M, Jaenisch R. De novo DNA methylation by Dnmt3a and Dnmt3b is dispensable for nuclear reprogramming of somatic cells to a pluripotent state. Genes Dev. 2011 May 15 [PMID: 21576263]
Oka M, Meacham AM, Hamazaki T et al. De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine. Oncogene. 2005 Apr 28 [PMID: 15735669]
Park IY, Sohn BH, Choo JH et al. Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development. J Cell Biochem. 2005 Feb 15 [PMID: 15543560]
Iwatani M, Ikegami K, Kremenska Y et al. Dimethyl sulfoxide has an impact on epigenetic profile in mouse embryoid body. Stem Cells. 2006 Nov [PMID: 16840553]
Nasonkin IO, Lazo K, Hambright D et al. Distinct nuclear localization patterns of DNA methyltransferases in developing and mature mammalian retina. J Comp Neurol. 2011 Jul 1 [PMID: 21452232]
Kashiwagi K, Nimura K, Ura K, Kaneda Y. DNA methyltransferase 3b preferentially associates with condensed chromatin. Nucleic Acids Res. 2011 Feb [PMID: 20923784]
Kadriu B, Guidotti A, Chen Y, Grayson DR. DNA methyltransferases1 (DNMT1) and 3a (DNMT3a) colocalize with GAD67-positive neurons in the GAD67-GFP mouse brain. J Comp Neurol. 2012 Jun 15 [PMID: 22134929] (WB, IHC-Fr, Mouse)

Details:
Antibodies cited: 1. DNMT1 (IMG-261A): WB (mouse brain cortical extracts), Table 1; IHC (frozen) (mouse brain), Figs 5, 6, 8B, 8C. 2. DNMT3A (IMG-268A): WB (mouse brain cortical extracts), Table 1; IHC (frozen) (mouse brain), Fig 7. Note: The specificitie
WB, IHC-Fr Mouse
Nimura K, Ishida C, Koriyama H et al. Dnmt3a2 targets endogenous Dnmt3L to ES cell chromatin and induces regional DNA methylation. Genes Cells. 2006 Oct [PMID: 16999741]
Linhart HG, Lin H, Yamada Y et al. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev. 2007 Dec 1 [PMID: 18056424] (WB, Mouse)

Details:
Products cited (mouse tumor cells): 1. IMG-184A (Dnmt3b): IHC (paraffin); Figs. 1C, 2A. Dnmt3b transgene validated in mice in Figs. 1C, 2A. 2. IMG-268A (Dnmt3a): IHC (paraffin), Wb; Figs. 1C, 2A,B. Dnmt3a transgene validated in mice in Figs. 1C, 2A,B.
WB Mouse
Feng J, Chang H, Li E, Fan G. Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system. J Neurosci Res. 2005 Mar 15 [PMID: 15672446]
La Salle S, Trasler JM. Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse. Dev Biol. 2006 Aug 1 [PMID: 16725135] (WB, Mouse)

Details:
Products cited for WB (primary male mouse germ cells and testes): 1. DNMT3a (IMG-268A), Fig 5B (middle panel). The antibody detected both the DNMT3a (908 aa) and DNMT3a2 (689 aa) isoforms. 2. DNMT3b (IMG-184A), Fig 5C (top panel). The antibody detected mu
WB Mouse
Sheikh MA, Malik YS, Yu H et al. Epigenetic regulation of Dpp6 expression by Dnmt3b and its novel role in the inhibition of RA induced neuronal differentiation of P19 cells. PLoS One. 2013 [PMID: 23409053]
Ko CY, Hsu HC, Shen MR et al. Epigenetic silencing of CCAAT/enhancer-binding protein delta activity by YY1/polycomb group/DNA methyltransferase complex. J Biol Chem. 2008 Nov 7 [PMID: 18753137] (WB)

Details:
HeLa cells in Fig. 7A (IF), 7B (WB following a DNA Affinity Precipitation Assay of nuclear extracts), 7C and D (ChIP): 1. IMG-261A (Dnmt1) 2. IMG-268A (Dnmt3a) 3. IMG-184A (Dnmt3b).
WB
Chen T, Ueda Y, Dodge JE et al. Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Mol Cell Biol. 2003 Aug [PMID: 12897133] (WB, Mouse)

Details:
WB [mouse embryonic stem (ES) cell lines and ES cells expressing various Dnmt1, 3a and 3b isoforms], Figs 1B and 7A.
WB Mouse
Choi MS, Shim YH, Hwa JY et al. Expression of DNA methyltransferases in multistep hepatocarcinogenesis. Hum Pathol. 2003 Jan [PMID: 12605361]
Galetzka D, Tralau T, Stein R, Haaf T. Expression of DNMT3A transcripts and nucleolar localization of DNMT3A protein in human testicular and fibroblast cells suggest a role for de novo DNA methylation in nucleolar inactivation. J Cell Biochem. 2006 Jul 1 [PMID: 16453278]
Xia G, Schneider-Stock R, Diestel A et al. Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation. Biochem Biophys Res Commun. 2008 May 2 [PMID: 18302936] (WB, Human)

Details:
(human NCI N87 gastric carcinoma cells): WB (Fig 1) 1. Dnmt1 (IMG-261). 2. Dnmt3a (IMG-268) 3. Dnmt3b (IMG-184).
WB Human
Park ES, Park YK, Shin CY et al. Hepatitis B virus inhibits liver regeneration via epigenetic regulation of urokinase-type plasminogen activator. Hepatology. 2013 Aug [PMID: 23483589]
Qiu YY, Mirkin BL, Dwivedi RS. Inhibition of DNA methyltransferase reverses cisplatin induced drug resistance in murine neuroblastoma cells. Cancer Detect Prev. 2005 [PMID: 16185816]
Jones B, Chen J. Inhibition of IFN-gamma transcription by site-specific methylation during T helper cell development. EMBO J. 2006 Jun 7 [PMID: 16724115] (ChIP) ChIP
Dennis K, Fan T, Geiman T et al. Lsh, a member of the SNF2 family, is required for genome-wide methylation. Genes Dev. 2001 Nov 15 [PMID: 11711429]
Tsumura A, Hayakawa T, Kumaki Y et al. Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Genes Cells. 2006 Jul [PMID: 16824199]
Hirasawa R, Chiba H, Kaneda M et al. Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. Genes Dev. 2008 Jun 15 [PMID: 18559477] (IF/ICC, Mouse)

Details:
Antibodies cited: 1. Dnmt3a (IMG-268a): IF/ICC (mouse oocytes and embryos), Fig. 1a. 2. Dnmt3b (IMG-184): IF/ICC (mouse occytes and embryos), Fig. 1c.
Mouse
May A, Kirchner R, Muller H et al. Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres. Biol Reprod. 2009 Jan [PMID: 18784354] (IF/ICC, Mouse)

Details:
IF/ICC (mouse blastomeres), Fig. 5A.
Mouse
Leitch HG, McEwen KR, Turp A et al. Naive pluripotency is associated with global DNA hypomethylation. Nat Struct Mol Biol. 2013 Mar [PMID: 23416945]
Meilinger D, Fellinger K, Bultmann S et al. Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells. EMBO Rep. 2009 Nov [PMID: 19798101]
Selvakumar T, Gjidoda A, Hovde SL, Henry RW. Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases. J Biol Chem. 2012 Mar 2 [PMID: 22219193]
Weisenberger DJ, Velicescu M, Cheng JC et al. Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation. Mol Cancer Res. 2004 Jan [PMID: 14757847] (WB)

Details:
Products cited for WB [wild-type mouse embryonic stem (ES) cells and ES knockouts of Dnmt3a, Dnmt3b, and 3a/b]: 1. DNMT3a (IMG-268A), Fig 2A, B. The specificity of the antibody is knockout mouse validated as it did not detect Dnmt3a in either the 3a-/- (M
WB
Ueda Y, Okano M, Williams C et al. Roles for Dnmt3b in mammalian development: a mouse model for the ICF syndrome. Development. 2006 Mar [PMID: 16501171]
Hong GM, Bain LJ. Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1. Toxicol Appl Pharmacol. 2012 May 1 [PMID: 22426358]
MacDonald JL, Gin CS, Roskams AJ. Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development. Dev Biol. 2005 Dec 15 [PMID: 16288735] (IHC-P) IHC-P
Hara ES1, Ono M1, Eguchi T2 et al. miRNA-720 Controls Stem Cell Phenotype, Proliferation and Differentiation of Human Dental Pulp Cells. PLoS One 2013 Dec 30 [PMID: 24386225] (ICC/IF, Human) ICC/IF Human
Wongtrakoongate P, Li J, Andrews PW. DNMT3B inhibits the re-expression of genes associated with induced pluripotency. Exp Cell Res 2013 Dec 10 [PMID: 24333507] (WB, Human) WB Human
Zhou JX, Dhawan S, Fu H et al. Combined modulation of polycomb and trithorax genes rejuvenates beta cell replication. J Clin Invest 2013 Oct 8 [PMID: 24216481] (WB, Mouse) WB Mouse
Hara S, Takano T, Fujikawa T et al. Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting. Hum. Mol. Genet. 2014 Mar 18 [PMID: 24599402] (WB, Mouse)

Details:
Mouse WT oocytes and oocytes DNMT3A2 transgenic mice, Figs 1, 2D-E, 4A. The mAb detected both 3A (130 kDa) and the smaller 3A2 (85 kDa) isoforms. DNMT3A2 is a short DNMT3A isoform produced from an alternative promoter. Note: The specificity of the antibod
WB Mouse
Hara S, Takano T, Ogata M et al. Establishment of a Conditional Transgenic System Using the 2A Peptide in the Female Mouse Germline. J. Reprod. Dev. 2014 Mar 15 [PMID: 24632680] (WB, Mouse)

Details:
DNMT3A2-transgenic mouse models amd DNMT3A2-transfected cells (Figs 1C, 2E, F). DNMT3A2 was detected at approx. 85 kDa (Fig 1C), DNMT3A1 was detected at approx. 135kDa (Fig 2E). The specificity of the DNMT3A mAb was validated in DNMT3A2 transgenic mouse m
WB Mouse
Obata Y, Wakai T, Hara S, Kono T et al. Long exposure to mature ooplasm can alter DNA methylation at imprinted loci in non-growing oocytes but not in prospermatogonia. Reproduction. 2014 Jan 1 [PMID: 24123131] (ICC/IF, Mouse)

Details:
Oocyte/prospermatogonium fusions, Fig 3.
ICC/IF Mouse
Serra FW, Fang M, Park SM et al. A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. Elife 2014 [PMID: 24623306] (ChIP, Human) ChIP Human
Paredes-Gonzalez X, Fuentes F, Su ZY, Kong AN. Apigenin Reactivates Nrf2 Anti-oxidative Stress Signaling in Mouse Skin Epidermal JB6 P + Cells Through Epigenetics Modifications. AAPS J. 2014 May 16 [PMID: 24830944] (WB, Mouse)

Details:
JB6 P+ mouse skin epidermal cells, Fig 6a.
WB Mouse
Leu YW, Yan PS, Fan M et al. Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer. Cancer Res. 2004 Nov 15 [PMID: 15548683] (ChIP) ChIP
Guimaraes DM, Antunes DM, Duarte CM et al. DNA methyltransferase immunohistochemical expression in odontogenic tumours J. Oral Pathol. Med. 2014 Jul 24 [PMID: 25060239] (IHC-P, Human)

Details:
Dnmt3a antibody used for IHC-P at 1:200 dilution on formalin-fixed and paraffin-embedded tissue sections of human ameloblastomas, calcifying cystic odontogenic tumours, calcifying epithelial tumours, adenomatoid odontogenic tumours, keratocystic odontogenic tumours, ameloblastic fibromas, ameloblastic fibro-odontomas, central odontogenic fibromas, peripheral odontogenic fibromas and odontogenic myxomas.
IHC-P Human
Emperle M, Rajavelu A, Reinhardt R et al. Cooperative DNA binding and protein/DNA fiber formation increases the activity of the Dnmt3a DNA methyltransferase J. Biol. Chem. 2014 Aug 21 [PMID: 25147181] (WB) WB

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for DNMT3A (3)

  1. Do you have any information about the epitope of your antibody, so I could check the chances for the antibody working in the honey bee?
    • This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details. We haven't mapped the binding binding epitope although Chen et (2002) mapped it to a range between 705-908 amino acids: I am not sure what technique you are planning with the antibody, but if you are doing a western blot, we do have a western blot postive control ready to use lysate NBP2-25063 and I would l highly suggest using that.
  2. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  3. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Customers Who Viewed This Item Also Viewed...

NB100-56519
Western Blot: Dnmt1 Antibody (60B1220.1) [NB100-56519] - analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. this antibody. 5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. ’s goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunocytochemistry/Immunofluorescence: Dnmt1 Antibody (60B1220.1) [NB100-56519] - Dnmt1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.Immunohistochemistry-Paraffin: Dnmt1 Antibody (60B1220.1) [NB100-56519] - analysis of Dnmt1 using antibody at 2 ug/ml on formalin-fixed, paraffin-embedded medullar kidney tissue sections

Mouse Monoclonal
Species Zebrafish, Mouse, Human
Applications IHC-FrFl, IP, IHC-P...MORE

60 Publications
NB100-56514
Western Blot: Dnmt3b Antibody (52A1018) [NB100-56514] - Analysis of Dnmt3b in A) NIH 3T3 cell lysate, B) recombinant Dnmt3b1 protein (NB100-56514), and C) 2102Ep human embryonal carcinoma cell lysate using NB100-56514 at 4 ug/ml, 0.5 ug/ml, and 3 ug/ml respectively.Immunocytochemistry/Immunofluorescence: Dnmt3b Antibody (52A1018) [NB100-56514] - Dnmt3b antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.Flow Cytometry: Dnmt3b Antibody (52A1018) [NB100-56514] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3b antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Mouse Monoclonal
Species Human, Mouse
Applications IP, IHC-P, IHC-Fr...MORE

59 Publications
NBP2-27098
Western Blot: Dnmt3L Antibody [NBP2-27098] - Analysis of Dnmt3L using Dnmt3L antibody. GST tagged full length recombinant human Dnmt3L probed with 1) prebleed and 2) Dnmt3L antibody at 2 ug/ml.  Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: Dnmt3L Antibody [NBP2-27098] - Analysis of Dnmt3L in human kidney using Dnmt3L antibody at 5 ug/ml.

Rabbit Polyclonal
Species Human, Primate
Applications WB, IHC-P

1 Publication
NB200-106
Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Mouse
Applications IHC-P, IHC-Fr, IHC...MORE

28 Publications
NB100-56326
Western Blot: MeCP2 Antibody [NB100-56326] - analysis of MeCP2 in HeLa cell lysate using this antibody.

Rabbit Polyclonal
Species Human
Applications WB

1 Publication
NB100-81656
Western Blot: MBD2 Antibody [NB100-81656] - Detection of Human MBD2 by Western Blot of  Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells. Antibodies: Affinity purified  rabbit anti-MBD2 antibody NB100-81656 used for IP at 3 mcg/mg  lysate. MBD2 was also immunoprecipitated by rabbit anti-  MBD2 antibody NB100-81657, which recognizes a downstream  epitope. Detection:  Chemiluminescence with an exposure time of 3 secondsImmunocytochemistry/Immunofluorescence: MBD2 Antibody [NB100-81656] - Human breast carcinoma Affinity purified rabbit anti-MBD2 used at a dilution of 1:100.  Detection: Red-fluorescent goat anti-rabbit IgG highly crossadsorbed Antibody used at a dilution of 1:100.Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-81656] - Human breast carcinoma  Affinity purified rabbit anti-MBD2 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly crossadsorbed Antibody used at a dilution of 1:100.

Rabbit Polyclonal
Species Human, Mouse
Applications ICC/IF, IHC, IHC-P...MORE

     1 Review

NB100-56340
Western Blot: HDAC1 Antibody [NB100-56340] - analysis of HDAC1 in 293 cell lysate with this antibody.

Rabbit Polyclonal
Species Human
Applications WB

3 Publications
NB500-171
Western Blot: Histone H3 Antibody [NB500-171] - Total histone H3 levels in MCF-7 and HCT-116 cells. Image from verified customer review.Immunohistochemistry: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.Western Blot: Histone H3 Antibody [NB500-171] - Western blot analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100.

Rabbit Polyclonal
Species Yeast, Xenopus, Rat...MORE
Applications IHC-P, IHC, ICC/IF...MORE

     2 Reviews

4 Publications
NBP2-29463
Immunohistochemistry-Paraffin: p21 Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human urinary transitional cell carcinoma stained with p21 antibody at 1:50 using peroxidase-conjugate and DAB chromogen.

Mouse Monoclonal
Species Primate, Rat, Mouse...MORE
Applications IP, IHC-P, IHC-Fr...MORE

8 Publications
NBP1-47804
Western Blot: Isocitrate dehydrogenase Antibody (2H9) [NBP1-47804] Analysis of extracts (35ug) from 9 different cell lines by using anti-Isocitrate dehydrogenasemonoclonal antibody.Immunocytochemistry/Immunofluorescence: Isocitrate dehydrogenase Antibody (2H9) [NBP1-47804] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Isocitrate dehydrogenase.Immunohistochemistry-Paraffin: Isocitrate dehydrogenase Antibody (2H9) [NBP1-47804] - Endometrium tissue.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC...MORE

     1 Review

H00171023-M05
Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Expression in MCF-7. Sandwich ELISA: ASXL1 Antibody (6E2) [H00171023-M05] - Detection limit for recombinant GST tagged ASXL1 is 0.3 ng/ml as a capture antibody.

Mouse Monoclonal
Species Human
Applications S-ELISA, ELISA, WB

1 Publication
NB100-2379
Western Blot: OCT4 Antibody [NB100-2379] - Detection of OCT4 in mouse brain lysate.Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NB100-2379] - OCT4 staining in NTERA-2 cells detected with a Dylight 488 labeled secondary antibody.Flow Cytometry: OCT4 Antibody [NB100-2379] - Staining of NTERA-2  cells using NB100-2379 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications ICC/IF, FLOW, WB

4 Publications
NBP2-32104
Western Blot: TET2 Antibody [NBP2-32104] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells.  Antibodies:  Affinity purified rabbit anti-TET2 antibody NBP2-32104  used for IP at 6 ug/mg lysate.  TET2 was also immunoprecipitated by rabbit anti-TET2 antibody BL16766.  For blotting immunoprecipitated TET2, NBP2-32104 was used at 1 ug/ml.  Detection: Chemiluminescence with an exposure time of 3 minutes.    Immunohistochemistry: TET2 Antibody [NBP2-32104] - FFPE section of human ovarian carcinoma.  Antibody: Affinity purified rabbit anti-TET2 NBP2-32104 used at a dilution of 1:5,000 (0.2 Detection: DAB Western Blot: TET2 Antibody [NBP2-32104] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells.  Antibodies:  Affinity purified rabbit anti-TET2 antibody NBP2-32104 used for WB at 0.1 ug/ml.  Detection: Chemiluminescence with an exposure time of 30 seconds.

Rabbit Polyclonal
Species Human
Applications WB, IP

     1 Review

NBP2-30087
Western Blot: Flt3/CD135 Antibody [NBP2-30087] - Western blot analysis of anti-FLT3 Pab (NBP2-30087) in HL-60 cell lysate. FLT3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry-Paraffin: Flt3/CD135 Antibody [NBP2-30087] - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Flow Cytometry: Flt3/CD135 Antibody [NBP2-30087] - FLT3 (CD135) Antibody (N-term) (NBP2-30087) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Rabbit Polyclonal
Species Human
Applications WB, FLOW, IHC-P

NB100-57386
Western Blot: p53 Antibody (H53C2) [NB100-57386] - p53 in carcinoma cell extract. Image from verified customer review.

Mouse Monoclonal
Species Human
Applications WB, IHC, IP

     1 Review

NBP1-86304
Immunohistochemistry-Paraffin: DPEP2 Antibody [NBP1-86304] Staining of human kidney shows strong cytoplasmic positivity in tubular and glomerular cells.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NB200-616
Western Blot: Dnmt2 Antibody [NB200-616] - Homogenized mouse brain cells. Image from verified customer review.Western Blot: Dnmt2 Antibody [NB200-616] - analysis of Dnmt2 in mouse ES cell lysate using Dnmt2 antibodyat 2 ug/ml.Western Blot: Dnmt2 Antibody [NB200-616] - Western blot analysis for Dnmt2a using NB200-616 at 2 ug/ml dilution against 10 ug of mouse testis lysates. A protein band of approximate mol. wt. of 42 kDa is detected.

Rabbit Polyclonal
Species Mouse, Human
Applications WB

     1 Review

2 Publications
NB110-61646
Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Human
Applications IP, IHC-P, IHC...MORE

Bioinformatics Tool for DNMT3A

Discover related pathways, diseases and genes to DNMT3A. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Blogs on DNMT3A

Check out the latest blog posts on DNMT3A.
Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.