Complement C3 Antibody 0.1 mg

Click image to view larger
Western Blot: Complement C3 Antibody [NBP1-32080] - A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human C3-transfected 293T cells. 5 % SDS-PAGE.
Immunocytochemistry/Immunofluorescence: Complement C3 Antibody [NBP1-32080] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Paraffin-embedded mouse brain, using antibody at 1:500 dilution.
Western Blot: Complement C3 Antibody [NBP1-32080] - (30 ug of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:1500
Western Blot: Complement C3 Antibody [NBP1-32080] - Lane A: Non-transfected 293T lysates, Lane B: C3 transfected 293T lysates with Complement C3 Antibody diluted at 1:5000.
Western Blot: Complement C3 Antibody [NBP1-32080] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: C3 transfected 293T lysates 5% SDS PAGE; antibody diluted at 1:5000.
Download Datasheet
See All Related

Ordering Information: NBP1-32080

  • Catalog Number
    NBP1-32080
  • Displayed Format
    Unconjugated
  • Size(s)
    0.1 mg
  • Availability
  • Price
    Shipping
  

Complement C3 Antibody Summary

Species Human, Mouse
Tested Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
C3
Purity
Immunogen affinity purified
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Complement C3 Antibody Details

Isotype
IgG
Immunogen
Recombinant fragment corresponding to a region within amino acids 1448 and 1655 of (Uniprot ID#P01024)
Localization
Secreted

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Molecular Weight
187 kDa

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Earn rewards points by submitting your review for Complement C3 Antibody (NBP1-32080). Be the first to review this product and earn double rewards points!

Publications

Earn rewards if you have published using Complement C3 Antibody (NBP1-32080).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
1 x PBS, 20% Glycerol (pH7)
Unit Size
0.1 mg
Concentration
0.96 mg/ml
Preservative
0.025% Proclin
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol C3
Entrez
Uniprot

Alternate Names for Complement C3 Antibody

  • AHUS5
  • ARMD9
  • ASP
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
  • complement C3
  • complement component 3
  • complement component C3
  • CPAMD1acylation-stimulating protein cleavage product
Show More

Related Products by Gene

Background

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptable to bacteria infection.

Reviews for Complement C3 Antibody (NBP1-32080) (0)

There are no reviews for Complement C3 Antibody (NBP1-32080).
By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image
Have you used Complement C3 Antibody (NBP1-32080)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Publications for Complement C3 Antibody (NBP1-32080) (0)

There are no publications for Complement C3 Antibody (NBP1-32080).
By submitting a publication earn 200 points for your submission points towards our Rewards Program.
Have you published using Complement C3 Antibody (NBP1-32080)? Earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for Complement C3 (4)

  1. I am trying to establish a method to measure mice C3 levels by nephelometry. I would be most grateful if you could provide me with some help, regarding the choice of the Ab. I totally understand that since such a method has never been tried, I do not expect any guaranties. 
    • We have never performed nephelometry in our lab, and do not have a protocol or advice to provide about this application. However, it seems to me that you should choose an antibody that is capable of recognizing its target in its folded conformation. Therefore, I would suggest trying an antibody that has been validated for ICC or IHC.
  2. At what molecular weight can we find the band when run on western blotting with and with out reducing agent?
    • The theoretical molecular weight of Complement C3 is 187kDa. With NBP1-32080, we typically see bands around 180kDa when testing in Western blot under reducing conditions. As we have not tested this antibody in non-reducing conditions we cannot say with certainty at what size you will see the band, but it will most likely be at a higher weight than the band in reducing conditions.
  3. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  4. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Customers Who Viewed This Item Also Viewed...

NBP2-34234
Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234] - Formalin-fixed, paraffin-embedded human kidney transplant tissue (20X) stained with Complement 4d MAb (C4D204).Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234] - Formalin-fixed, paraffin-embedded human kidney transplant tissue (10X) stained with Complement 4d MAb (C4D204).Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234] - Formalin-fixed, paraffin-embedded human kidney transplant tissue (40X) stained with Complement 4d Ab (C4D204).

Mouse Monoclonal
Species Human
Applications IHC-P, IHC-Fr, ICC/IF

NBP2-33604
Western Blot: hnRNP C1 + C2 Antibody [NBP2-33604] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG spImmunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody [NBP2-33604] - Immunofluorescent staining of human cell line HEK 293 shows positivity in nucleus but not nucleoli.Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP2-33604] - liver cancer

Rabbit Polyclonal
Species Human
Applications IHC-P, IHC, ICC/IF...MORE

NBP1-58984
Western Blot: Complement C2 Antibody [NBP1-58984] - Human Heart lysate, concentration 0.2-1 ug/ml.

Rabbit Polyclonal
Species Porcine, Guinea Pig, Equine...MORE
Applications WB

NBP1-79075
Immunohistochemistry-Paraffin: Complement C4 Antibody (A24-T) [NBP1-79075] - Complement C4 Antibody (A24-T) Formalin-fixed and paraffin-embedded human transplanted kidney stained with anti-C4d complement antibody shows diffuse strong positive immunostaining of peritubular and glomerular capillaries (A), indicating acute antibody mediated rejection and diffuse strong positive immunostaining of dilated peritubular moderate capillaries (B), indicating acute antibody mediated rejection.

Rabbit Monoclonal
Species Human
Applications IHC-P, IHC-Fr, IHC

NB120-11879

Mouse Monoclonal
Species Human
Applications IA, WB

NBP1-88527
Western Blot: ARHGAP4 Antibody [NBP1-88527] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells); Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).Immunocytochemistry/Immunofluorescence: ARHGAP4 Antibody [NBP1-88527] - Staining of human cell line U-2 OS shows no positivity.Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC...MORE

NBP1-88526
Western Blot: PFDN4 Antibody [NBP1-88526] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Negative control (vector only transfected HEK293T lysate)<br/>Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419198)Immunocytochemistry/Immunofluorescence: PFDN4 Antibody [NBP1-88526] - Staining of human cell line A-431 shows positivity in nucleus, nucleoli, vesicles & cell junctions.Immunohistochemistry-Paraffin: PFDN4 Antibody [NBP1-88526] - Staining of human tonsil shows strong cytoplasmic positivity in most cells.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC...MORE

NBP1-89985
Western Blot: Complement Factor B Antibody [NBP1-89985] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).Immunohistochemistry-Paraffin: Complement Factor B Antibody [NBP1-89985] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.Western Blot: Complement Factor B Antibody [NBP1-89985] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line EFO-21<br/>Lane 4: Human cell line A-431<br/>Lane 5: Human liver tissue<br/>Lane 6: Human tonsil tissue

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications IHC-P, IHC, WB

3 Publications
NBP2-33933
Western Blot: Factor H Antibody [NBP2-33933] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp<br/>Lane 4: Human plasma (IgG/HSA depleted)<br/>Lane 5: Human liver tissue<br/>Lane 6: Human tonsil tissueImmunohistochemistry-Paraffin: Factor H Antibody [NBP2-33933] - Immunohistochemical staining of human small intestine shows strong plasma positivity.

Rabbit Polyclonal
Species Human
Applications IHC-P, IHC, WB

NBP1-87492
Western Blot: C1q Antibody [NBP1-87492] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Negative control (vector only transfected HEK293T lysate)<br/>Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414262)Immunohistochemistry-Paraffin: C1q Antibody [NBP1-87492] - Staining of human lung shows strong cytoplasmic positivity in macrophages.

Rabbit Polyclonal
Species Human
Applications IHC-P, IHC, WB

1 Publication
NB600-41532

Goat Polyclonal
Species Mouse
Applications IHC-P, IHC, ICC/IF...MORE

21 Publications
NBP1-19532
Immunocytochemistry/Immunofluorescence: TNF alpha Antibody [NBP1-19532] - TNF alpha antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Immunohistochemistry-Paraffin: TNF alpha Antibody [NBP1-19532] - Immunohistochemical staining of TNF alpha in mouse intestine.

Rabbit Polyclonal
Species Rat, Mouse, Human
Applications IHC-P, IHC, ICC/IF...MORE

     1 Review

4 Publications
NBP2-22192
Western Blot: C-Reactive Protein Antibody (1G1) [NBP2-22192] - Western blot analysis using C-Reactive Protein mAb against human C-Reactive Protein recombinant protein. ( AA: 1-224, Expected MW is 51 kDa)Immunohistochemistry-Paraffin: C-Reactive Protein Antibody (1G1) [NBP2-22192] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using C-Reactive Protein mouse mAb with DAB staining.Flow Cytometry: C-Reactive Protein Antibody (1G1) [NBP2-22192] - C-Reactive Protein mouse mAb (green) and negative control (red).

Mouse Monoclonal
Species Human
Applications WB, ELISA, FLOW...MORE

H00005688-M01
Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.Immunocytochemistry/Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell.Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF...MORE

     1 Review

1 Publication
NBP2-30078
Western Blot: Complement C6 Antibody [NBP2-30078] - Western blot analysis of C6 Antibody (N-term) (NBP2-30078) in MDA-MB231 cell line lysates (35ug/lane). C6 (arrow) was detected using the purified Pab.Immunocytochemistry/Immunofluorescence: Complement C6 Antibody [NBP2-30078] - Confocal immunofluorescent analysis of C6 Antibody (N-term)(NBP2-30078) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Immunohistochemistry-Paraffin: Complement C6 Antibody [NBP2-30078] - Formalin-fixed and paraffin-embedded human breast carcinoma reacted with C6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Rabbit Polyclonal
Species Human
Applications IHC-P, ICC/IF, FLOW...MORE

H00001604-M01
Western Blot: CD55 Antibody (1G3) [H00001604-M01] - Expression in transfected 293T cell line. Lane 1: CD55 transfected lysate(41.4 KDa). Lane 2: Non-transfected lysate.Western Blot: CD55 Antibody (1G3) [H00001604-M01] - DAF monoclonal antibody (M01), clone 1G3 Western Blot analysis of DAF expression in HeLa ( Cat # L013V1 ).ELISA: CD55 Antibody (1G3) [H00001604-M01] - Detection limit for recombinant GST tagged DAF is approximately 0.03ng/ml as a capture antibody.

Mouse Monoclonal
Species Human
Applications WB, ELISA, PLA...MORE

1 Publication
NBP1-58975
Western Blot: Properdin Antibody [NBP1-58975] - Human Thymus lysate, concentration 0.2-1 ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB

NB600-1131
Western Blot: IL6 Antibody [NB600-1131] - Shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax ™ chemiluminescent substrate.Immunocytochemistry/Immunofluorescence: IL6 Antibody [NB600-1131] - IL-6 antibody was tested in Raw264.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry-Paraffin: IL6 Antibody [NB600-1131] - IHC staining of IL6 in human bladder cancer using DAB with hematoxylin counterstain.

Rabbit Polyclonal
Species Rat, Mouse, Human
Applications WB, ELISA, ICC/IF...MORE

6 Publications
NBP1-61567
Western Blot: C5AR1 Antibody [NBP1-61567] - Analysis of extracts from HeLa cells, treated with PMA 125ng/ml 30', using C5AR1  Antibody. The lane on the right is treated with the synthesized peptide.Immunocytochemistry/Immunofluorescence: C5AR1 Antibody [NBP1-61567] - Analysis of HeLa cells, using C5AR1  Antibody. The picture on the right is treated with the synthesized peptide.Immunohistochemistry-Paraffin: C5AR1 Antibody [NBP1-61567] - Analysis of paraffin-embedded human brain tissue, using C5AR1  Antibody. The picture on the right is treated with the synthesized peptide.

Rabbit Polyclonal
Species Human
Applications IHC-P, IHC, ICC/IF...MORE

1 Publication