DMRT1 Antibody 0.05 ml

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Western Blot: DMRT1 Antibody [H00001761-B01] - analysis of DMRT1 expression in transfected 293T cell line by DMRT1 MaxPab polyclonal antibody (B01). Lane 1: DMRT1 transfected lysate (39.50 KDa). Lane 2: Non-transfected lysate.
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Ordering Information: H00001761-B01

  • Catalog Number
    H00001761-B01
  • Displayed Format
    Unconjugated
  • Size(s)
    0.05 ml
  • Availability
  • Price
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DMRT1 Antibody Summary

Species Human
Tested Applications WB, ELISA
Clonality
Polyclonal
Host
Mouse
Gene
DMRT1
Purity
Whole antisera
Specificity
DMRT1 - doublesex and mab-3 related transcription factor 1,
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DMRT1 Antibody Details

Immunogen
DMRT1 (-, 1 a.a. ~ 373 a.a) full-length human protein.MPNDEAFSKPSTPSEAPHAPGVPPQGRAGGFGKASGALVGAASGSSAGGSSRGGGSGSGASDLGAGSKKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCNLIAERQRVMAAQVALRRQQAQEEELGISHPIPLPSAAELLVKRENNGSNPCLMTECSGTSQPPPASVPTTAASEGRMVIQDIPAVTSRGHVENTPDLVSDSTYYSSFYQPSLFPYYNNLYNCPQYSMALAADSASGEVGNPLGGSPVKN

Species Reactivity

Human.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

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Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
This is unpurified antiserum so it is not possible to supply the specific antibody concentration.
Unit Size
0.05 ml
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Preservative
No Preservative
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol DMRT1
Entrez
Uniprot

Alternate Names for DMRT1 Antibody

  • DM domain expressed in testis 1
  • DMT1DM domain expressed in testis protein 1
  • doublesex and mab-3 related transcription factor 1
  • doublesex- and mab-3-related transcription factor 1
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Related Products by Gene

Background

This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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FAQs for DMRT1 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)