Colorimetric GTPase Assay Kit (602-0121)
Colorimetric GTPase Assay Kit 480Tests
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Colorimetric GTPase Assay Kit 480Tests Summary: | | | | Specificity: | Colorimetric GTPase Assay Kit (480 tests)
The GTPase colorimetric assay kit employs a 96-well plate format with all reagents necessary for measuring GTPase activity. The kit contains specially purified Pi-free GTP to ensure the lowest possible background signals. It also contains PiColorLock Gold reagent (an improved malachite green formulation) with additives to prevent background signals arising out of nonenzymatic GTP hydrolysis. Assays can be read anywhere in the wavelength range 590-660nm.
Feature s that make the Colorimetric GTPase Assay Kit such a powerful tool:
- Stable signal
- High Linearity
- Low Background
- Very easy to use
- Colorimetric Assay
- Ultra-pure substrate
- Contains all reagents required for the assay
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| Publications: | Antibody has been mentioned in at least 2 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Colorimetric GTPase Assay Products | Unit Size: | 480 Tests |
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| Concentration: | Concentration is not relevant for this product. Please see the protocols for proper use of this product. |
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Packaging: | Storage: | Storage is content dependent. |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Colorimetric assays for GTPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate.
At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes. Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance.
Uniquely, Innova Biosciences GTPase assay kits contain highly purified GTP, which have had any free inorganic phosphate removed - this means that assays have the lowest possible background signal.
PiColorLock reagent may also be purchased separately for developing simple assays for any phosphate-generating enzyme. It is offered in both high (Gold) and standard sensitivity (ALS) formulations to suit almost any assay situation.
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Publications (2)Colorimetric GTPase Assay Kit 480Tests Product Specific:- García-Regalado A, Guzmán-Hernández ML, Ramírez-Rangel I, et al. Garcia-Regalado A, Guzman-Hernez ML, Ramirez-Rangel I, et al. G Protein-coupled Receptor-promoted Trafficking of G{beta}1{gamma}2 Leads to AKT Activation at Endosomes via a Mechanism Mediated by G{beta}1{gamma}2-Rab11a Interaction. Mol Biol Cell 2008;19(10):4188-4200. [PMID: 18701709]
- Villarroya M, Prado S, Esteve JM, et al. Characterization of Human GTPBP3, a GTP-Binding Protein Involved in Mitochondrial tRNA Modification. Molecular and Cellular Biology (2008), 28 (24) pp 7514-7531. [PMID: 18852288]
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